PRM2_BOVIN
ID PRM2_BOVIN Reviewed; 115 AA.
AC P19782; Q02097; Q28171; Q32L38;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 2.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Protamine-2;
DE AltName: Full=Sperm histone P2;
DE AltName: Full=Sperm protamine P2;
GN Name=PRM2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=2320417; DOI=10.1093/nar/18.5.1249;
RA Maier W.-M., Nussbaum G., Domenjoud L., Klemm U., Engel W.;
RT "The lack of protamine 2 (P2) in boar and bull spermatozoa is due to
RT mutations within the P2 gene.";
RL Nucleic Acids Res. 18:1249-1254(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RX PubMed=1390885; DOI=10.1016/0167-4781(92)90003-i;
RA Kremling H., Reinhart N., Schlosser M., Engel W.;
RT "The bovine protamine 2 gene: evidence for alternative splicing.";
RL Biochim. Biophys. Acta 1132:133-139(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Protamines substitute for histones in the chromatin of sperm
CC during the haploid phase of spermatogenesis. They compact sperm DNA
CC into a highly condensed, stable and inactive complex.
CC {ECO:0000250|UniProtKB:P07978}.
CC -!- SUBUNIT: Interacts with TDRP. {ECO:0000250|UniProtKB:P07978}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P07978}.
CC Chromosome {ECO:0000250|UniProtKB:P07978}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P19782-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P19782-2; Sequence=VSP_021840;
CC -!- TISSUE SPECIFICITY: Testis.
CC -!- PTM: Proteolytic processing into mature chains is required for histone
CC eviction during spermatogenesis. Transition proteins (TNP1 and TNP2)
CC are required for processing. {ECO:0000250|UniProtKB:P07978}.
CC -!- SIMILARITY: Belongs to the protamine P2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA42912.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X16559; CAA34558.1; -; mRNA.
DR EMBL; X60353; CAA42913.1; -; Genomic_DNA.
DR EMBL; X60353; CAA42912.1; ALT_INIT; Genomic_DNA.
DR EMBL; BC109783; AAI09784.1; -; mRNA.
DR PIR; S28937; S28937.
DR RefSeq; NP_776582.2; NM_174157.4. [P19782-2]
DR AlphaFoldDB; P19782; -.
DR STRING; 9913.ENSBTAP00000037295; -.
DR PaxDb; P19782; -.
DR GeneID; 281424; -.
DR KEGG; bta:281424; -.
DR CTD; 5620; -.
DR InParanoid; P19782; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR GO; GO:0006997; P:nucleus organization; IBA:GO_Central.
DR GO; GO:0007286; P:spermatid development; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR InterPro; IPR000492; PRM2.
DR PANTHER; PTHR21341; PTHR21341; 1.
DR Pfam; PF00841; Protamine_P2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromosome; Developmental protein; Differentiation;
KW DNA condensation; DNA-binding; Nucleosome core; Nucleus; Phosphoprotein;
KW Reference proteome; Spermatogenesis.
FT CHAIN 1..115
FT /note="Protamine-2"
FT /id="PRO_0000191594"
FT REGION 1..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..80
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..115
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 8
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P11248"
FT VAR_SEQ 79..99
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_021840"
FT CONFLICT 60..62
FT /note="RRP -> A (in Ref. 1; CAA34558)"
FT /evidence="ECO:0000305"
FT CONFLICT 75
FT /note="R -> S (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 78
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="G -> R (in Ref. 2; CAA42913/CAA42912)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 115 AA; 13646 MW; 4749080EF087308C CRC64;
MVRCHVKSPT ESPPGQQGSG QQGETEHPDQ ARELRPEDIP VYGRTHRGRY HYRHRSHTRR
RPYRRRRRRA CRHRRRRRGA AGPPCAPIPG TPQASRQGSG CRRMRRRRRR CGRQL
