ID   PRM3_RAT                Reviewed;         104 AA.
AC   Q64256;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Protamine-3;
DE   AltName: Full=Sperm protamine P3;
GN   Name=Prm3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8720108;
RX   DOI=10.1002/(sici)1098-2795(199601)43:1<1::aid-mrd1>3.0.co;2-w;
RA   Schlueter G., Celik A.B., Obata R., Schlicker M., Hofferbert S.,
RA   Schlung A., Adham I.M., Engel W.;
RT   "Sequence analysis of the conserved protamine gene cluster shows that it
RT   contains a fourth expressed gene.";
RL   Mol. Reprod. Dev. 43:1-6(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8521723; DOI=10.1159/000134138;
RA   Schlueter G., Engel W.;
RT   "The rat Prm3 gene is an intronless member of the protamine gene cluster
RT   and is expressed in haploid male germ cells.";
RL   Cytogenet. Cell Genet. 71:352-355(1995).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Protamines substitute for histones in the chromatin of sperm
CC       during the haploid phase of spermatogenesis. They compact sperm DNA
CC       into a highly condensed, stable and inactive complex.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Testis.
CC   -!- SIMILARITY: Belongs to the protamine P3 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z46939; CAA87063.1; -; Genomic_DNA.
DR   EMBL; S79939; AAB35760.1; -; mRNA.
DR   RefSeq; NP_001002855.1; NM_001002855.2.
DR   AlphaFoldDB; Q64256; -.
DR   STRING; 10116.ENSRNOP00000003451; -.
DR   iPTMnet; Q64256; -.
DR   PhosphoSitePlus; Q64256; -.
DR   PaxDb; Q64256; -.
DR   PRIDE; Q64256; -.
DR   Ensembl; ENSRNOT00000003451; ENSRNOP00000003451; ENSRNOG00000002564.
DR   GeneID; 442921; -.
DR   KEGG; rno:442921; -.
DR   UCSC; RGD:1587265; rat.
DR   CTD; 58531; -.
DR   RGD; 1587265; Prm3.
DR   eggNOG; ENOG502T3TU; Eukaryota.
DR   GeneTree; ENSGT00390000001558; -.
DR   HOGENOM; CLU_2398985_0_0_1; -.
DR   InParanoid; Q64256; -.
DR   OMA; RCAKLNT; -.
DR   OrthoDB; 1628050at2759; -.
DR   PhylomeDB; Q64256; -.
DR   PRO; PR:Q64256; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000002564; Expressed in testis and 2 other tissues.
DR   Genevisible; Q64256; RN.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0030317; P:flagellated sperm motility; ISO:RGD.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR   InterPro; IPR026077; PRMP3.
DR   PANTHER; PTHR14317; PTHR14317; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Developmental protein; Differentiation; DNA condensation;
KW   DNA-binding; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome;
KW   Spermatogenesis.
FT   CHAIN           1..104
FT                   /note="Protamine-3"
FT                   /id="PRO_0000106639"
FT   REGION          1..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        43..69
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        70..88
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         96
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
SQ   SEQUENCE   104 AA;  11450 MW;  0EFE1BFA0CEFC9CA CRC64;
     MGSRCAKLST GHGPAQNTGH SRGHESSMKK LVACVSQDNF SLSSEGEEEE EDEEDEEEED
     DDEEDEEEEQ IPVKGKLLLL EPEKQDGAED AVAQPSPEPK QKHS