ID   ATG16_GIBZE             Reviewed;         198 AA.
AC   I1RFS8;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2012, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Autophagy-related protein 16 {ECO:0000303|PubMed:28894236};
GN   Name=ATG16 {ECO:0000303|PubMed:28894236};
GN   ORFNames=FG02566, FGRAMPH1_01T06151;
OS   Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS   / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=229533;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=17823352; DOI=10.1126/science.1143708;
RA   Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA   Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA   Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA   Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA   Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA   Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA   Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA   Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT   "The Fusarium graminearum genome reveals a link between localized
RT   polymorphism and pathogen specialization.";
RL   Science 317:1400-1402(2007).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA   King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA   Hammond-Kosack K.E.;
RT   "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT   graminearum.";
RL   BMC Genomics 16:544-544(2015).
RN   [4]
RP   IDENTIFICATION, FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=28894236; DOI=10.1038/s41598-017-11640-z;
RA   Lv W., Wang C., Yang N., Que Y., Talbot N.J., Wang Z.;
RT   "Genome-wide functional analysis reveals that autophagy is necessary for
RT   growth, sporulation, deoxynivalenol production and virulence in Fusarium
RT   graminearum.";
RL   Sci. Rep. 7:11062-11062(2017).
CC   -!- FUNCTION: Stabilizes the ATG5-ATG12 conjugate (By similarity). The
CC       ATG5-ATG12/ATG16 complex is required for efficient promotion of ATG8-
CC       conjugation to phosphatidylethanolamine and ATG8 localization to the
CC       pre-autophagosomal structure (PAS) (By similarity). Recruits also ATG3
CC       to the PAS (By similarity). Involved in endoplasmic reticulum-specific
CC       autophagic process and is essential for the survival of cells subjected
CC       to severe ER stress (By similarity). Autophagy is required for proper
CC       vegetative growth, asexual/sexual reproduction, and full virulence
CC       (PubMed:28894236). Autophagy is particularly involved in the
CC       biosynthesis of deoxynivalenol (DON), an important virulence
CC       determinant (PubMed:28894236). {ECO:0000250|UniProtKB:Q03818,
CC       ECO:0000269|PubMed:28894236}.
CC   -!- SUBUNIT: Homodimer (By similarity). Part of the ATG5-ATG12/ATG16
CC       complex (By similarity). Several units of each may be present in this
CC       complex (By similarity). Interacts directly with ATG12 (By similarity).
CC       {ECO:0000250|UniProtKB:Q03818}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250|UniProtKB:Q03818}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q03818}.
CC   -!- DISRUPTION PHENOTYPE: Does not significantly decrease the growth rate
CC       under nutrient-rich conditions (PubMed:28894236). Strongly reduces
CC       conidiation (PubMed:28894236). Causes only mild infection in point-
CC       inoculated spikelets of flowering wheat heads and impairs the spreading
CC       to nearby spikelets (PubMed:28894236). Reduces also strongly the
CC       production of deoxynivalenol (DON), an important virulence determinant
CC       (PubMed:28894236). {ECO:0000269|PubMed:28894236}.
CC   -!- SIMILARITY: Belongs to the ATG16 family. {ECO:0000305}.
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DR   EMBL; HG970332; CEF74880.1; -; Genomic_DNA.
DR   RefSeq; XP_011318504.1; XM_011320202.1.
DR   AlphaFoldDB; I1RFS8; -.
DR   SMR; I1RFS8; -.
DR   STRING; 229533.I1RFS8; -.
DR   GeneID; 23549936; -.
DR   KEGG; fgr:FGSG_02566; -.
DR   VEuPathDB; FungiDB:FGRAMPH1_01G06151; -.
DR   eggNOG; ENOG502S5CU; Eukaryota.
DR   HOGENOM; CLU_082752_1_0_1; -.
DR   InParanoid; I1RFS8; -.
DR   Proteomes; UP000070720; Chromosome 1.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000045; P:autophagosome assembly; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR045160; ATG16.
DR   InterPro; IPR013923; Autophagy-rel_prot_16_dom.
DR   PANTHER; PTHR19878; PTHR19878; 1.
DR   Pfam; PF08614; ATG16; 1.
PE   3: Inferred from homology;
KW   Autophagy; Coiled coil; Membrane; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..198
FT                   /note="Autophagy-related protein 16"
FT                   /id="PRO_0000443914"
FT   COILED          24..177
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   198 AA;  22848 MW;  5A1330CF4E514F21 CRC64;
     MPNWRDQYLS GIKDAELNNP VNMELVQTCS QMADRISALE AEKNGLETLV TTNGKTTARP
     TEPSTNDPAV AQLKQDLAEA LRSKGVAEKR LRSSEEELLQ LRSKHKTNTR SIRDLTADKN
     SLTTRLKDRE YELREKRKFI EQVQDEMIAL NLQMSMAEKE RDKVKKENKE LVDRWMKRMA
     QEAEAMNLAN EPIFKKGR