ATG18_PICAN
ID ATG18_PICAN Reviewed; 525 AA.
AC Q5QA94;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Autophagy-related protein 18;
GN Name=ATG18;
OS Pichia angusta (Yeast) (Hansenula polymorpha).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Ogataea.
OX NCBI_TaxID=870730;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC Y-5445;
RX PubMed=15556634; DOI=10.1016/j.febslet.2004.10.055;
RA Leao-Helder A.N., Krikken A.M., Gellissen G., van der Klei I.J.,
RA Veenhuis M., Kiel J.A.K.W.;
RT "Atg21p is essential for macropexophagy and microautophagy in the yeast
RT Hansenula polymorpha.";
RL FEBS Lett. 577:491-495(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC Y-5445;
RX PubMed=14613885; DOI=10.1016/s1567-1356(03)00125-9;
RA Ramezani-Rad M., Hollenberg C.P., Lauber J., Wedler H., Griess E.,
RA Wagner C., Albermann K., Hani J., Piontek M., Dahlems U., Gellissen G.;
RT "The Hansenula polymorpha (strain CBS4732) genome sequencing and
RT analysis.";
RL FEMS Yeast Res. 4:207-215(2003).
CC -!- FUNCTION: The PI(3,5)P2 regulatory complex regulates both the synthesis
CC and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
CC Necessary for proper vacuole morphology. Plays an important role in
CC osmotically-induced vacuole fragmentation. Required for cytoplasm to
CC vacuole transport (Cvt) vesicle formation, pexophagy and starvation-
CC induced autophagy. Involved in correct ATG9 trafficking to the pre-
CC autophagosomal structure. Might also be involved in premeiotic DNA
CC replication (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the PI(3,5)P2 regulatory complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Endosome membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}.
CC -!- DOMAIN: The N-terminus might form a beta-propeller domain involved in
CC specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2),
CC leading to the association of the protein to the membrane.
CC {ECO:0000250}.
CC -!- DOMAIN: The L/FRRG motif is essential for the cytoplasm to vacuole
CC transport (Cvt) pathway, for the recruitment of ATG8 and ATG16 to the
CC PAS in nutrient-rich medium, and for its recruitment to and
CC dissociation from the PAS under starvation conditions.
CC {ECO:0000250|UniProtKB:P43601}.
CC -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR EMBL; AY756175; AAV74416.1; -; Genomic_DNA.
DR PDB; 5LTD; X-ray; 2.50 A; A/B=1-525.
DR PDB; 5LTG; X-ray; 2.00 A; A/B=1-525.
DR PDBsum; 5LTD; -.
DR PDBsum; 5LTG; -.
DR AlphaFoldDB; Q5QA94; -.
DR SMR; Q5QA94; -.
DR PhylomeDB; Q5QA94; -.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Autophagy; Endosome; Membrane; Protein transport; Repeat;
KW Transport; Vacuole; WD repeat.
FT CHAIN 1..525
FT /note="Autophagy-related protein 18"
FT /id="PRO_0000050870"
FT REPEAT 220..260
FT /note="WD 1"
FT REPEAT 265..304
FT /note="WD 2"
FT REGION 307..402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 261..265
FT /note="L/FRRG motif"
FT /evidence="ECO:0000250|UniProtKB:P43601"
FT COMPBIAS 319..333
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..362
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 369..385
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 386..402
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 35..39
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 43..50
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 53..58
FT /evidence="ECO:0007829|PDB:5LTG"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 63..69
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 73..79
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 82..91
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 100..105
FT /evidence="ECO:0007829|PDB:5LTG"
FT TURN 106..109
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 110..116
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 123..126
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 128..135
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 138..143
FT /evidence="ECO:0007829|PDB:5LTG"
FT TURN 144..146
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 149..154
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 173..177
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 202..208
FT /evidence="ECO:0007829|PDB:5LTG"
FT TURN 209..212
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 213..223
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 225..230
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 236..241
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 245..251
FT /evidence="ECO:0007829|PDB:5LTG"
FT TURN 252..254
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 257..262
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 270..275
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 279..286
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 290..295
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 440..444
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 453..466
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 486..497
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 500..507
FT /evidence="ECO:0007829|PDB:5LTG"
FT TURN 509..511
FT /evidence="ECO:0007829|PDB:5LTG"
FT STRAND 516..522
FT /evidence="ECO:0007829|PDB:5LTG"
SQ SEQUENCE 525 AA; 57936 MW; 17B8515CFE309952 CRC64;
MASPNPLAFE AATAAHEVAA SYVTEHKPRK NDNINFANFN QDFSCVSVGY SNGYKIYNCE
PFGQCYSKSD GSIGIVEMLF SSSLLAIVGM GEQHSLSPRR LKIINTKRQT TICELTFPGA
ILAVKLNRER LVVLLEETIY IYDINNMRLL HTIETPSNPN GLIALSPSSE NNYLAYPSPQ
KLAPNPQTEV TLHSNPQTVR NGDVIIFDAK TLQPTSVIEA HRTSLAAIAL SKDGLLLATA
SDKGTIIRVF SVATGIKLYQ FRRGTYPTKI YSLAFSPDNR FVIASSATET VHIFRLGEEE
AANTIKSANK KARLTKAQVP NPLETSPDIY PHNQHTSSDE DEELNEDEED LDGDEDEDLE
DDAHVPVSLQ RGRSSSSTGS FHSSESMTDK LKEPLVDNSR KSVARMLRRT SQSLGRKAAE
KMGTYLPPKF SSILEPNRHF ASLKVPASKE TKTVVGVGSK IWDDLIPSVY LKDDANSITE
TSEDLVNKKL VHIMVITSEG FFYKFGLDPE RGGDCVLLHQ QSLFG
