ID   PRNC_PSEFL              Reviewed;         567 AA.
AC   P95482;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Monodechloroaminopyrrolnitrin halogenase PrnC;
DE            EC=1.14.14.-;
GN   Name=prnC;
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   NOMENCLATURE.
RC   STRAIN=Bl915;
RX   PubMed=9172332; DOI=10.1128/aem.63.6.2147-2154.1997;
RA   Hammer P.E., Hill D.S., Lam S.T., Van Pee K.H., Ligon J.M.;
RT   "Four genes from Pseudomonas fluorescens that encode the biosynthesis of
RT   pyrrolnitrin.";
RL   Appl. Environ. Microbiol. 63:2147-2154(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Bl915;
RX   PubMed=9537395; DOI=10.1128/jb.180.7.1939-1943.1998;
RA   Kirner S., Hammer P.E., Hill D.S., Altmann A., Fischer I., Weislo L.J.,
RA   Lanahan M., van Pee K.H., Ligon J.M.;
RT   "Functions encoded by pyrrolnitrin biosynthetic genes from Pseudomonas
RT   fluorescens.";
RL   J. Bacteriol. 180:1939-1943(1998).
CC   -!- FUNCTION: Involved in the biosynthesis of the antifungal antibiotic
CC       pyrrolnitrin. Catalyzes the chlorination of
CC       monodechloroaminopyrrolnitrin (MDA) at the 3 position to form
CC       aminopyrrolnitrin (APRN). {ECO:0000269|PubMed:9172332,
CC       ECO:0000269|PubMed:9537395}.
CC   -!- PATHWAY: Antibiotic biosynthesis.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene lose the ability to
CC       produce pyrrolnitrin and accumulate MDA. {ECO:0000269|PubMed:9172332,
CC       ECO:0000269|PubMed:9537395}.
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DR   EMBL; U74493; AAB97506.1; -; Genomic_DNA.
DR   AlphaFoldDB; P95482; -.
DR   SMR; P95482; -.
DR   PRIDE; P95482; -.
DR   KEGG; ag:AAB97506; -.
DR   BioCyc; MetaCyc:MON-16707; -.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR006905; Flavin_halogenase.
DR   Pfam; PF04820; Trp_halogenase; 2.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   4: Predicted;
KW   Antibiotic biosynthesis; Monooxygenase; Oxidoreductase.
FT   CHAIN           1..567
FT                   /note="Monodechloroaminopyrrolnitrin halogenase PrnC"
FT                   /id="PRO_0000422332"
SQ   SEQUENCE   567 AA;  65038 MW;  EADB82C6269DFAE1 CRC64;
     MTQKSPANEH DSNHFDVIIL GSGMSGTQMG AILAKQQFRV LIIEESSHPR FTIGESSIPE
     TSLMNRIIAD RYGIPELDHI TSFYSTQRYV ASSTGIKRNF GFVFHKPGQE HDPKEFTQCV
     IPELPWGPES HYYRQDVDAY LLQAAIKYGC KVHQKTTVTE YHADKDGVAV TTAQGERFTG
     RYMIDCGGPR APLATKFKLR EEPCRFKTHS RSLYTHMLGV KPFDDIFKVK GQRWRWHEGT
     LHHMFEGGWL WVIPFNNHPR STNNLVSVGL QLDPRVYPKT DISAQQEFDE FLARFPSIGA
     QFRDAVPVRD WVKTDRLQFS SNACVGDRYC LMLHANGFID PLFSRGLENT AVTIHALAAR
     LIKALRDDDF SPERFEYIER LQQKLLDHND DFVSCCYTAF SDFRLWDAFH RLWAVGTILG
     QFRLVQAHAR FRASRNEGDL DHLDNDPPYL GYLCADMEEY YQLFNDAKAE VEAVSAGRKP
     ADEAAARIHA LIDERDFAKP MFGFGYCITG DKPQLNNSKY SLLPAMRLMY WTQTRAPAEV
     KKYFDYNPMF ALLKAYITTR IGLALKK