ID   PRLB_LYSEN              Reviewed;         178 AA.
AC   P00801;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Beta-lytic metalloendopeptidase;
DE            EC=3.4.24.32;
DE   AltName: Full=Beta-lytic protease;
OS   Lysobacter enzymogenes.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Lysobacter.
OX   NCBI_TaxID=69;
RN   [1]
RP   PROTEIN SEQUENCE, AND DISULFIDE BONDS.
RC   STRAIN=ATCC 29487 / DSM 2043 / BCRC 11654 / KCTC 12131 / LMG 8762 / VKM
RC   B-2235 / UASM 495 / Ly e1;
RA   Damaglou A.P., Allen L.C., Whitaker D.R.;
RL   Submitted (MAR-1974) to the PIR data bank.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Cleavage of N-acetylmuramoyl-|-Ala, and of the insulin B chain
CC         at 23-Gly-|-Phe-24 > 18-Val-|-Cys(SO3H).; EC=3.4.24.32;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 1 zinc ion per subunit.;
CC   -!- SIMILARITY: Belongs to the peptidase M23A family. {ECO:0000305}.
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DR   PIR; A00994; LYYXB4.
DR   AlphaFoldDB; P00801; -.
DR   SMR; P00801; -.
DR   STRING; 69.GLE_0833; -.
DR   MEROPS; M23.001; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.70.70.10; -; 1.
DR   InterPro; IPR011055; Dup_hybrid_motif.
DR   InterPro; IPR000841; Pept_M23A_Blytic.
DR   PRINTS; PR00933; BLYTICPTASE.
DR   SUPFAM; SSF51261; SSF51261; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hydrolase; Metal-binding;
KW   Metalloprotease; Protease; Zinc.
FT   CHAIN           1..178
FT                   /note="Beta-lytic metalloendopeptidase"
FT                   /id="PRO_0000165903"
FT   BINDING         120
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   BINDING         122
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   DISULFID        65..111
FT                   /evidence="ECO:0000269|Ref.1"
FT   DISULFID        155..168
FT                   /evidence="ECO:0000269|Ref.1"
SQ   SEQUENCE   178 AA;  19100 MW;  9EFCD3DD45FD2870 CRC64;
     SPNGLLQFPF PRGASWHVGG AHTNTGSGNY PMSSLDMSRG GGSNQNGNWV SASAAGGSFK
     RHSSCFAEIV HTGGWSTTYY HLMNIQYNTG ANVSMNTAIA NAPNTQAQAL CNGGQSTGPH
     QHWSLKQNGS FYHLNGTYLS GYRITATGSS YDTNCSRFYL TKNGQNYCYG YYVNPGPN