ID   PRIM_VZVO               Reviewed;        1083 AA.
AC   Q4JQW9; Q4JQZ2;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   23-FEB-2022, entry version 45.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   ORFNames=ORF6;
OS   Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=341980;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Human/Japan/P-Oka/1970, and
RC   Oka varicella vaccine Biken (V-Oka-Biken);
RX   PubMed=12388706; DOI=10.1128/jvi.76.22.11447-11459.2002;
RA   Gomi Y., Sunamachi H., Mori Y., Nagaike K., Takahashi M., Yamanishi K.;
RT   "Comparison of the complete DNA sequences of the Oka varicella vaccine and
RT   its parental virus.";
RL   J. Virol. 76:11447-11459(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oka varicella vaccine VarilRix (V-Oka-GSK), and
RC   Oka varicella vaccine Varivax (V-Oka-Merk);
RX   PubMed=18787000; DOI=10.1128/jvi.00777-08;
RA   Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M.,
RA   Vassilev V.;
RT   "Complete DNA sequences of two oka strain varicella-zoster virus genomes.";
RL   J. Virol. 82:11023-11044(2008).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; AB097932; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AB097933; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DQ008354; AAY57682.1; -; Genomic_DNA.
DR   EMBL; DQ008355; AAY57753.1; -; Genomic_DNA.
DR   IntAct; Q4JQW9; 2.
DR   ChEMBL; CHEMBL4523677; -.
DR   Proteomes; UP000002603; Genome.
DR   Proteomes; UP000008504; Genome.
DR   Proteomes; UP000008505; Genome.
DR   Proteomes; UP000008506; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Transferase; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..1083
FT                   /note="DNA primase"
FT                   /id="PRO_0000385150"
FT   ZN_FING         1022..1061
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            669
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            671
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   CONFLICT        945
FT                   /note="P -> S (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1083 AA;  122590 MW;  6079F0831054BDF0 CRC64;
     MDKSSKPTIR LLFATKGCAI SHSLLLLTGQ ISTEPLYVVS YTWTPDLDDV FVKNGREEIT
     QVIPTKRPRE VTENDEENQI MHLFCSRDVN VIFYLIGGFS TGDVRSRVWP IFFCCFKTQT
     DFKALYKALW YGAPLNPHII SDTLCISETF DIHSEVIQTL MVTTHHLNRK GLSDNGLCIT
     EATLCKLVKK SVGRQELTSL YAHYERQVLA AYRRLYWGYG CSPFWYIVRF GPSEKTLVLA
     TRYYLLQTDT SYNTLETPLY DLQAIKDLFL TYQVPALPNC SGYNISDLLS FDKLSMFCCS
     STYTRGLTAK NALSYILQRI HTDTTEIHAV SEYITNDRKG LKVPDREFVD YIYLAHFECF
     NRKQIADHLQ AVTYSDFVNK PVLLKSSNLG KRATANFFNH VRSRLNMRDY IKKNVICDVT
     ELGPEIGHKY TITKTYTLSL TYAAKPSKFI GVCDLATTLT RRVENIEKQF SPYGWSSTIP
     SNPPGFDELS NFEDSVVSAE ALRAANFAND TPNQSGRTGF DTSPGITKLL LFFSAATGIA
     THDVSILSYK TPLEALIGHS EVTGPMPVYR VALPQGAQAF AVIANDTWSS ITNRYTLPHE
     ARLIAEDLKQ INPCNFVAAS LRDMQLTLLL STSVKNVSKI SSNIPKDQLY INRNELFNTN
     LIITNLILDV DFHIRKPIPL GILHAGMRAF RHGILTAMQL LFPKAVVNPN KDPCYFYKTA
     CPEPTVEVLD DDNLLDITSH SDIDFYIENG ELYTCVEENY TEDVWFFDTQ TTSEVHTHAD
     VSNNENLHET LPCNCKEKIG FRVCVPIPNP YALVGSSTLK GFAQILQQAV LLEREFVEYI
     GPYLRDFSFI DTGVYSHGHS LRLPFFSKVT TTGTAVGQLL PFYVVPEQCI DILAFVTSHR
     NPANFHFHSR PQSNVPVQFI LHNLGGEYAE FFERKVARNK QIFSPPQISL TKALKERGVT
     CLDAFTLEAF VDSTILESIV EHIAVHFPGR DREYTLTSSK CIAIKRDWVL FQLICGTKGF
     TCLRYPHRGG RTAPRTFVSL RVDHHNRLCI SLAQQCFATK CDSNRMHTIF TLEVPNYPNL
     TSS