PRLHR_MOUSE
ID PRLHR_MOUSE Reviewed; 370 AA.
AC Q6VMN6; E9QJU3; Q059H6;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Prolactin-releasing peptide receptor;
DE Short=PrRP receptor;
DE Short=PrRPR;
DE AltName: Full=G-protein coupled receptor 10;
GN Name=Prlhr; Synonyms=Gm339, Gpr10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus;
RX PubMed=14742914; DOI=10.1385/jmn:22:1-2:93;
RA Gu W., Geddes B.J., Zhang C., Foley K.P., Stricker-Krongrad A.;
RT "The prolactin-releasing peptide receptor (GPR10) regulates body weight
RT homeostasis in mice.";
RL J. Mol. Neurosci. 22:93-103(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=16299503; DOI=10.1038/nn1585;
RA Laurent P., Becker J.A.J., Valverde O., Ledent C.,
RA de Kerchove d'Exaerde A., Schiffmann S.N., Maldonado R., Vassart G.,
RA Parmentier M.;
RT "The prolactin-releasing peptide antagonizes the opioid system through its
RT receptor GPR10.";
RL Nat. Neurosci. 8:1735-1741(2005).
CC -!- FUNCTION: Receptor for prolactin-releasing peptide (PrRP). Implicated
CC in lactation, regulation of food intake and pain-signal processing.
CC {ECO:0000269|PubMed:14742914}.
CC -!- SUBUNIT: Interacts through its C-terminal region with the PDZ domain-
CC containing proteins GRIP1, GRIP2 and PICK1. Interacts with PDZ domains
CC 4 and 5 of GRIP1 and with the PDZ domain of PICK1 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- DISRUPTION PHENOTYPE: Increased nociceptive thresholds, increased
CC stress-induced analgesia and reduced morphine tolerance.
CC {ECO:0000269|PubMed:16299503}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY338238; AAQ84215.1; -; mRNA.
DR EMBL; AC167713; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC170185; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC125649; AAI25650.1; -; mRNA.
DR EMBL; BC125651; AAI25652.1; -; mRNA.
DR CCDS; CCDS29940.1; -.
DR RefSeq; NP_963909.2; NM_201615.2.
DR RefSeq; XP_006527120.1; XM_006527057.2.
DR AlphaFoldDB; Q6VMN6; -.
DR SMR; Q6VMN6; -.
DR STRING; 10090.ENSMUSP00000063114; -.
DR GlyGen; Q6VMN6; 2 sites.
DR iPTMnet; Q6VMN6; -.
DR PhosphoSitePlus; Q6VMN6; -.
DR PaxDb; Q6VMN6; -.
DR PRIDE; Q6VMN6; -.
DR Antibodypedia; 18777; 304 antibodies from 29 providers.
DR Ensembl; ENSMUST00000051277; ENSMUSP00000063114; ENSMUSG00000045052.
DR GeneID; 226278; -.
DR KEGG; mmu:226278; -.
DR UCSC; uc008ibu.2; mouse.
DR CTD; 2834; -.
DR MGI; MGI:2135956; Prlhr.
DR VEuPathDB; HostDB:ENSMUSG00000045052; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000162008; -.
DR HOGENOM; CLU_009579_6_1_1; -.
DR InParanoid; Q6VMN6; -.
DR OMA; LAWPRKI; -.
DR OrthoDB; 1072979at2759; -.
DR PhylomeDB; Q6VMN6; -.
DR TreeFam; TF315303; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR BioGRID-ORCS; 226278; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q6VMN6; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q6VMN6; protein.
DR Bgee; ENSMUSG00000045052; Expressed in embryonic brain and 5 other tissues.
DR GO; GO:0005929; C:cilium; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IDA:MGI.
DR GO; GO:0042923; F:neuropeptide binding; IBA:GO_Central.
DR GO; GO:0008188; F:neuropeptide receptor activity; IBA:GO_Central.
DR GO; GO:0004983; F:neuropeptide Y receptor activity; IEA:InterPro.
DR GO; GO:0017046; F:peptide hormone binding; ISO:MGI.
DR GO; GO:0007631; P:feeding behavior; IMP:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0042445; P:hormone metabolic process; IMP:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001402; Prolrel_pep_rcpt.
DR PANTHER; PTHR24235:SF11; PTHR24235:SF11; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01018; PRPRECEPTOR.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..370
FT /note="Prolactin-releasing peptide receptor"
FT /id="PRO_0000069525"
FT TOPO_DOM 1..62
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..101
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..126
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..223
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..317
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..370
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 365..370
FT /note="Required for interaction with GRIP1, GRIP2 and
FT PICK1"
FT /evidence="ECO:0000250"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 134..211
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 209
FT /note="S -> R (in Ref. 1; AAQ84215 and 3; AAI25650/
FT AAI25652)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 41182 MW; 83341A7F96D09C39 CRC64;
MTSLSTETTG DPDLSSGLLP ASSTPANQSA EASEGNLSAT VPRAAAVTPF QSLQLVHQLK
GLIVMLYSIV VVVGLVGNCL LVLVIARVRR LHNVTNFLIG NLALSDVLMC AACVPLTLAY
AFEPRGWVFG GGLCHLVFFL QPVTVYVSVF TLTTIALDRY VVLVHPLRRR ISLRLSAYAV
LGIWALSAVL ALPAAVHTYH VELKPHDVSL CEEFWGSQER QRQIYAWGLL LGTYLLPLLA
ILLSYVRVSV KLRNRVVPGS VTQSQADWDR ARRRRTFCLL VVVVVVFAVC WLPLHIFNLL
RDLDPRAIDP YAFGLVQLLC HWLAMSSACY NPFIYAWLHD SFREELRKML LSWPRKIVPH
GQNMTVSVVI
