ID   PRKAA_XENLA             Reviewed;         309 AA.
AC   Q7ZYA5;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Interferon-inducible double-stranded RNA-dependent protein kinase activator A homolog A;
GN   Name=prkra-a; Synonyms=prkra;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Activates eif2ak2/pkr in the absence of double-stranded RNA
CC       (dsRNA), leading to phosphorylation of eif2s1/efi2-alpha and inhibition
CC       of translation and induction of apoptosis. Required for siRNA
CC       production by dicer1 and for subsequent siRNA-mediated post-
CC       transcriptional gene silencing. Does not seem to be required for
CC       processing of pre-miRNA to miRNA by dicer1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with dicer1 and eif2ak2/pkr. Also able to
CC       interact with dsRNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PRKRA family. {ECO:0000305}.
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DR   EMBL; BC043873; AAH43873.1; -; mRNA.
DR   RefSeq; NP_001079500.1; NM_001086031.1.
DR   AlphaFoldDB; Q7ZYA5; -.
DR   SMR; Q7ZYA5; -.
DR   BioGRID; 97430; 1.
DR   MaxQB; Q7ZYA5; -.
DR   DNASU; 379187; -.
DR   GeneID; 379187; -.
DR   KEGG; xla:379187; -.
DR   CTD; 379187; -.
DR   Xenbase; XB-GENE-940708; prkra.L.
DR   OMA; KKIAKHR; -.
DR   OrthoDB; 1093169at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   Bgee; 379187; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR   GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR   GO; GO:0030422; P:siRNA processing; ISS:UniProtKB.
DR   CDD; cd19889; DSRM_PRKRA_rpt1; 1.
DR   CDD; cd19891; DSRM_PRKRA_rpt2; 1.
DR   CDD; cd19892; DSRM_PRKRA_rpt3; 1.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR033363; PRKRA.
DR   InterPro; IPR044465; PRKRA_DSRM_1.
DR   InterPro; IPR044466; PRKRA_DSRM_2.
DR   InterPro; IPR044467; PRKRA_DSRM_3.
DR   InterPro; IPR032478; Staufen_C.
DR   PANTHER; PTHR46205:SF2; PTHR46205:SF2; 1.
DR   Pfam; PF00035; dsrm; 2.
DR   Pfam; PF16482; Staufen_C; 1.
DR   SMART; SM00358; DSRM; 3.
DR   PROSITE; PS50137; DS_RBD; 3.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Reference proteome; Repeat; RNA-binding;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..309
FT                   /note="Interferon-inducible double-stranded RNA-dependent
FT                   protein kinase activator A homolog A"
FT                   /id="PRO_0000223612"
FT   DOMAIN          31..98
FT                   /note="DRBM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          123..191
FT                   /note="DRBM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          236..304
FT                   /note="DRBM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   309 AA;  34070 MW;  5367FC051E6802C9 CRC64;
     MSQERFPAAP KMSSEKPTSL DAMRATNPCE TPIQLLHEFG TKTGNHPVYT LEKAEGQAHN
     PSFTFRLVIG DITSLGEGPS KKTAKQKAAE FALNILRGDT SKCLPVTDTL RDPKKPPNQM
     QENPVGSLQE LAVQKGWRLP EYTVAQESGP PHKREFTITC RVETFVETGS GTSKQVAKRV
     AAEKLLTKFK TISTDNIPLN KLIGNKMGCT WDSMRNSSGE KISMLKRSPL SIPNTDYVKM
     LKDVAEELDF NLTYLDIDEL SVNGQYQCLA ELSTNPITVC HGTGISCGNA HNDAAHNALQ
     YLKIMCIKK