ID   PRIM_MUHVS              Reviewed;         964 AA.
AC   Q69153;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 65.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=UL70;
OS   Murid herpesvirus 1 (strain Smith) (MuHV-1) (Mouse cytomegalovirus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Muromegalovirus.
OX   NCBI_TaxID=10367;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7483291; DOI=10.1007/bf01724298;
RA   Messerle M., Rapp M., Lucin P., Koszinowski U.H.;
RT   "Characterization of a conserved gene block in the murine cytomegalovirus
RT   genome.";
RL   Virus Genes 10:73-80(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8971012; DOI=10.1128/jvi.70.12.8833-8849.1996;
RA   Rawlinson W.D., Farrell H.E., Barrell B.G.;
RT   "Analysis of the complete DNA sequence of murine cytomegalovirus.";
RL   J. Virol. 70:8833-8849(1996).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; L07319; AAA96665.1; -; Genomic_DNA.
DR   EMBL; U68299; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   Proteomes; UP000008774; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..964
FT                   /note="DNA primase"
FT                   /id="PRO_0000116116"
FT   REGION          807..844
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            527
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            529
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   964 AA;  109704 MW;  70605300E1D85864 CRC64;
     MTVVLFATEY DTPNIVVNML SETPTEHHLF PLMIKYKPSN RIEFVLQTQR CPDSTRVRPV
     FICDARRLSL SEYVSTNTPL PARVICAGID ADATRELYEH LFDRKKDETG HDEENGSAAR
     HLFSNLTSTL KCLVHYNRSA ILRYLNNTFL SPTFPSWFLS TYGTHEGTLI LTMSYYLFER
     TYSTIQTTRD YTKCFTADPG RNLFTYINMR DFMATMNGSR FRKQTVLFAV FAKARNARDR
     CELEYVDAKI NAFREESRLA ADSCVYYVYL AYRTALCREK FLQYCEHTAY DKNLPDDQQC
     AAEENYLGRS LDAELISIMN TYFSVEGYFG SYIHVDRAKL SPPHSYRGYD WNTEADTMVG
     YSSTATNLAI SLRKLNSTCE SLFSPLPPTL MGLLKLCASD RYVPRAEKSR KRTSGGREKE
     DETRVCRRNY LLNDTSRPIG PMPVFRVEMP EKRHVFCAVS AENWTRRLLP KDLMKNLPSE
     YVSDECLTDA VWLREDIAAL CEVGEQLYRT RHEMFNENLP VFNFVGDVDL KLREDLQGLS
     RQEVFDLCRA LRRTLIGAWR HLFPEVDPDS HPVFFFKSAC PQNAAGAADE AMLYGGGGYD
     EDDDPRPEHA AAMVDYGDAV RRPPFCVCRR KLGLRVIIPF PPRTAAIGAQ TLKRLAGILD
     HTLCLDRDLV CKLNAISHPG ECFDTGIYSH GRSIRMPLMY KLDEASGLIL HSRLNPIFIV
     PAGYRDRPAE FVLQQLCPQN LTHHGRPPQR DGSADQLTEV VLHITDRACA DSDGNFLQSR
     ARRAMSSGRL PLGPLLRAHL SLESGQSAPS LPTLVGRGGG GEGGASSDYE EERAVGSDEE
     EDDDDVENLQ AFARRIAWPA LLRHTRNHYR EEVQQQLEAA TVFTAVGRTC VAVKRGLYGR
     ARDFSCLARE TYTRQETVQV FLDIRGDQRR NVWATLWSRC FTRRCNSNAK QTHLSLKISL
     PSQY