ID   PROA_ORYSJ              Reviewed;         134 AA.
AC   Q0DN94; A0A0P0W417; P15839; Q40729; Q53X24;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=10 kDa prolamin;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os03g0766100, LOC_Os03g55730; ORFNames=OSJNBa0079B15;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare;
RA   Masumura T., Shibata D., Hibino T., Kato T., Kawabe K., Takeba G.,
RA   Tanaka K., Fujii S.;
RT   "cDNA cloning of an mRNA encording a sulfur-rich 10 kDa prolamin
RT   polypeptide in rice seeds.";
RL   Plant Mol. Biol. 12:123-130(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Newbonnet;
RX   PubMed=2308863; DOI=10.1093/nar/18.3.683;
RA   Feng G., Wen L., Huang J.K., Shorrosh B.S., Muthukrishnan S., Reeck G.R.;
RT   "Nucleotide sequence of a cloned rice genomic DNA fragment that encodes a
RT   10 kDa prolamin polypeptide.";
RL   Nucleic Acids Res. 18:683-683(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Zhonghua 8;
RA   You L., Xie M., Gu H., Qu L., Liang X., Chen Z.;
RT   "Cloning and sequence comparison of the gene encoding rice 10KD prolamin.";
RL   Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Tainung 67;
RA   Su P.-H., Chen C.-S.;
RT   "10 kDa Rice prolamin genomic clone.";
RL   Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ilpoombyeo;
RA   Yoon U.H., Kim Y.H.;
RT   "Molecular cloning of 10kDa prolamin genes in rice seeds.";
RL   Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [8]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [9]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- FUNCTION: Seed storage protein; serves as a source of nitrogen, carbon
CC       and sulfur for the young developing seedling.
CC   -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain. Note=In rice, prolamin
CC       accumulates as a type I protein body which originates directly from the
CC       endoplasmic reticulum.
CC   -!- SIMILARITY: Belongs to the prolamin family. {ECO:0000305}.
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DR   EMBL; X15231; CAA33301.1; -; mRNA.
DR   EMBL; X17074; CAA34926.1; -; Genomic_DNA.
DR   EMBL; L36605; AAA50319.1; -; Genomic_DNA.
DR   EMBL; AF294580; AAK84296.1; -; Genomic_DNA.
DR   EMBL; EF122438; ABL74525.1; -; mRNA.
DR   EMBL; AC099043; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP008209; BAF13294.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS86554.1; -; Genomic_DNA.
DR   PIR; S08219; S08219.
DR   AlphaFoldDB; Q0DN94; -.
DR   SMR; Q0DN94; -.
DR   IntAct; Q0DN94; 1.
DR   STRING; 4530.OS03T0766100-01; -.
DR   PRIDE; Q0DN94; -.
DR   EnsemblPlants; Os03t0766100-01; Os03t0766100-01; Os03g0766100.
DR   Gramene; Os03t0766100-01; Os03t0766100-01; Os03g0766100.
DR   HOGENOM; CLU_132896_0_0_1; -.
DR   InParanoid; Q0DN94; -.
DR   OMA; CMMQQPL; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q0DN94; OS.
DR   GO; GO:0033095; C:aleurone grain; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-KW.
DR   GO; GO:0019863; F:IgE binding; IEA:UniProt.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Seed storage protein; Signal; Storage protein; Vacuole.
FT   SIGNAL          1..24
FT   CHAIN           25..134
FT                   /note="10 kDa prolamin"
FT                   /id="PRO_0000032261"
FT   REGION          69..76
FT                   /note="Octapeptide unique to cereal prolamins"
SQ   SEQUENCE   134 AA;  14682 MW;  19320356E6B69F02 CRC64;
     MAAYTSKIFA LFALIALSAS ATTAITTMQY FPPTLAMGTM DPCRQYMMQT LGMGSSTAMF
     MSQPMALLQQ QCCMQLQGMM PQCHCGTSCQ MMQSMQQVIC AGLGQQQMMK MAMQMPYMCN
     MAPVNFQLSS CGCC