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ATG1_KLULA
ID   ATG1_KLULA              Reviewed;         831 AA.
AC   Q6CSX2;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Serine/threonine-protein kinase ATG1 {ECO:0000250|UniProtKB:P53104};
DE            EC=2.7.11.1 {ECO:0000250|UniProtKB:P53104};
DE   AltName: Full=Autophagy-related protein 1 {ECO:0000250|UniProtKB:P53104};
GN   Name=ATG1 {ECO:0000250|UniProtKB:P53104};
GN   OrderedLocusNames=KLLA0C17160g {ECO:0000303|PubMed:15229592};
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Serine/threonine protein kinase involved in the cytoplasm to
CC       vacuole transport (Cvt) and found to be essential in autophagy, where
CC       it is required for the formation of autophagosomes. Involved in the
CC       clearance of protein aggregates which cannot be efficiently cleared by
CC       the proteasome. Required for selective autophagic degradation of the
CC       nucleus (nucleophagy) as well as for mitophagy which contributes to
CC       regulate mitochondrial quantity and quality by eliminating the
CC       mitochondria to a basal level to fulfill cellular energy requirements
CC       and preventing excess ROS production. Also involved in endoplasmic
CC       reticulum-specific autophagic process, in selective removal of ER-
CC       associated degradation (ERAD) substrates. Plays a key role in ATG9 and
CC       ATG23 cycling through the pre-autophagosomal structure and is necessary
CC       to promote ATG18 binding to ATG9 through phosphorylation of ATG9.
CC       Catalyzes phosphorylation of ATG4, decreasing the interaction between
CC       ATG4 and ATG8 and impairing deconjugation of PE-conjugated forms of
CC       ATG8. {ECO:0000250|UniProtKB:P53104}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC         Evidence={ECO:0000250|UniProtKB:P53104};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1; Evidence={ECO:0000250|UniProtKB:P53104};
CC   -!- SUBUNIT: Homodimer. Forms a ternary complex with ATG13 and ATG17.
CC       {ECO:0000250|UniProtKB:P53104}.
CC   -!- INTERACTION:
CC       Q6CSX2; Q6CWK2: ATG13; NbExp=4; IntAct=EBI-16151357, EBI-16151385;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P53104}.
CC       Preautophagosomal structure membrane {ECO:0000250|UniProtKB:P53104};
CC       Peripheral membrane protein {ECO:0000250|UniProtKB:P53104}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC       kinase family. APG1/unc-51/ULK1 subfamily. {ECO:0000255|PROSITE-
CC       ProRule:PRU00159}.
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DR   EMBL; CR382123; CAH01818.1; -; Genomic_DNA.
DR   RefSeq; XP_452967.1; XM_452967.1.
DR   AlphaFoldDB; Q6CSX2; -.
DR   SASBDB; Q6CSX2; -.
DR   SMR; Q6CSX2; -.
DR   DIP; DIP-61491N; -.
DR   IntAct; Q6CSX2; 1.
DR   STRING; 28985.XP_452967.1; -.
DR   PRIDE; Q6CSX2; -.
DR   EnsemblFungi; CAH01818; CAH01818; KLLA0_C17160g.
DR   GeneID; 2892164; -.
DR   KEGG; kla:KLLA0_C17160g; -.
DR   eggNOG; KOG0595; Eukaryota.
DR   HOGENOM; CLU_006447_1_0_1; -.
DR   InParanoid; Q6CSX2; -.
DR   OMA; INNVVQW; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:1990316; C:Atg1/ULK1 kinase complex; IEA:EnsemblFungi.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0097635; C:extrinsic component of autophagosome membrane; IEA:EnsemblFungi.
DR   GO; GO:0061908; C:phagophore; IEA:EnsemblFungi.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0120095; C:vacuole-isolation membrane contact site; IEA:EnsemblFungi.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000045; P:autophagosome assembly; IEA:EnsemblFungi.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IEA:EnsemblFungi.
DR   GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IEA:EnsemblFungi.
DR   GO; GO:0044805; P:late nucleophagy; IEA:EnsemblFungi.
DR   GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IEA:EnsemblFungi.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   GO; GO:0061709; P:reticulophagy; IEA:EnsemblFungi.
DR   InterPro; IPR045269; Atg1-like.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   InterPro; IPR022708; Ser/Thr_kinase_C.
DR   PANTHER; PTHR24348; PTHR24348; 1.
DR   Pfam; PF12063; DUF3543; 1.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Autophagy; Cytoplasm; Kinase; Membrane; Nucleotide-binding;
KW   Protein transport; Reference proteome; Serine/threonine-protein kinase;
KW   Transferase; Transport.
FT   CHAIN           1..831
FT                   /note="Serine/threonine-protein kinase ATG1"
FT                   /id="PRO_0000085647"
FT   DOMAIN          21..321
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          360..419
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        363..386
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        398..419
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        168
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10027"
FT   BINDING         27..35
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         50
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ   SEQUENCE   831 AA;  94026 MW;  E9C29F1492D9A489 CRC64;
     MSSESHDKVV AKAIRLPTEN YSVEKEIGKG SFAVVYKGLS LRDGRNIAIK AVSRSKLKNK
     KLLENLEVEI AILKKIKHPH IVGLIDCERT SSDFYLIMEY CALGDLTFFI KKRKNLVLKH
     PLIKTVFEHY PPPSTEHNGL NRVLVVNYLQ QLSSALKFLR SKNLVHRDIK PQNLLLCTPL
     LDYNDPKTFH ELGFVGIYNL PILKIADFGF ARFLPNTSLA ETLCGSPLYM APEILNYQKY
     NAKADLWSVG TVLYEMCCGR PPFKASNHLE LFQKIKKAND EITVPSNCYI EPKLFNLIRG
     LLTFDPDSRM GFTDFFNNEV VTEDLTRYEQ SYEPDLESKS KDVAESNMFV SEYLVKPLKQ
     QESAHIPPTQ TDENTSVQTG VRRTSGKERL ATNHPPHQQI HPEDNSQNPE QSYQSASQKR
     LKSSYNDLIL EKEYVVVEKK TVEVNSLADD FANNGPITNN QGAQVIKPLR YRTSSSSDAS
     GGRRASLVER RLSISSLSPS NALSKALGLA SVRLFGYQHN TKATSSPPQQ TLLNPQIFQE
     LTENAVLRAD HKLNPFSEQM LDSNITPAVE SLAAKAFVMY SFAEMKFSQI LPTPPSSTDY
     DPLSDKRLSN GSCAIEDEED LDQGRPPSNQ TLTSATTKIS SATNVDTQIP APELKKLCTE
     SLLLYLKALT ILAASMKLTS KWWYENESKN CTLKLNILVQ WIRDRFNECL DKAEFLRLKL
     HAINTSPNSQ WSDDDPVIFV EKLIYDRALD ISRNAARMEM ESGNYNTCEL AYATSLWMLE
     ILLDENFQFN EVYDDEYASN ITSLDESDKE MIKKYISSIA NRLKALKSKM V
 
 
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