ID   PRIM_SUHVK              Reviewed;         962 AA.
AC   Q85228;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 51.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=UL52;
OS   Suid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain
OS   Kaplan)).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=33703;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7637001; DOI=10.1128/jvi.69.9.5560-5567.1995;
RA   Baumeister J., Klupp B.G., Mettenleiter T.C.;
RT   "Pseudorabies virus and equine herpesvirus 1 share a nonessential gene
RT   which is absent in other herpesviruses and located adjacent to a highly
RT   conserved gene cluster.";
RL   J. Virol. 69:5560-5567(1995).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-5 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; X87246; CAA60690.1; -; Genomic_DNA.
DR   IntAct; Q85228; 1.
DR   PRIDE; Q85228; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Transferase; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..962
FT                   /note="DNA primase"
FT                   /id="PRO_0000116114"
FT   ZN_FING         894..932
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            534
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            536
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   962 AA;  103329 MW;  5175209AD4AAC840 CRC64;
     MPPSMIRVLY ATDGCAITYS LMLLTGQEPS GAVYVVSYAW DGAGLDEAFS LPGERAEAEL
     LARRPGVTFC LTGHVASVRV RPVFVAAATP AVVRALCRGE PLAARDVLEA MDEAATFALH
     DGLIAALTMV LEQVRPRTGN AEYAPERPLR SIAVGRRGLT SLFVHHETQT LAAFRRLYGN
     HNTPFWYVAR FGPEEKTLVL ATRLHLFHPR PAYDLRALKD LLLTYNPRVD PNPSGLDPAG
     LLSFAALSRF CCLSGYARGP AAAHAARYVD ERVRADRAEM GVLRDYISHD RGSLKLPDRE
     FVTYVYLAHF ESFNRARLRE HLDAVNVTDP AAPVGRSPLG ERAAAAFFRH VRAQLNIRDY
     VAQNVTPSVA RLAPAMGAGY VEDRTYAALA ADAPRGLCDA AAGLARRVTA VEERLAPHGW
     VRAPDEEQQQ PGADGAVLRR LLELAAAPGG GRARTALGAL LGLPDACPPA PVYRVELAHR
     RQAFAVLAGD AWGRATARRD AAPEMAAHEP AAQMYVSRHE VFNARLAVTN IVLDVDFRLA
     RPVPAGTLEA AMRGFRRAVL DALALLFPEA DGDWAAHPCY VYKSACPPGG ALAAAAGSAS
     AASSDDGLEE APWDDDAALA DFGAAPGDED WADWDAGVPA EVYTCDDDEV GVGIGGAGGD
     PGASALVRAP VPADERPPCG CRAKMGFRVC TPVPSPYAVA GADTVRGLAR VLQQAVLLER
     DFIEPMGPYL QDFTFVDTGV YAHGRSLRLP FFAKVDGGGC HGRLLPFGDA PPGFDDPRNF
     HFHARPAHAV TRVLHSLGGE YESFFERKAA RNREAFFARR TPLADMLRGL AVDAEDRRAL
     EAFVADVAMA PVLRHLDAHF NGRAHEYAGA TAQRVVAKPD WVLFQLCGSA RFSCLRARHA
     RSPPARTFVA LSVDAHDRLC ISLSQQCFAT KCGSNATRTI FTAEVGQSCS SAGARCTSSS
     SG