ID   PRKAB_XENLA             Reviewed;         298 AA.
AC   Q91836;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Interferon-inducible double-stranded RNA-dependent protein kinase activator A homolog B;
DE   AltName: Full=Double-stranded RNA-binding protein A;
DE   AltName: Full=XlRBPA;
GN   Name=prkra-b; Synonyms=rbpa;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], RNA-BINDING, ASSOCIATION WITH RIBOSOMES,
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=9230068; DOI=10.1083/jcb.138.2.239;
RA   Eckmann C.R., Jantsch M.F.;
RT   "Xlrbpa, a double-stranded RNA-binding protein associated with ribosomes
RT   and heterogeneous nuclear RNPs.";
RL   J. Cell Biol. 138:239-253(1997).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 112-180.
RX   PubMed=9857205; DOI=10.1093/emboj/17.24.7505;
RA   Ryter J.M., Schultz S.C.;
RT   "Molecular basis of double-stranded RNA-protein interactions: structure of
RT   a dsRNA-binding domain complexed with dsRNA.";
RL   EMBO J. 17:7505-7513(1998).
CC   -!- FUNCTION: Activates eif2ak2/pkr in the absence of double-stranded RNA
CC       (dsRNA), leading to phosphorylation of eif2s1/efi2-alpha and inhibition
CC       of translation and induction of apoptosis. Required for siRNA
CC       production by dicer1 and for subsequent siRNA-mediated post-
CC       transcriptional gene silencing. Does not seem to be required for
CC       processing of pre-miRNA to miRNA by dicer1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with dicer1 and eif2ak2/pkr. Also able to
CC       interact with dsRNA (By similarity). Associates with ribosomes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Nucleus {ECO:0000269|PubMed:9230068}. Nucleus, nucleolus
CC       {ECO:0000269|PubMed:9230068}.
CC   -!- TISSUE SPECIFICITY: Expressed in brain, heart, kidney, liver, nerve and
CC       spleen. {ECO:0000269|PubMed:9230068}.
CC   -!- SIMILARITY: Belongs to the PRKRA family. {ECO:0000305}.
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DR   EMBL; M96370; AAA49947.1; -; mRNA.
DR   PIR; S27945; S27945.
DR   PDB; 1DI2; X-ray; 1.90 A; A/B=112-180.
DR   PDBsum; 1DI2; -.
DR   AlphaFoldDB; Q91836; -.
DR   SMR; Q91836; -.
DR   EvolutionaryTrace; Q91836; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR   GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR   GO; GO:0030422; P:siRNA processing; ISS:UniProtKB.
DR   CDD; cd19889; DSRM_PRKRA_rpt1; 1.
DR   CDD; cd19891; DSRM_PRKRA_rpt2; 1.
DR   CDD; cd19892; DSRM_PRKRA_rpt3; 1.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR033363; PRKRA.
DR   InterPro; IPR044465; PRKRA_DSRM_1.
DR   InterPro; IPR044466; PRKRA_DSRM_2.
DR   InterPro; IPR044467; PRKRA_DSRM_3.
DR   InterPro; IPR032478; Staufen_C.
DR   PANTHER; PTHR46205:SF2; PTHR46205:SF2; 1.
DR   Pfam; PF00035; dsrm; 2.
DR   Pfam; PF16482; Staufen_C; 1.
DR   SMART; SM00358; DSRM; 3.
DR   PROSITE; PS50137; DS_RBD; 3.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Nucleus; Reference proteome; Repeat; RNA-binding;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..298
FT                   /note="Interferon-inducible double-stranded RNA-dependent
FT                   protein kinase activator A homolog B"
FT                   /id="PRO_0000065624"
FT   DOMAIN          20..87
FT                   /note="DRBM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          112..180
FT                   /note="DRBM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          225..293
FT                   /note="DRBM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   HELIX           113..124
FT                   /evidence="ECO:0007829|PDB:1DI2"
FT   STRAND          130..138
FT                   /evidence="ECO:0007829|PDB:1DI2"
FT   HELIX           140..142
FT                   /evidence="ECO:0007829|PDB:1DI2"
FT   STRAND          144..151
FT                   /evidence="ECO:0007829|PDB:1DI2"
FT   STRAND          154..162
FT                   /evidence="ECO:0007829|PDB:1DI2"
FT   HELIX           163..179
FT                   /evidence="ECO:0007829|PDB:1DI2"
SQ   SEQUENCE   298 AA;  32852 MW;  8624D02F3AA1AFB1 CRC64;
     MSSEKPTSLN AMRATNPCET PIQLLHEFGT KTGNHPVYTL EKAEGQAHNP SFTFRLVIGD
     ITSLGEGPSK KTPKQKAAEF ALNILRGDTS KCLPVTDTLR DPKKPPNQMQ ENPVGSLQEL
     AVQKGWRLPE YTVAQESGPP HKREFTITCR VETFVETGSG TSKQVAKRVA AEKLLTKFKT
     ISTDNIPLNK LIGNKMGCTW DSMRNSSGEK ISMLKRSPLS IPNTDYVKML KDVAEELDFN
     LTYLDIDELS VNGQYQCLAE LSTNPITVCH GTGISCGNAH NDAAHNALQY LKIMCIKK