ID   PRIN2_ARATH             Reviewed;         179 AA.
AC   Q9XIK0;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Protein PLASTID REDOX INSENSITIVE 2, chloroplastic {ECO:0000303|PubMed:22211401};
DE   Flags: Precursor;
GN   Name=PRIN2 {ECO:0000303|PubMed:22211401};
GN   OrderedLocusNames=At1g10522 {ECO:0000312|Araport:AT1G10522};
GN   ORFNames=T10O24.14 {ECO:0000312|EMBL:AAD39574.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista-Mercan V.R., Kim C.J., Chen H., Quan R., De Los Reyes C.,
RA   Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=22211401; DOI=10.1111/j.1365-313x.2011.04865.x;
RA   Kindgren P., Kremnev D., Blanco N.E., de Dios Barajas Lopez J.,
RA   Fernandez A.P., Tellgren-Roth C., Kleine T., Small I., Strand A.;
RT   "The plastid redox insensitive 2 mutant of Arabidopsis is impaired in PEP
RT   activity and high light-dependent plastid redox signalling to the
RT   nucleus.";
RL   Plant J. 70:279-291(2012).
RN   [6]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH CSP41B.
RC   STRAIN=cv. Columbia;
RX   PubMed=25161659; DOI=10.3389/fpls.2014.00385;
RA   Kremnev D., Strand A.;
RT   "Plastid encoded RNA polymerase activity and expression of photosynthesis
RT   genes required for embryo and seed development in Arabidopsis.";
RL   Front. Plant Sci. 5:385-385(2014).
CC   -!- FUNCTION: Involved in redox-mediated retrograde signaling to
CC       synchronize the expression of photosynthetic genes from both the
CC       nuclear and plastidic genomes, especially in excess light conditions
CC       (PubMed:22211401). Required for full expression of genes transcribed by
CC       the plastid-encoded RNA polymerase (PEP) (PubMed:22211401,
CC       PubMed:25161659). Essential for embryo development (PubMed:25161659).
CC       {ECO:0000269|PubMed:22211401, ECO:0000269|PubMed:25161659}.
CC   -!- SUBUNIT: Binds DNA when in complex with CSP41b.
CC       {ECO:0000269|PubMed:25161659}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid
CC       {ECO:0000269|PubMed:22211401}.
CC   -!- DISRUPTION PHENOTYPE: Misregulation of photosynthesis-associated
CC       nuclear gene expression in response to excess light, and inhibition of
CC       photosynthetic electron transport (PubMed:22211401, PubMed:25161659).
CC       Defects in embryo development (PubMed:25161659). Dwarf pale plants
CC       (PubMed:22211401, PubMed:25161659). The csp41b-2 prin2-2 double mutant
CC       is embryo lethal (PubMed:25161659). {ECO:0000269|PubMed:22211401,
CC       ECO:0000269|PubMed:25161659}.
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DR   EMBL; AC007067; AAD39574.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE28589.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE28590.1; -; Genomic_DNA.
DR   EMBL; AY086634; AAM63692.1; -; mRNA.
DR   EMBL; BT030627; ABR46207.1; -; mRNA.
DR   RefSeq; NP_001077502.1; NM_001084033.2.
DR   RefSeq; NP_563872.1; NM_100927.4.
DR   AlphaFoldDB; Q9XIK0; -.
DR   STRING; 3702.AT1G10522.2; -.
DR   PaxDb; Q9XIK0; -.
DR   PRIDE; Q9XIK0; -.
DR   ProteomicsDB; 226166; -.
DR   EnsemblPlants; AT1G10522.1; AT1G10522.1; AT1G10522.
DR   EnsemblPlants; AT1G10522.2; AT1G10522.2; AT1G10522.
DR   GeneID; 837593; -.
DR   Gramene; AT1G10522.1; AT1G10522.1; AT1G10522.
DR   Gramene; AT1G10522.2; AT1G10522.2; AT1G10522.
DR   KEGG; ath:AT1G10522; -.
DR   Araport; AT1G10522; -.
DR   TAIR; locus:505006116; AT1G10522.
DR   eggNOG; ENOG502S2MJ; Eukaryota.
DR   HOGENOM; CLU_101160_1_0_1; -.
DR   InParanoid; Q9XIK0; -.
DR   OMA; VKWAQSH; -.
DR   OrthoDB; 1393473at2759; -.
DR   PhylomeDB; Q9XIK0; -.
DR   PRO; PR:Q9XIK0; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9XIK0; baseline and differential.
DR   GO; GO:0042644; C:chloroplast nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0042646; C:plastid nucleoid; IDA:TAIR.
DR   GO; GO:0000427; C:plastid-encoded plastid RNA polymerase complex; IMP:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR   GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR   GO; GO:0009642; P:response to light intensity; IMP:TAIR.
DR   InterPro; IPR039349; PRIN2.
DR   PANTHER; PTHR35987; PTHR35987; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; DNA-binding; Plastid; Reference proteome; Transcription;
KW   Transcription regulation; Transit peptide.
FT   TRANSIT         1..69
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           70..179
FT                   /note="Protein PLASTID REDOX INSENSITIVE 2, chloroplastic"
FT                   /id="PRO_0000437696"
SQ   SEQUENCE   179 AA;  20252 MW;  17020176364D328F CRC64;
     MASMHEALFS SRLLQVNSSF SFRCALPIIS SPAAVSCAIK STQFFKQRCR TKVRDFSLSS
     LSRRGFVCRA AEYKFPDPIP EFAEAETEKF RDHMLNKLSK RDLFEDSVDE IVGVCTEIFE
     TFLRSEYGGP GTLLVIPFID MADTLNEREL PGGPQAARAA IKWAQDHVDK DWKEWTGTD