PRLR_SHEEP
ID PRLR_SHEEP Reviewed; 581 AA.
AC O46561; O46569; O46573; O46574; P79203; P79205;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Prolactin receptor;
DE Short=PRL-R;
DE Short=oPR;
DE Flags: Precursor;
GN Name=PRLR;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND ALTERNATIVE SPLICING.
RC TISSUE=Liver, and Mammary gland;
RX PubMed=9343303; DOI=10.1677/jme.0.0190109;
RA Bignon C., Binart N., Ormandy C., Schuler L.A., Kelly P.A., Djiane J.;
RT "Long and short forms of the ovine prolactin receptor: cDNA cloning and
RT genomic analysis reveal that the two forms arise by different alternative
RT splicing mechanisms in ruminants and in rodents.";
RL J. Mol. Endocrinol. 19:109-120(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 61-395, ALTERNATIVE SPLICING, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Scottish blackface; TISSUE=Pituitary anterior lobe;
RX PubMed=9832462; DOI=10.1210/endo.139.12.6365;
RA Tortonese D.J., Brooks J., Ingleton P.M., McNeilly A.S.;
RT "Detection of prolactin receptor gene expression in the sheep pituitary
RT gland and visualization of the specific translation of the signal in
RT gonadotrophs.";
RL Endocrinology 139:5215-5223(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE OF 147-302, ALTERNATIVE SPLICING, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Corpus luteum, and Fetal liver;
RA Anthony R.V., Smith G.W., Duong A., Pratt S.L., Smith M.F.;
RT "Two forms of the prolactin receptor messenger ribonucleic acid are present
RT in ovine fetal liver and adult ovary.";
RL Endocrine 3:291-295(1995).
CC -!- FUNCTION: This is a receptor for the anterior pituitary hormone
CC prolactin.
CC -!- SUBUNIT: Interacts with SMARCA1. Interacts with NEK3 and VAV2 and this
CC interaction is prolactin-dependent. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=Long, L-OPR;
CC IsoId=O46561-1; Sequence=Displayed;
CC Name=2; Synonyms=Short, S-OPR;
CC IsoId=O46561-2; Sequence=VSP_001732, VSP_001733;
CC Name=3; Synonyms=Soluble;
CC IsoId=O46561-3; Sequence=VSP_001730, VSP_001731;
CC -!- TISSUE SPECIFICITY: Expressed in all tissues examined; liver,
CC pituitary, adrenal gland, ovary and fetal liver.
CC {ECO:0000269|PubMed:9832462, ECO:0000269|Ref.3}.
CC -!- DOMAIN: The WSXWS motif appears to be necessary for proper protein
CC folding and thereby efficient intracellular transport and cell-surface
CC receptor binding.
CC -!- DOMAIN: The box 1 motif is required for JAK interaction and/or
CC activation.
CC -!- SIMILARITY: Belongs to the type I cytokine receptor family. Type 1
CC subfamily. {ECO:0000305}.
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DR EMBL; AF041257; AAB96795.1; -; mRNA.
DR EMBL; AF041977; AAB96920.1; -; mRNA.
DR EMBL; AF041979; AAB97082.1; -; mRNA.
DR EMBL; AF042358; AAB97744.1; -; Genomic_DNA.
DR EMBL; AF042358; AAB97743.1; -; Genomic_DNA.
DR EMBL; AF041978; AAB96965.1; -; mRNA.
DR EMBL; Y10578; CAA71597.1; -; mRNA.
DR EMBL; Y10808; CAA71766.1; -; mRNA.
DR RefSeq; NP_001009204.1; NM_001009204.1. [O46561-3]
DR AlphaFoldDB; O46561; -.
DR SMR; O46561; -.
DR STRING; 9940.ENSOARP00000012276; -.
DR GeneID; 443020; -.
DR KEGG; oas:443020; -.
DR CTD; 19066; -.
DR eggNOG; ENOG502R22A; Eukaryota.
DR OrthoDB; 1852977at2759; -.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004925; F:prolactin receptor activity; IEA:InterPro.
DR CDD; cd00063; FN3; 2.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR015152; Growth/epo_recpt_lig-bind.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003528; Long_hematopoietin_rcpt_CS.
DR InterPro; IPR033230; PRLR.
DR PANTHER; PTHR23036:SF86; PTHR23036:SF86; 2.
DR Pfam; PF09067; EpoR_lig-bind; 1.
DR SMART; SM00060; FN3; 2.
DR SUPFAM; SSF49265; SSF49265; 2.
DR PROSITE; PS50853; FN3; 2.
DR PROSITE; PS01352; HEMATOPO_REC_L_F1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Membrane;
KW Metal-binding; Receptor; Reference proteome; Repeat; Signal; Transmembrane;
KW Transmembrane helix; Zinc.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..581
FT /note="Prolactin receptor"
FT /id="PRO_0000010982"
FT TOPO_DOM 25..237
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 259..581
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 27..127
FT /note="Fibronectin type-III 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 129..229
FT /note="Fibronectin type-III 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT REGION 323..388
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 462..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 215..219
FT /note="WSXWS motif"
FT MOTIF 267..275
FT /note="Box 1 motif"
FT COMPBIAS 323..349
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 211
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 212
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT CARBOHYD 59
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 132
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 36..46
FT /evidence="ECO:0000250"
FT DISULFID 75..86
FT /evidence="ECO:0000250"
FT VAR_SEQ 24..66
FT /note="GQSPPEKPKLIKCRSPGKETFTCWWEPGADGGLPTNYTLTYRK -> ASLYV
FT PGGKCSSVCTYMAYPFVGGIFLHMYLCVDQYLLLTVTS (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_001730"
FT VAR_SEQ 67..581
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_001731"
FT VAR_SEQ 286..296
FT /note="KGKSEELLRAL -> ISQPSRLVSVF (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_001732"
FT VAR_SEQ 297..581
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_001733"
FT CONFLICT 281
FT /note="I -> V (in Ref. 1; AAB97743/AAB97744)"
FT /evidence="ECO:0000305"
FT CONFLICT 387
FT /note="E -> K (in Ref. 2; CAA71597)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 581 AA; 65235 MW; EC534FDE538837A0 CRC64;
MKENAASRVL FILLLFLFAS LLNGQSPPEK PKLIKCRSPG KETFTCWWEP GADGGLPTNY
TLTYRKEGET LIHECPDYKT GGPNSCYFSK KYTSIWKMYV ITVSAINQMG ISSSDPLYVD
VTYIVEPEPP VNLTLELKHP EDRKPYLWIK WSPPTLTDVK SGWFSIQYEI RLKPEKATDW
ETHFAPKLTQ LKIFNLYPGQ KYLVQIRCKP DHGYWSEWSP ESFIQIPNDF PVKDTSMWIF
VGVLSAVICL IMVWAVALKG YSMVTCILPP VPGPKIKGFD IHLLEKGKSE ELLRALESQD
FLPTSDCEDL LMEFIEVDDS EDQHLMPHPS KEHMEQGVKP MHLDPDTDSG RGSCDSPSLL
SEKCDEPQAY PSKFHIPEGP EKLEDPETNH TCLQAPQSTS GEGKIPYFLA NGPKSSTWPF
PQPPSLYSPR YSYHNIADVC ELALGMAGTT ATLLDQTDQH AFKPSKTIET GGEGKAAKQS
ESEGYSSEPD QDMAWPLLQD KTPLFSAKPL EYVEIHKVSQ DGVLALFPKQ NEKVDAPETS
KEYSKVSRVT DSNILVLIPD LQAQNLTLLE ESAKKAPPAL P
