ATG1T_ARATH
ID ATG1T_ARATH Reviewed; 408 AA.
AC F4I1N8; Q0WRR4; Q9M9B8;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Serine/threonine-protein kinase ATG1t {ECO:0000305};
DE EC=2.7.11.-;
DE AltName: Full=Autophagy-related protein 1t {ECO:0000305};
GN OrderedLocusNames=At1g49180 {ECO:0000312|Araport:AT1G49180};
GN ORFNames=F27J15.5 {ECO:0000312|EMBL:AAF69696.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Serine/threonine protein kinase involved in autophagy. The
CC ATG1-ATG13 protein kinase complex regulates downstream events required
CC for autophagosome enclosure and/or vacuolar delivery.
CC {ECO:0000250|UniProtKB:Q94C95}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome
CC {ECO:0000250|UniProtKB:Q94C95}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=F4I1N8-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF69696.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF00185.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAF00185.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC016041; AAF69696.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32400.1; -; Genomic_DNA.
DR EMBL; AK228237; BAF00185.1; ALT_SEQ; mRNA.
DR RefSeq; NP_175344.2; NM_103808.2. [F4I1N8-1]
DR AlphaFoldDB; F4I1N8; -.
DR SMR; F4I1N8; -.
DR STRING; 3702.AT1G49180.1; -.
DR PaxDb; F4I1N8; -.
DR PRIDE; F4I1N8; -.
DR EnsemblPlants; AT1G49180.1; AT1G49180.1; AT1G49180. [F4I1N8-1]
DR GeneID; 841341; -.
DR Gramene; AT1G49180.1; AT1G49180.1; AT1G49180. [F4I1N8-1]
DR KEGG; ath:AT1G49180; -.
DR Araport; AT1G49180; -.
DR TAIR; locus:2028496; AT1G49180.
DR eggNOG; KOG0595; Eukaryota.
DR eggNOG; KOG0969; Eukaryota.
DR HOGENOM; CLU_675020_0_0_1; -.
DR InParanoid; F4I1N8; -.
DR OrthoDB; 1084750at2759; -.
DR PRO; PR:F4I1N8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I1N8; baseline and differential.
DR Genevisible; F4I1N8; AT.
DR GO; GO:0005776; C:autophagosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR045269; Atg1-like.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR24348; PTHR24348; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Autophagy; Cytoplasmic vesicle; Kinase;
KW Nucleotide-binding; Protein transport; Reference proteome;
KW Serine/threonine-protein kinase; Transferase; Transport.
FT CHAIN 1..408
FT /note="Serine/threonine-protein kinase ATG1t"
FT /id="PRO_0000434622"
FT DOMAIN 7..272
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 129
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 13..21
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 36
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 408 AA; 46840 MW; 7311D328BFA21BA0 CRC64;
MMMLDDYIAK SKLSESLTST VWLAKHKLTG EEAVMKCFDL SKLNRNLRDC LNNELEFLSS
VDHPNIIRLL HVSQDDDFLV MVLEYCDGGT LSSYIQRYGR VEEDIAKRFM KQIGAGLEII
HDNHIIHRDL KPENILIDGS GDDLVLKIAD FSLARKLHPG KYLETVCGSP FYMAPEVLQF
QRYNEKADMW SVGAILFELL HGYPPFRGNN NVQVLRNIKS STALPFSRLI LQQMHPDCID
VCSRLLSINP AATLGIEDFP FLGRIKNSRV WVKDTTFSSR QHGTRERKVA TINGRLQFDL
SQDAYLPQRL LENLMYIYNT IEMARVTGVP TSYLISRGES IKVLSQLLRK AKQKTWFFQM
LRSWNLNKEL MKVQLYVGGF ISPTYSFTYS SITSNILINI SNDIYSHC
