ID   PRIM_HHV7J              Reviewed;         861 AA.
AC   P52468;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   23-FEB-2022, entry version 72.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=U43;
OS   Human herpesvirus 7 (strain JI) (HHV-7) (Human T lymphotropic virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Roseolovirus.
OX   NCBI_TaxID=57278;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8709220; DOI=10.1128/jvi.70.9.5975-5989.1996;
RA   Nicholas J.;
RT   "Determination and analysis of the complete nucleotide sequence of human
RT   herpesvirus.";
RL   J. Virol. 70:5975-5989(1996).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; U43400; AAC54705.1; -; Genomic_DNA.
DR   PIR; T41945; T41945.
DR   RefSeq; YP_073783.1; NC_001716.2.
DR   GeneID; 3289501; -.
DR   KEGG; vg:3289501; -.
DR   Proteomes; UP000009246; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..861
FT                   /note="DNA primase"
FT                   /id="PRO_0000116110"
FT   ZN_FING         805..843
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            492
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            494
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   861 AA;  101074 MW;  706B74EB4A007EE3 CRC64;
     MTITVFATEY DPAHVIVNIL CKNPSEHLIF PIIVKYKPSK NVYFCMQTQK CKFSKRIETV
     FVCDAESLNF SYYVKNALPI KSEDIIHCLN TEETENLYKD FLLSHVPEGS ENIEFKSLVF
     FCKTIIIKHL TNKYLLPTSP FWFLSTYGQT EGMLLLTMYY YLFEEQKSTI ATTKNYVQCF
     TDKLGDMVFT YSSMSEFINI TLKSNYRKKF VSFSEYAKQK NIRDRKEFLY LDKQIDIFRN
     SVHLTNSFRV HYIYIAYSTA LEKNKFIKYS QLTSYDPTRS DTSQCQENMY ILGNSLHSDL
     ISIMKQYFNE DSYFQNYVEI KRMLNNKFQM QQYVYDINSN RNIMLVINSD QISKMVNKCN
     KHGEGYFTPI KLGLQGFLKI LASNKSILID GKPVTRRQYL HDQFSNPIPM FRVQMSYKNL
     YCFGSAESWY KNMGFDQVMQ FLPNEYISDE SLTSTFWLQD TTFLSDEIEK QFYVTRHEIF
     NEYLPVTNYI GDLDLPLQDS AIITESLFFS MCKLMRNVLI NAWQKIFPFI DKDAYPIFFF
     KTTCSNPENP LNHNVCYNEV ETAFCVCKKK IGLRIAIPIP QGTAIIGSEP LKQLSKIFNH
     LMCLNHDLMQ ILNSVIFPGE CFDTGIYNTG RCLRLGFMYK VDEENNRFLY GRLKPIFIVP
     EKMKKNFQDF VSMQLDLNNI LHHGTKDQTI TEIIYSIFDK ACPTEFSFID SRTKQLYHRK
     QSSLETLCLK YLHVNGFSET SCLSRDDLLM TFTRSIAWPQ MLKKNIQHCE ARTATQFQHV
     TFLKIDHKNI QLKKLQNGKL SDFSCLTRNH KGNRENVLVY LEFKVDNNRI LIILWSKCFT
     TKCKSNSKQV HSSVVLDSLN M