ID   PRM2_PIG                Reviewed;          92 AA.
AC   P19757;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Protamine-2;
DE   AltName: Full=Sperm histone P2;
DE   AltName: Full=Sperm protamine P2;
GN   Name=PRM2;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   PubMed=2320417; DOI=10.1093/nar/18.5.1249;
RA   Maier W.-M., Nussbaum G., Domenjoud L., Klemm U., Engel W.;
RT   "The lack of protamine 2 (P2) in boar and bull spermatozoa is due to
RT   mutations within the P2 gene.";
RL   Nucleic Acids Res. 18:1249-1254(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1627265; DOI=10.1515/bchm3.1992.373.1.261;
RA   Keime S., Heitland K., Kumm S., Schloesser M., Hroch N., Holtz W.,
RA   Engel W.;
RT   "Characterization of four genes encoding basic proteins of the porcine
RT   spermatid nucleus and close linkage of three of them.";
RL   Biol. Chem. Hoppe-Seyler 373:261-270(1992).
CC   -!- FUNCTION: Protamines substitute for histones in the chromatin of sperm
CC       during the haploid phase of spermatogenesis. They compact sperm DNA
CC       into a highly condensed, stable and inactive complex.
CC       {ECO:0000250|UniProtKB:P07978}.
CC   -!- SUBUNIT: Interacts with TDRP. {ECO:0000250|UniProtKB:P07978}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P07978}.
CC       Chromosome {ECO:0000250|UniProtKB:P07978}.
CC   -!- TISSUE SPECIFICITY: Testis.
CC   -!- PTM: Proteolytic processing into mature chains is required for histone
CC       eviction during spermatogenesis. Transition proteins (TNP1 and TNP2)
CC       are required for processing. {ECO:0000250|UniProtKB:P07978}.
CC   -!- SIMILARITY: Belongs to the protamine P2 family. {ECO:0000305}.
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DR   EMBL; X16558; CAA34557.1; -; mRNA.
DR   EMBL; M80676; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S13132; S13132.
DR   RefSeq; NP_999417.1; NM_214252.1.
DR   AlphaFoldDB; P19757; -.
DR   STRING; 9823.ENSSSCP00000024012; -.
DR   PaxDb; P19757; -.
DR   PeptideAtlas; P19757; -.
DR   Ensembl; ENSSSCT00015071908; ENSSSCP00015028844; ENSSSCG00015053982.
DR   GeneID; 397486; -.
DR   KEGG; ssc:397486; -.
DR   CTD; 5620; -.
DR   eggNOG; ENOG502TD5P; Eukaryota.
DR   HOGENOM; CLU_175685_0_0_1; -.
DR   InParanoid; P19757; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   Genevisible; P19757; SS.
DR   GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0006997; P:nucleus organization; IBA:GO_Central.
DR   GO; GO:0007286; P:spermatid development; IBA:GO_Central.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   InterPro; IPR000492; PRM2.
DR   PANTHER; PTHR21341; PTHR21341; 1.
DR   Pfam; PF00841; Protamine_P2; 1.
PE   2: Evidence at transcript level;
KW   Chromosome; Developmental protein; Differentiation; DNA condensation;
KW   DNA-binding; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome;
KW   Spermatogenesis.
FT   CHAIN           1..92
FT                   /note="Protamine-2"
FT                   /id="PRO_0000191610"
FT   REGION          1..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        22..43
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        44..76
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         8
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P11248"
FT   MOD_RES         10
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P11248"
FT   CONFLICT        64
FT                   /note="R -> S (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        68
FT                   /note="Missing (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        74
FT                   /note="R -> W (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   92 AA;  11807 MW;  FD30DCC160136A9F CRC64;
     MVRCRVRSPS ESPQQGSGQQ RENERQDQDQ ELRPEDVPVY GRTHRGRYHY RHRSHTRRRR
     SCRRRRRRAC RHRRHRRGCR RIRRRRRCRR RL