ID   PRLF_ECOL6              Reviewed;         111 AA.
AC   Q8FDB5;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Antitoxin PrlF;
GN   Name=prlF; Synonyms=sohA; OrderedLocusNames=c3884;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Labile antitoxin that binds to the YhaV toxin and neutralizes its
CC       ribonuclease activity. Also acts as a transcription factor. The
CC       YhaV/PrlF complex binds the prlF-yhaV operon, probably negatively
CC       regulating its expression (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; forms a complex with YhaV with stoichiometry
CC       PrlF(2)-YhaV(4), possibly as a YhaV(2)-PrlF(2)-YhaV(2) complex like the
CC       MazFE complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the prlF-yhaV operon has no effect on
CC       virulence in mouse infection; the disrupted strain is as virulent as
CC       wild-type. {ECO:0000269|PubMed:23055930}.
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DR   EMBL; AE014075; AAN82325.1; -; Genomic_DNA.
DR   RefSeq; WP_001296435.1; NC_004431.1.
DR   AlphaFoldDB; Q8FDB5; -.
DR   STRING; 199310.c3884; -.
DR   EnsemblBacteria; AAN82325; AAN82325; c3884.
DR   GeneID; 66506893; -.
DR   KEGG; ecc:c3884; -.
DR   eggNOG; COG2002; Bacteria.
DR   HOGENOM; CLU_143957_0_0_6; -.
DR   OMA; DKICYTI; -.
DR   BioCyc; ECOL199310:C3884-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0097351; F:toxin sequestering activity; IEA:InterPro.
DR   GO; GO:0001558; P:regulation of cell growth; IEA:InterPro.
DR   InterPro; IPR031848; PrlF_antitoxin.
DR   InterPro; IPR007159; SpoVT-AbrB_dom.
DR   InterPro; IPR037914; SpoVT-AbrB_sf.
DR   Pfam; PF15937; PrlF_antitoxin; 1.
DR   SUPFAM; SSF89447; SSF89447; 1.
DR   PROSITE; PS51740; SPOVT_ABRB; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Repressor; Toxin-antitoxin system; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..111
FT                   /note="Antitoxin PrlF"
FT                   /id="PRO_0000420798"
FT   DOMAIN          12..59
FT                   /note="SpoVT-AbrB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
SQ   SEQUENCE   111 AA;  12322 MW;  85201B7B093E2382 CRC64;
     MPANARSNAV LTTESKVTIR GQTTIPAPVR EALKLKPGLD SIHYEILPGG QVFMCRLGDE
     QEDHTMNAFL RFLDADIQNN PQKTRPFDIQ QGKKLVAGMD VNIDDEIGDD E