ID   ATG16_ASHGO             Reviewed;         124 AA.
AC   Q755K3;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Autophagy protein 16;
GN   Name=ATG16; OrderedLocusNames=AFL186W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Stabilizes the ATG5-ATG12 conjugate which is necessary for
CC       autophagy. The ATG5-ATG12/ATG16 complex is required for efficient
CC       promotion of ATG8-conjugation to phosphatidylethanolamine and ATG8
CC       localization to the pre-autophagosomal structure (PAS). Recruits also
CC       ATG3 to the PAS. Involved in endoplasmic reticulum-specific autophagic
CC       process and is essential for the survival of cells subjected to severe
CC       ER stress (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer (By similarity). Part of the ATG5-ATG12/ATG16
CC       complex. Several units of each may be present in this complex (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250|UniProtKB:Q03818}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q03818}.
CC   -!- SIMILARITY: Belongs to the ATG16 family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS53188.1; -; Genomic_DNA.
DR   RefSeq; NP_985364.1; NM_210718.1.
DR   PDB; 6RGO; X-ray; 3.70 A; C/D=70-124.
DR   PDBsum; 6RGO; -.
DR   AlphaFoldDB; Q755K3; -.
DR   SMR; Q755K3; -.
DR   STRING; 33169.AAS53188; -.
DR   PRIDE; Q755K3; -.
DR   EnsemblFungi; AAS53188; AAS53188; AGOS_AFL186W.
DR   GeneID; 4621589; -.
DR   KEGG; ago:AGOS_AFL186W; -.
DR   eggNOG; ENOG502S6TV; Eukaryota.
DR   HOGENOM; CLU_158825_0_0_1; -.
DR   InParanoid; Q755K3; -.
DR   OMA; QLRNKDY; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0034274; C:Atg12-Atg5-Atg16 complex; IEA:EnsemblFungi.
DR   GO; GO:0061908; C:phagophore; IEA:EnsemblFungi.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0120095; C:vacuole-isolation membrane contact site; IEA:EnsemblFungi.
DR   GO; GO:0019776; F:Atg8 ligase activity; IEA:EnsemblFungi.
DR   GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR   GO; GO:0030674; F:protein-macromolecule adaptor activity; IEA:EnsemblFungi.
DR   GO; GO:1905037; P:autophagosome organization; IEA:EnsemblFungi.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IEA:EnsemblFungi.
DR   GO; GO:0006501; P:C-terminal protein lipidation; IEA:EnsemblFungi.
DR   GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IEA:EnsemblFungi.
DR   GO; GO:0044805; P:late nucleophagy; IEA:EnsemblFungi.
DR   GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IEA:EnsemblFungi.
DR   InterPro; IPR013923; Autophagy-rel_prot_16_dom.
DR   Pfam; PF08614; ATG16; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Autophagy; Coiled coil; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..124
FT                   /note="Autophagy protein 16"
FT                   /id="PRO_0000218587"
FT   COILED          42..107
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   124 AA;  14390 MW;  C5A88752FB1B2A5F CRC64;
     MDLHQLYLDR LRQRDRIEGN FCYLFEAAVV LETAQPPQDS RDLVAKSLRE ELHAHEQEIH
     KLKDIVHLRS KDAEKLNDEI ISLNIENSLL QDKLTALQAE YDKLIQRWLA KAQSEADAMN
     QGLA