PRO1_CAEEL
ID PRO1_CAEEL Reviewed; 529 AA.
AC Q22006;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Pre-rRNA-processing protein pro-1;
DE AltName: Full=Proximal proliferation in germline protein 1;
GN Name=pro-1 {ECO:0000312|EMBL:CAA90666.1}; ORFNames=R166.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=14973273; DOI=10.1242/dev.01002;
RA Killian D.J., Hubbard E.J.A.;
RT "C. elegans pro-1 activity is required for soma/germline interactions that
RT influence proliferation and differentiation in the germ line.";
RL Development 131:1267-1278(2004).
RN [2] {ECO:0000312|EMBL:CAA90666.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=16876152; DOI=10.1016/j.ydbio.2006.06.011;
RA Voutev R., Killian D.J., Ahn J.H., Hubbard E.J.A.;
RT "Alterations in ribosome biogenesis cause specific defects in C. elegans
RT hermaphrodite gonadogenesis.";
RL Dev. Biol. 298:45-58(2006).
CC -!- FUNCTION: Component of the PELP1 complex involved in the nucleolar
CC steps of 28S rRNA maturation and the subsequent nucleoplasmic transit
CC of the pre-60S ribosomal subunit (By similarity). Required for
CC processing ITS2 sequences from rRNA intermediates during 26S rRNA
CC maturation (PubMed:16876152). Required in the soma to promote normal
CC proliferation and prevent germline tumor formation (PubMed:14973273).
CC {ECO:0000250|UniProtKB:Q9BV38, ECO:0000269|PubMed:14973273,
CC ECO:0000269|PubMed:16876152}.
CC -!- SUBUNIT: Component of the PELP1 complex, composed of at least PELP1,
CC TEX10 and WDR18. The complex interacts with pre-60S ribosome particles.
CC {ECO:0000250|UniProtKB:Q9BV38}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q9BV38}. Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q4VBE8}.
CC -!- DISRUPTION PHENOTYPE: Worms exhibit defects in rRNA processing which
CC disrupt hermaphrodite gonadogenesis and germline proliferation and
CC which are associated with a reduction in sheath cell number.
CC {ECO:0000269|PubMed:14973273}.
CC -!- SIMILARITY: Belongs to the WD repeat IPI3/WDR18 family. {ECO:0000305}.
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DR EMBL; Z50795; CAA90666.1; -; Genomic_DNA.
DR PIR; T24231; T24231.
DR RefSeq; NP_496271.1; NM_063870.4.
DR AlphaFoldDB; Q22006; -.
DR BioGRID; 39938; 1.
DR IntAct; Q22006; 1.
DR STRING; 6239.R166.4; -.
DR EPD; Q22006; -.
DR PaxDb; Q22006; -.
DR PeptideAtlas; Q22006; -.
DR EnsemblMetazoa; R166.4.1; R166.4.1; WBGene00004185.
DR GeneID; 174622; -.
DR KEGG; cel:CELE_R166.4; -.
DR UCSC; R166.4; c. elegans.
DR CTD; 174622; -.
DR WormBase; R166.4; CE03583; WBGene00004185; pro-1.
DR eggNOG; KOG0646; Eukaryota.
DR GeneTree; ENSGT00390000000289; -.
DR HOGENOM; CLU_038304_0_0_1; -.
DR InParanoid; Q22006; -.
DR OMA; EISSHQC; -.
DR OrthoDB; 600866at2759; -.
DR PhylomeDB; Q22006; -.
DR Reactome; R-CEL-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q22006; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00004185; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISS:WormBase.
DR GO; GO:0097344; C:Rix1 complex; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR GO; GO:0007281; P:germ cell development; IMP:WormBase.
DR GO; GO:0008406; P:gonad development; IMP:WormBase.
DR GO; GO:0007506; P:gonadal mesoderm development; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IMP:WormBase.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR045227; WDR18/Ipi3/RID3.
DR PANTHER; PTHR18763; PTHR18763; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Developmental protein; Differentiation;
KW Gonadal differentiation; Nucleus; Reference proteome; Repeat;
KW Ribosome biogenesis; rRNA processing; WD repeat.
FT CHAIN 1..529
FT /note="Pre-rRNA-processing protein pro-1"
FT /id="PRO_0000315877"
FT REPEAT 136..175
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 287..326
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REGION 436..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 416..518
FT /evidence="ECO:0000255"
FT COMPBIAS 436..452
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 529 AA; 58544 MW; DB541556D2C6AEC6 CRC64;
MAYLGVDTSR IAGTQPLEML LVSSGSPDPH STIIIDPRTG VSSWSYKGSE LQGASTGLVE
PLGHDGEHVV ITTKERPLVH VLAVHPKDRF HQKCVLPGPV SAIVSDNSGH FVFMSIKRQL
YCWLLSTGEL LSVIDAHYQN ITKIVISDDD SMIFTASKDG AVHGYLVTDL VSADRDHTVA
PFRKWASHTL SISDLKITNG SNPRVLSAGA DHIACLHSIS MDSVILKASS DRPLTACAID
PAETRIFIGT EVGNIAQINL FQLAPEERDL LVQAGDEHNT KFRVLNGHSD EISRLAINTD
GTLLASGDAS GKYCIWEISS HQCLKVSTMR SVINTLRFIP FWKTISGGEH VAKFRPVWDL
KREPTKCDRF AIEVSTEFNS DQKHWNDVIE ETIDQLLLES GSKSSAQLQW DAEGPARNEA
AKAEKAAENT IKNIITLGDD EDDAPEVGNQ RRKSGKKNKK NRKNQKKNDF EAAVIEKKQV
EPIVIDDEEE VNSSLQKKFD ALKAENEKLK EINKQMYEFV AKEIVDSEK
