ID   PRL_HORSE               Reviewed;         229 AA.
AC   P12420; Q6UBP9;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2005, sequence version 2.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Prolactin;
DE            Short=PRL;
DE   Flags: Precursor;
GN   Name=PRL;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=12742553; DOI=10.1016/s0739-7240(03)00013-4;
RA   Clark R.J., Valderrama X.P., Furlan M.A., Chedrese P.J.;
RT   "Cloning and nucleotide sequence of the equine and elk pituitary pre-
RT   prolactin cDNA.";
RL   Domest. Anim. Endocrinol. 24:367-376(2003).
RN   [2]
RP   PROTEIN SEQUENCE OF 31-229.
RC   TISSUE=Pituitary;
RX   PubMed=3045032; DOI=10.1111/j.1399-3011.1988.tb00913.x;
RA   Lehrman S.R., Lahm H.W., Miedel M.C., Hulmes J.D., Li C.H.;
RT   "Primary structure of equine pituitary prolactin.";
RL   Int. J. Pept. Protein Res. 31:544-554(1988).
CC   -!- FUNCTION: Prolactin acts primarily on the mammary gland by promoting
CC       lactation.
CC   -!- SUBUNIT: Interacts with PRLR. {ECO:0000250|UniProtKB:P01236}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the somatotropin/prolactin family.
CC       {ECO:0000305}.
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DR   EMBL; AY373339; AAQ76548.1; -; mRNA.
DR   PIR; JK0016; LCHO.
DR   RefSeq; NP_001075365.1; NM_001081896.1.
DR   AlphaFoldDB; P12420; -.
DR   SMR; P12420; -.
DR   STRING; 9796.ENSECAP00000000120; -.
DR   PaxDb; P12420; -.
DR   GeneID; 100034034; -.
DR   KEGG; ecb:100034034; -.
DR   CTD; 5617; -.
DR   HOGENOM; CLU_088274_0_1_1; -.
DR   InParanoid; P12420; -.
DR   Proteomes; UP000002281; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005179; F:hormone activity; IBA:GO_Central.
DR   GO; GO:0005148; F:prolactin receptor binding; IBA:GO_Central.
DR   GO; GO:0007565; P:female pregnancy; IBA:GO_Central.
DR   GO; GO:0007595; P:lactation; IEA:UniProtKB-KW.
DR   GO; GO:0030879; P:mammary gland development; IBA:GO_Central.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IBA:GO_Central.
DR   GO; GO:1903489; P:positive regulation of lactation; IBA:GO_Central.
DR   GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; IBA:GO_Central.
DR   GO; GO:0031667; P:response to nutrient levels; IBA:GO_Central.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR001400; Somatotropin/Prolactin.
DR   InterPro; IPR018116; Somatotropin_CS.
DR   PANTHER; PTHR11417; PTHR11417; 1.
DR   Pfam; PF00103; Hormone_1; 1.
DR   PRINTS; PR00836; SOMATOTROPIN.
DR   SUPFAM; SSF47266; SSF47266; 1.
DR   PROSITE; PS00266; SOMATOTROPIN_1; 1.
DR   PROSITE; PS00338; SOMATOTROPIN_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Hormone;
KW   Lactation; Phosphoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000269|PubMed:3045032"
FT   CHAIN           31..229
FT                   /note="Prolactin"
FT                   /id="PRO_0000032915"
FT   MOD_RES         56
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P01239"
FT   MOD_RES         64
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P01239"
FT   MOD_RES         120
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P01239"
FT   CARBOHYD        61
FT                   /note="N-linked (GlcNAc...) asparagine; partial"
FT   DISULFID        34..41
FT                   /evidence="ECO:0000250"
FT   DISULFID        88..204
FT                   /evidence="ECO:0000250"
FT   DISULFID        221..229
FT                   /evidence="ECO:0000250"
FT   CONFLICT        119
FT                   /note="R -> K (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   229 AA;  26256 MW;  A7B891BA9ADE737C CRC64;
     MDKKRSSLKG SLLLLLLLVS DLLLCKSVAS LPICPSGAVN CQVSLRELFD RAVILSHYIH
     NLSSEMFNEF DKRYAQGRGF VTKAINSCHT SSLSTPEDKE QAQQIHHEDL LNLILRVLRS
     WNDPLYHLVS EVRGMQEAPE AILSKAIEIE EQNRRLLEGM EKIVGQVQPR IKENEVYSVW
     SGLPSLQMAD EDSRLFAFYN LLHCLRRDSH KIDNYLKLLK CRIVYDSNC