ID   PRIM_PSHV1              Reviewed;        1293 AA.
AC   Q6UDM2;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   23-FEB-2022, entry version 51.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=UL52;
OS   Psittacid herpesvirus 1 (isolate Amazon parrot/-/97-0001/1997) (PsHV-1)
OS   (Pacheco's disease virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Iltovirus.
OX   NCBI_TaxID=670426;
OH   NCBI_TaxID=152276; Amazona oratrix (yellow-headed parrot).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16873243; DOI=10.1128/jvi.00134-06;
RA   Thureen D.R., Keeler C.L. Jr.;
RT   "Psittacid herpesvirus 1 and infectious laryngotracheitis virus:
RT   Comparative genome sequence analysis of two avian alphaherpesviruses.";
RL   J. Virol. 80:7863-7872(2006).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY372243; AAQ73688.1; -; Genomic_DNA.
DR   RefSeq; NP_944382.1; NC_005264.1.
DR   GeneID; 2656989; -.
DR   KEGG; vg:2656989; -.
DR   Proteomes; UP000006840; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..1293
FT                   /note="DNA primase"
FT                   /id="PRO_0000406841"
FT   ZN_FING         1234..1274
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            843
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            845
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   1293 AA;  139922 MW;  C468CAE0693AFE59 CRC64;
     MFGFLIGVFF DRREESKCEP LVKHAPAIEP EASEAENYPL SSSADDKILA EALDAVETTI
     LGSGSCLHVT DTAAMPPAGD SAAAAVMMGS HSPGDRLMIE ALESVEASRG LTHSADTCSQ
     RCSIALSAPQ SPQSRVPEGP IMDSGQIFAV SGAGEEENWI DDEDMALCAA DLQIDPIGDA
     QSVGGSTAAC LPPSQKEEIR ILYATDGYPV VCSLHLLLGQ ALLQELYVIA YSHVTEPSAH
     GGGLLRATFY LLAATGCERQ RQRVRPVFVC SFTGEAADKT REALREGKPL MVQDVIASLD
     EAQSLALHED LIIALGTICS AGKTAAAPGG DERNLSYNYV ASTLPGLRRT LASLMLQYET
     RVVAAFRRKY NASFSCPFWF VSKFGPSETS LVAVLRYYLL GATQDDGIAG SFDLQGVKDL
     AVTFPKPGAN ASGLTAARLN TFAEFSRFWC CSELAGPKAE KLFREYLKTR VSSDTLYVQS
     LDSLIEKYRH ALRLPAKDFV RFVYLAYHEG YNRAAVEAHM EEALRNGGRA ETAATRAQSE
     FFKGLREMCS MPEYFARNVD VAVKRLPVRV AEEHARLRSY GEIPRSGFWG FCWPSYDVSL
     RINKICAGPL CCLGTQLADA MVRAPPDDGD KGTCAETAAT IIHHDSDASG EEEATVKVCG
     AHMPRANKRA GDGTRISPAC NEADTRAPLA RLTALAYSDQ RPRGIMPVTL LLYSLDDTSS
     GADTAVKSEA KAQDRPVPVY RVAMPGGGQA FAVVARDQWD KTVTVDVFSG FMAPKSPPES
     NGPLGSDKDA AAVDDAWTRA LLARAWGTSY ARRCAANVGA SQLYLNRNEV LSDSLAVCNL
     PLDLDITLKP SSVGRPSMLT LHMAMRSVRE AIVLLWSLLF ADAEIDPDTY PVYFYKTQCD
     AAAGDEEASP QRRGVGGGDD DVAWGEEDVE IEEMWGGYYS QTAMNDDMIW DDAPDPQMDI
     DSDHPAHEPM DDGAGAAIEE MKTAAAARFC RCERKIGFRI CVPIPKPYAL AGLSVAKATS
     ALVQQAIVLQ EKFVEALDQF IADYEFVDSG VYSAGRSLRL PFFAKVNARG VMVGRLLPFL
     VFPPKCRDRS EFVAQHADPN NFHVGAARRD NPAPTLIITS VTVRRDVLAQ DNRAHGKPRA
     GTARLADALA AVGIVPGNQQ GTPGDACAVD ASWLLDSVAM PALREYIDEH FPRHAAEYRD
     ARTDCIRVYE GRICAALRRG GAGYMSGRRS NFTCLKYQHR GASMQTVIAS VVVAVNSQGL
     PYAALQTRCF ATKCGSNELQ TQFTVTLSAT KDV