PRM3_MOUSE
ID PRM3_MOUSE Reviewed; 101 AA.
AC Q62100; A3KMD8; Q5BKQ3;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protamine-3;
DE AltName: Full=Sperm protamine P3;
GN Name=Prm3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129;
RX PubMed=8720108;
RX DOI=10.1002/(sici)1098-2795(199601)43:1<1::aid-mrd1>3.0.co;2-w;
RA Schlueter G., Celik A.B., Obata R., Schlicker M., Hofferbert S.,
RA Schlung A., Adham I.M., Engel W.;
RT "Sequence analysis of the conserved protamine gene cluster shows that it
RT contains a fourth expressed gene.";
RL Mol. Reprod. Dev. 43:1-6(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Protamines substitute for histones in the chromatin of sperm
CC during the haploid phase of spermatogenesis. They compact sperm DNA
CC into a highly condensed, stable and inactive complex.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC -!- TISSUE SPECIFICITY: Testis.
CC -!- SIMILARITY: Belongs to the protamine P3 family. {ECO:0000305}.
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DR EMBL; Z47352; CAA87412.1; -; Genomic_DNA.
DR EMBL; AK132894; BAE21408.1; -; mRNA.
DR EMBL; BC061127; AAH61127.1; -; mRNA.
DR EMBL; BC090986; AAH90986.1; -; mRNA.
DR CCDS; CCDS27954.1; -.
DR RefSeq; NP_038666.2; NM_013638.2.
DR AlphaFoldDB; Q62100; -.
DR STRING; 10090.ENSMUSP00000059630; -.
DR PhosphoSitePlus; Q62100; -.
DR PaxDb; Q62100; -.
DR PRIDE; Q62100; -.
DR ProteomicsDB; 291742; -.
DR DNASU; 19120; -.
DR Ensembl; ENSMUST00000050864; ENSMUSP00000059630; ENSMUSG00000050058.
DR GeneID; 19120; -.
DR KEGG; mmu:19120; -.
DR UCSC; uc007yef.1; mouse.
DR CTD; 58531; -.
DR MGI; MGI:106601; Prm3.
DR VEuPathDB; HostDB:ENSMUSG00000050058; -.
DR eggNOG; ENOG502T3TU; Eukaryota.
DR GeneTree; ENSGT00390000001558; -.
DR HOGENOM; CLU_2398985_0_0_1; -.
DR InParanoid; Q62100; -.
DR OMA; RCAKLNT; -.
DR OrthoDB; 1628050at2759; -.
DR PhylomeDB; Q62100; -.
DR BioGRID-ORCS; 19120; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Prm3; mouse.
DR PRO; PR:Q62100; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q62100; protein.
DR Bgee; ENSMUSG00000050058; Expressed in seminiferous tubule of testis and 22 other tissues.
DR Genevisible; Q62100; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR026077; PRMP3.
DR PANTHER; PTHR14317; PTHR14317; 1.
PE 1: Evidence at protein level;
KW Chromosome; Developmental protein; Differentiation; DNA condensation;
KW DNA-binding; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..101
FT /note="Protamine-3"
FT /id="PRO_0000106638"
FT REGION 1..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..66
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..90
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 93
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64256"
FT CONFLICT 6
FT /note="A -> S (in Ref. 1; CAA87412)"
FT /evidence="ECO:0000305"
FT CONFLICT 13
FT /note="G -> EQ (in Ref. 1; CAA87412)"
FT /evidence="ECO:0000305"
FT CONFLICT 21
FT /note="S -> N (in Ref. 1; CAA87412)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 101 AA; 11166 MW; B218F683DCA81E61 CRC64;
MGSRCAKLST GHGPAQNTGH SRGHESSMKK LVACVSQDNF SLSSEGEEEE EDEEEEEEEE
EEEEEEQIPV KGKLLLLEPE KQESAEDGEA QPSPEPKQTH S
