PRIM_NPVAC
ID PRIM_NPVAC Reviewed; 266 AA.
AC P41417;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 29-SEP-2021, entry version 68.
DE RecName: Full=DNA primase;
DE EC=2.7.7.-;
DE AltName: Full=Late expression factor 1;
GN Name=LEF-1; ORFNames=ORF14;
OS Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX NCBI_TaxID=46015;
OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=L1;
RX PubMed=8497062; DOI=10.1128/jvi.67.6.3481-3488.1993;
RA Passarelli A.L., Miller L.K.;
RT "Identification and characterization of lef-1, a baculovirus gene involved
RT in late and very late gene expression.";
RL J. Virol. 67:3481-3488(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C6;
RX PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT virus.";
RL Virology 202:586-605(1994).
RN [3]
RP FUNCTION, AND INTERACTION WITH LEF-2.
RX PubMed=9060674; DOI=10.1128/jvi.71.4.3114-3119.1997;
RA Evans J.T., Leisy D.J., Rohrmann G.F.;
RT "Characterization of the interaction between the baculovirus replication
RT factors LEF-1 and LEF-2.";
RL J. Virol. 71:3114-3119(1997).
RN [4]
RP FUNCTION.
RX PubMed=11836407; DOI=10.1128/jvi.76.5.2287-2297.2002;
RA Mikhailov V.S., Rohrmann G.F.;
RT "Baculovirus replication factor LEF-1 is a DNA primase.";
RL J. Virol. 76:2287-2297(2002).
CC -!- FUNCTION: Plays an essential role in viral DNA replication. May
CC generates single-stranded DNA for both leading and lagging strand
CC synthesis. The primase initiates primer synthesis and thereby produces
CC large amount of short RNA primers on the lagging strand that the
CC polymerase elongates using dNTPs. {ECO:0000269|PubMed:11836407,
CC ECO:0000269|PubMed:9060674, ECO:0000305}.
CC -!- SUBUNIT: Interacts with LEF-2. {ECO:0000269|PubMed:9060674}.
CC -!- SIMILARITY: Belongs to the baculoviridae LEF-1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L09723; AAA46709.1; -; Genomic_DNA.
DR EMBL; L22858; AAA66644.1; -; Genomic_DNA.
DR PIR; F72851; F72851.
DR RefSeq; NP_054043.1; NC_001623.1.
DR GeneID; 1403846; -.
DR KEGG; vg:1403846; -.
DR Proteomes; UP000008292; Genome.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003896; F:DNA primase activity; IDA:UniProtKB.
DR GO; GO:0019079; P:viral genome replication; IDA:UniProtKB.
DR InterPro; IPR016658; DNA_primase_LEF1.
DR PIRSF; PIRSF016433; Viral_DNA_prim; 1.
PE 1: Evidence at protein level;
KW Early protein; Reference proteome; Transcription; Transcription regulation;
KW Transferase.
FT CHAIN 1..266
FT /note="DNA primase"
FT /id="PRO_0000132816"
FT REGION 244..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 266 AA; 30780 MW; 05ABF515A787D679 CRC64;
MLVCNYTQKR VDMMWDAIAY NDSRKYAFMT VNARWIHADR YFDTSAQLYS YIVQNKVSDV
HVKPLDDGGG REWVVDADYK NYVDEHDLML KIYIGATAFL LFYTEENVSR VMYTGNRGFH
LWLKFTDKFK ITSAQNVRVH RYKAFEKPAK LDSDYIQPGS FAHCVREAVR LYVPHMQDSN
LDALTLQYWP DVDRDIFCNV NKQIRAPYSY NYKGTKFSRC ITKELLDKLK QCYPGYGTGG
CGPVTTTTTP SPPKIGSMQT TTKSTT