ATG18_COCIM
ID ATG18_COCIM Reviewed; 417 AA.
AC Q1DKJ3; J3K2F7;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Autophagy-related protein 18;
GN Name=ATG18; ORFNames=CIMG_09170;
OS Coccidioides immitis (strain RS) (Valley fever fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX NCBI_TaxID=246410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RS;
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT and their relatives.";
RL Genome Res. 19:1722-1731(2009).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=RS;
RX PubMed=20516208; DOI=10.1101/gr.103911.109;
RA Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA Taylor J.W., Rounsley S.D.;
RT "Population genomic sequencing of Coccidioides fungi reveals recent
RT hybridization and transposon control.";
RL Genome Res. 20:938-946(2010).
CC -!- FUNCTION: The PI(3,5)P2 regulatory complex regulates both the synthesis
CC and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
CC Necessary for proper vacuole morphology. Plays an important role in
CC osmotically-induced vacuole fragmentation. Required for cytoplasm to
CC vacuole transport (Cvt) vesicle formation, pexophagy and starvation-
CC induced autophagy. Involved in correct ATG9 trafficking to the pre-
CC autophagosomal structure. Might also be involved in premeiotic DNA
CC replication (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the PI(3,5)P2 regulatory complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Endosome membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}.
CC -!- DOMAIN: The N-terminus might form a beta-propeller domain involved in
CC specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2),
CC leading to the association of the protein to the membrane.
CC {ECO:0000250}.
CC -!- DOMAIN: The L/FRRG motif is essential for the cytoplasm to vacuole
CC transport (Cvt) pathway, for the recruitment of ATG8 and ATG16 to the
CC PAS in nutrient-rich medium, and for its recruitment to and
CC dissociation from the PAS under starvation conditions.
CC {ECO:0000250|UniProtKB:P43601}.
CC -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR EMBL; GG704915; EAS27966.3; -; Genomic_DNA.
DR RefSeq; XP_001239549.1; XM_001239548.2.
DR AlphaFoldDB; Q1DKJ3; -.
DR SMR; Q1DKJ3; -.
DR STRING; 246410.Q1DKJ3; -.
DR EnsemblFungi; EAS27966; EAS27966; CIMG_09170.
DR GeneID; 4558284; -.
DR KEGG; cim:CIMG_09170; -.
DR VEuPathDB; FungiDB:CIMG_09170; -.
DR InParanoid; Q1DKJ3; -.
DR OMA; PSRDFAW; -.
DR OrthoDB; 1216824at2759; -.
DR Proteomes; UP000001261; Unassembled WGS sequence.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 3: Inferred from homology;
KW Autophagy; Endosome; Membrane; Protein transport; Reference proteome;
KW Repeat; Transport; Vacuole; WD repeat.
FT CHAIN 1..417
FT /note="Autophagy-related protein 18"
FT /id="PRO_0000318001"
FT REPEAT 1..36
FT /note="WD 1"
FT REPEAT 76..114
FT /note="WD 2"
FT REPEAT 185..225
FT /note="WD 3"
FT REPEAT 230..269
FT /note="WD 4"
FT REPEAT 300..346
FT /note="WD 5"
FT REPEAT 355..395
FT /note="WD 6"
FT REGION 267..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 226..230
FT /note="L/FRRG motif"
FT /evidence="ECO:0000250|UniProtKB:P43601"
SQ SEQUENCE 417 AA; 45539 MW; 3BB0AE7210CDD695 CRC64;
MSMNFVTFNQ DYSYLAVGTS KGFRIFTTDP FGKSYETKEG NIAILEMLFS TSLVAVILSP
RRLQIMNTKR QSVICELTFP TTVLAIRLNR KRLVIVLEDQ IYIYDIQTMK LVYTIETSPN
PNAICALAPS SDNCYLAYPL PQKAPPPSFS PPSHGPPSNT HIPPTSGEVL IFDAYKLEAV
NVVEAHKSPL SFLALNSEGT LLATASDKGT IIRVFSVPAA HKLYQFRRGS MPSRIYSMSF
NITSTLLCVS SATETIHIFK LGQQQGLSKT SSPSRKLESS RGSGDESAVE SASSEMSSRK
HNGTFMGMIR RTSQNVGNSF AATVGGYLPK GVTEMWEPER DFAWIKLPKS NGGNGGSGPV
RSVVAMSSNT PQVMVVTSEG NFYVFNIDLS KGGEGTLVKQ YSVLDSSDRM GSTDLDY
