PRM5_YEAST
ID PRM5_YEAST Reviewed; 318 AA.
AC P40476; D6VVH0;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Pheromone-regulated membrane protein 5;
GN Name=PRM5; OrderedLocusNames=YIL117C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP INDUCTION.
RX PubMed=10594829; DOI=10.1046/j.1365-2958.1999.01667.x;
RA Jung U.S., Levin D.E.;
RT "Genome-wide analysis of gene expression regulated by the yeast cell wall
RT integrity signalling pathway.";
RL Mol. Microbiol. 34:1049-1057(1999).
RN [5]
RP INDUCTION.
RX PubMed=11062271; DOI=10.1083/jcb.151.3.719;
RA Heiman M.G., Walter P.;
RT "Prm1p, a pheromone-regulated multispanning membrane protein, facilitates
RT plasma membrane fusion during yeast mating.";
RL J. Cell Biol. 151:719-730(2000).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129; SER-279; SER-282 AND
RP SER-288, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [9]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-314, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Expression is regulated by the cell integrity signaling
CC pathway and by pheromone. {ECO:0000269|PubMed:10594829,
CC ECO:0000269|PubMed:11062271}.
CC -!- SIMILARITY: Belongs to the PRM5 family. {ECO:0000305}.
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DR EMBL; Z38125; CAA86263.1; -; Genomic_DNA.
DR EMBL; AY558083; AAS56409.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08436.1; -; Genomic_DNA.
DR PIR; S48455; S48455.
DR RefSeq; NP_012149.1; NM_001179465.1.
DR AlphaFoldDB; P40476; -.
DR BioGRID; 34874; 37.
DR DIP; DIP-2690N; -.
DR IntAct; P40476; 2.
DR MINT; P40476; -.
DR STRING; 4932.YIL117C; -.
DR iPTMnet; P40476; -.
DR MaxQB; P40476; -.
DR PaxDb; P40476; -.
DR PRIDE; P40476; -.
DR EnsemblFungi; YIL117C_mRNA; YIL117C; YIL117C.
DR GeneID; 854689; -.
DR KEGG; sce:YIL117C; -.
DR SGD; S000001379; PRM5.
DR VEuPathDB; FungiDB:YIL117C; -.
DR eggNOG; ENOG502S625; Eukaryota.
DR GeneTree; ENSGT00940000176521; -.
DR HOGENOM; CLU_061224_0_0_1; -.
DR InParanoid; P40476; -.
DR OMA; VSHTNNE; -.
DR BioCyc; YEAST:G3O-31370-MON; -.
DR PRO; PR:P40476; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40476; protein.
DR GO; GO:0005935; C:cellular bud neck; IBA:GO_Central.
DR GO; GO:0000324; C:fungal-type vacuole; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISM:SGD.
PE 1: Evidence at protein level;
KW Isopeptide bond; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Ubl conjugation.
FT CHAIN 1..318
FT /note="Pheromone-regulated membrane protein 5"
FT /id="PRO_0000202963"
FT TRANSMEM 75..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 238..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..265
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..303
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 282
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 288
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT CROSSLNK 314
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 318 AA; 34651 MW; A07EFE7A31D32390 CRC64;
MTVITIAKRG LPKLTTSTSS TTTASSSSTI TSVASSSSSL PLLSNSTSSS IIPSITPPSR
NGNPYILDSG DMPNGTVFIV VGGIAGVIFL AILLWWVITT YSSHRLTRSV QDYESKMFST
QHTQFYGDSP YMDYPAKENF QDQVHISESD ISPGNKDESV KDALVSHTNN EKPFLSNFER
PLFSLASESN RNSLFISPTG DILYKTRLSK LYQESPRLLQ KPVIMTSDNV STNSLVSTIS
SSSASSLDNG NEKEVGEDIR KPAKIASSPS RKLLNSPESD GSVNRNHSKG NLLVVQSKRK
PTPSTYLEHM LEGKEQDE
