ID   PRKRA_DANRE             Reviewed;         282 AA.
AC   B0V3F8; A4IGC9;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Interferon-inducible double-stranded RNA-dependent protein kinase activator A homolog;
GN   Name=prkra; ORFNames=si:ch73-57b20.1, zgc:162619;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Activates eif2ak2/pkr in the absence of double-stranded RNA
CC       (dsRNA), leading to phosphorylation of eif2s1/efi2-alpha and inhibition
CC       of translation and induction of apoptosis. Required for siRNA
CC       production by dicer1 and for subsequent siRNA-mediated post-
CC       transcriptional gene silencing. Does not seem to be required for
CC       processing of pre-miRNA to miRNA by dicer1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Interacts with dicer1 and eif2ak2/pkr. Also able to
CC       interact with dsRNA (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PRKRA family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAQ15450.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CU104719; CAQ15450.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BC135034; AAI35035.1; -; mRNA.
DR   RefSeq; NP_001082830.1; NM_001089361.1.
DR   AlphaFoldDB; B0V3F8; -.
DR   SMR; B0V3F8; -.
DR   STRING; 7955.ENSDARP00000115290; -.
DR   PaxDb; B0V3F8; -.
DR   PeptideAtlas; B0V3F8; -.
DR   PRIDE; B0V3F8; -.
DR   Ensembl; ENSDART00000036605; ENSDARP00000036697; ENSDARG00000076718.
DR   GeneID; 557370; -.
DR   KEGG; dre:557370; -.
DR   CTD; 8575; -.
DR   ZFIN; ZDB-GENE-050309-206; prkra.
DR   eggNOG; KOG3732; Eukaryota.
DR   GeneTree; ENSGT00940000157618; -.
DR   HOGENOM; CLU_048292_0_1_1; -.
DR   InParanoid; B0V3F8; -.
DR   PhylomeDB; B0V3F8; -.
DR   TreeFam; TF315953; -.
DR   Reactome; R-DRE-203927; MicroRNA (miRNA) biogenesis.
DR   Reactome; R-DRE-426486; Small interfering RNA (siRNA) biogenesis.
DR   PRO; PR:B0V3F8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 9.
DR   Bgee; ENSDARG00000076718; Expressed in ovary and 23 other tissues.
DR   ExpressionAtlas; B0V3F8; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016442; C:RISC complex; IBA:GO_Central.
DR   GO; GO:0070578; C:RISC-loading complex; IBA:GO_Central.
DR   GO; GO:0003725; F:double-stranded RNA binding; IBA:GO_Central.
DR   GO; GO:0008047; F:enzyme activator activity; IEA:InterPro.
DR   GO; GO:0035197; F:siRNA binding; IBA:GO_Central.
DR   GO; GO:0070920; P:regulation of production of small RNA involved in gene silencing by RNA; IBA:GO_Central.
DR   GO; GO:0030422; P:siRNA processing; ISS:UniProtKB.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR033363; PRKRA.
DR   InterPro; IPR032478; Staufen_C.
DR   PANTHER; PTHR46205:SF2; PTHR46205:SF2; 1.
DR   Pfam; PF00035; dsrm; 2.
DR   Pfam; PF16482; Staufen_C; 1.
DR   SMART; SM00358; DSRM; 3.
DR   PROSITE; PS50137; DS_RBD; 3.
DR   PROSITE; PS50835; IG_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding;
KW   RNA-mediated gene silencing.
FT   CHAIN           1..282
FT                   /note="Interferon-inducible double-stranded RNA-dependent
FT                   protein kinase activator A homolog"
FT                   /id="PRO_0000379456"
FT   DOMAIN          10..77
FT                   /note="DRBM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          99..167
FT                   /note="DRBM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   DOMAIN          209..277
FT                   /note="DRBM 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT   CONFLICT        93
FT                   /note="E -> G (in Ref. 2; AAI35035)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   282 AA;  30840 MW;  D6A77AC5A5897A65 CRC64;
     MAPQRSQDKT PIQLLHEYGI KISSAPKYEL IHADGDAHQP SFMFSVTIGE VTCKGRGSTK
     KAAKHEAAEA ALKLLKRDSQ IIDQRDNNGL SPEAGEASNP VGILQELAMQ RVWCLPEYVV
     FMETGPGHMK EFTIACRLEG LEETGSGSSK KLARRAAAEN MIAKLQSLSG SSEITWSPPS
     RVYVESLRNS TGEKVSLLKR TPLSLPNTDY IQMLLEISLE LGFQVTYIDI DELTVNGQYQ
     CLVELSTRPV TVCHGSGVTS SNAHNAAAHN ALQYIKMVAS KH