ATG16_SCHPO
ID ATG16_SCHPO Reviewed; 143 AA.
AC O94656; P78904; Q1L842;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Autophagy protein 16;
GN Name=atg16; ORFNames=SPBC405.05;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=PR745;
RX PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT "Identification of open reading frames in Schizosaccharomyces pombe
RT cDNAs.";
RL DNA Res. 4:363-369(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION.
RX PubMed=16169489; DOI=10.1016/j.cub.2005.07.059;
RA Gregan J., Rabitsch P.K., Sakem B., Csutak O., Latypov V., Lehmann E.,
RA Kohli J., Nasmyth K.;
RT "Novel genes required for meiotic chromosome segregation are identified by
RT a high-throughput knockout screen in fission yeast.";
RL Curr. Biol. 15:1663-1669(2005).
RN [4]
RP IDENTIFICATION, FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND
RP INTERACTION WITH ATG5.
RX PubMed=23950735; DOI=10.1371/journal.pgen.1003715;
RA Sun L.L., Li M., Suo F., Liu X.M., Shen E.Z., Yang B., Dong M.Q., He W.Z.,
RA Du L.L.;
RT "Global analysis of fission yeast mating genes reveals new autophagy
RT factors.";
RL PLoS Genet. 9:E1003715-E1003715(2013).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Stabilizes the atg5-atg12 conjugate and mediates the
CC formation of the 350 kDa complex, which is necessary for autophagy. The
CC atg5-atg12/atg16 complex is required for efficient promotion of atg8-
CC conjugation to phosphatidylethanolamine and atg8 localization to the
CC preautophagosomal structure (PAS). Involved in endoplasmic reticulum-
CC specific autophagic process and is essential for the survival of cells
CC subjected to severe ER stress. Required for meiotic chromosome
CC segregation. {ECO:0000269|PubMed:16169489,
CC ECO:0000269|PubMed:23950735}.
CC -!- SUBUNIT: Homodimer (By similarity). Interacts with atg5. {ECO:0000250,
CC ECO:0000269|PubMed:23950735}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC Preautophagosomal structure membrane; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q03818}.
CC -!- DISRUPTION PHENOTYPE: Impairs atg8-processing.
CC {ECO:0000269|PubMed:23950735}.
CC -!- SIMILARITY: Belongs to the ATG16 family. {ECO:0000305}.
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DR EMBL; D89255; BAA13916.1; -; mRNA.
DR EMBL; CU329671; CAB38604.1; -; Genomic_DNA.
DR PIR; T40426; T40426.
DR PIR; T43180; T43180.
DR RefSeq; NP_596308.1; NM_001022230.2.
DR AlphaFoldDB; O94656; -.
DR SMR; O94656; -.
DR BioGRID; 277058; 11.
DR STRING; 4896.SPBC405.05.1; -.
DR iPTMnet; O94656; -.
DR MaxQB; O94656; -.
DR PaxDb; O94656; -.
DR PRIDE; O94656; -.
DR EnsemblFungi; SPBC405.05.1; SPBC405.05.1:pep; SPBC405.05.
DR GeneID; 2540530; -.
DR KEGG; spo:SPBC405.05; -.
DR PomBase; SPBC405.05; atg16.
DR VEuPathDB; FungiDB:SPBC405.05; -.
DR HOGENOM; CLU_1807331_0_0_1; -.
DR OMA; WALQLEV; -.
DR PRO; PR:O94656; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000407; C:phagophore assembly site; IDA:PomBase.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006501; P:C-terminal protein lipidation; ISO:PomBase.
DR GO; GO:0016236; P:macroautophagy; IMP:PomBase.
DR GO; GO:0051321; P:meiotic cell cycle; IEP:PomBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Autophagy; Cell cycle; Coiled coil; Cytoplasm; Meiosis; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..143
FT /note="Autophagy protein 16"
FT /id="PRO_0000358934"
FT COILED 66..142
FT /evidence="ECO:0000255"
FT CONFLICT 9
FT /note="D -> E (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
FT CONFLICT 20
FT /note="V -> F (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="F -> S (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
FT CONFLICT 44
FT /note="Q -> P (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="S -> G (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="E -> Q (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="Q -> P (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
FT CONFLICT 117
FT /note="K -> Q (in Ref. 1; BAA13916)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 143 AA; 16892 MW; B4BAFBA5E3DA741E CRC64;
MELIKKIQDR DAAEKAYYDV IEPYSELLEF SFHKEFVSEE KVTQRTASSD SLNTIASENN
DENVINLEEF RQLKRNCDLY QRNLQKLQLL FKQQSQKNTL LEKQLSLQTE LNQEKDKRVK
ILQDELWALQ LEVAALERKS PNA
