ID   ATG18_CRYNB             Reviewed;         423 AA.
AC   P0CS29; Q55SA5; Q5KGQ2;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Autophagy-related protein 18;
GN   Name=ATG18; OrderedLocusNames=CNBE2770;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: The PI(3,5)P2 regulatory complex regulates both the synthesis
CC       and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
CC       Necessary for proper vacuole morphology. Plays an important role in
CC       osmotically-induced vacuole fragmentation. Required for cytoplasm to
CC       vacuole transport (Cvt) vesicle formation, pexophagy and starvation-
CC       induced autophagy. Involved in correct ATG9 trafficking to the pre-
CC       autophagosomal structure. Might also be involved in premeiotic DNA
CC       replication (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the PI(3,5)P2 regulatory complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC       Endosome membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}.
CC   -!- DOMAIN: The N-terminus might form a beta-propeller domain involved in
CC       specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2),
CC       leading to the association of the protein to the membrane.
CC       {ECO:0000250}.
CC   -!- DOMAIN: The L/FRRG motif is essential for the cytoplasm to vacuole
CC       transport (Cvt) pathway, for the recruitment of ATG8 and ATG16 to the
CC       PAS in nutrient-rich medium, and for its recruitment to and
CC       dissociation from the PAS under starvation conditions.
CC       {ECO:0000250|UniProtKB:P43601}.
CC   -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR   EMBL; AAEY01000024; EAL20916.1; -; Genomic_DNA.
DR   RefSeq; XP_775563.1; XM_770470.1.
DR   AlphaFoldDB; P0CS29; -.
DR   SMR; P0CS29; -.
DR   EnsemblFungi; EAL20916; EAL20916; CNBE2770.
DR   GeneID; 4936287; -.
DR   KEGG; cnb:CNBE2770; -.
DR   VEuPathDB; FungiDB:CNBE2770; -.
DR   HOGENOM; CLU_025895_5_2_1; -.
DR   Proteomes; UP000001435; Chromosome 5.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Autophagy; Endosome; Membrane; Protein transport; Repeat; Transport;
KW   Vacuole; WD repeat.
FT   CHAIN           1..423
FT                   /note="Autophagy-related protein 18"
FT                   /id="PRO_0000410327"
FT   REPEAT          200..240
FT                   /note="WD 1"
FT   REPEAT          245..284
FT                   /note="WD 2"
FT   REGION          281..317
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           241..245
FT                   /note="L/FRRG motif"
FT                   /evidence="ECO:0000250|UniProtKB:P43601"
SQ   SEQUENCE   423 AA;  45262 MW;  9EE8B1E9DB25F104 CRC64;
     MSRLAKRHPD LLSCNFNQDY SCIAVGHKKG YTILNCDPFG KVHSNNDQGA TGIVEMLFCT
     SLVALVGAAE NQPSNSPRKL QIVNTKRQST ICELIFPTSV LAVKMNRKRL IVVLENEIYI
     YDISTMKLLH TIETGPNPNA VCALSSSSER SYLAYPSPVP SASSTPLSSS AIPAPPPAPT
     TGDVLLFDTI SLTALNVIQA HKTPIAALAL NSTGTMLATA SDKGTVVRVF SVPDAKKLWQ
     FRRGSSSARI FSINFNLMST LLAVSSDTST IHIYRLASSR KGGKDADDAS TEEARSPTPS
     ETPLASSPPL AGGKLDSHSA ASSLRRRSYH LGKSFVGGVG GYLPKSVSEM WEPQRDFAFI
     KLRGNHGRTV VAMSATVPQV MVISSEGLFQ AYNIDLENGG ECSLMKEFAL LGSEDFGNGS
     NGI