ATG20_YEAST
ID ATG20_YEAST Reviewed; 640 AA.
AC Q07528; D6VRN7;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Autophagy-related protein 20;
DE AltName: Full=Cytoplasm to vacuole targeting protein 20;
DE AltName: Full=Sorting nexin-42;
GN Name=ATG20; Synonyms=CVT20, SNX42; OrderedLocusNames=YDL113C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG17 AND SNX4, AND
RP MUTAGENESIS OF TYR-193.
RX PubMed=12048214; DOI=10.1074/jbc.m204736200;
RA Nice D.C. III, Sato T.K., Stromhaug P.E., Emr S.D., Klionsky D.J.;
RT "Cooperative binding of the cytoplasm to vacuole targeting pathway
RT proteins, Cvt13 and Cvt20, to phosphatidylinositol 3-phosphate at the pre-
RT autophagosomal structure is required for selective autophagy.";
RL J. Biol. Chem. 277:30198-30207(2002).
RN [4]
RP NOMENCLATURE.
RX PubMed=14536056; DOI=10.1016/s1534-5807(03)00296-x;
RA Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y.,
RA Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.;
RT "A unified nomenclature for yeast autophagy-related genes.";
RL Dev. Cell 5:539-545(2003).
RN [5]
RP FUNCTION, AND INTERACTION WITH SNX4.
RX PubMed=12554655; DOI=10.1093/emboj/cdg062;
RA Hettema E.H., Lewis M.J., Black M.W., Pelham H.R.B.;
RT "Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval
RT pathways from yeast endosomes.";
RL EMBO J. 22:548-557(2003).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH ATG11.
RX PubMed=15659643; DOI=10.1091/mbc.e04-11-1035;
RA Yorimitsu T., Klionsky D.J.;
RT "Atg11 links cargo to the vesicle-forming machinery in the cytoplasm to
RT vacuole targeting pathway.";
RL Mol. Biol. Cell 16:1593-1605(2005).
RN [9]
RP FUNCTION.
RX PubMed=15901835; DOI=10.1091/mbc.e04-10-0894;
RA Cheong H., Yorimitsu T., Reggiori F., Legakis J.E., Wang C.W.,
RA Klionsky D.J.;
RT "Atg17 regulates the magnitude of the autophagic response.";
RL Mol. Biol. Cell 16:3438-3453(2005).
RN [10]
RP FUNCTION.
RX PubMed=16138904; DOI=10.1111/j.1600-0854.2005.00327.x;
RA Berger A.C., Hanson P.K., Wylie Nichols J., Corbett A.H.;
RT "A yeast model system for functional analysis of the Niemann-Pick type C
RT protein 1 homolog, Ncr1p.";
RL Traffic 6:907-917(2005).
RN [11]
RP FUNCTION.
RX PubMed=17132049; DOI=10.1371/journal.pbio.0040423;
RA Bernales S., McDonald K.L., Walter P.;
RT "Autophagy counterbalances endoplasmic reticulum expansion during the
RT unfolded protein response.";
RL PLoS Biol. 4:E423-E423(2006).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [13]
RP FUNCTION.
RX PubMed=19793921; DOI=10.1091/mbc.e09-03-0225;
RA Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z.,
RA Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J.;
RT "A genomic screen for yeast mutants defective in selective mitochondria
RT autophagy.";
RL Mol. Biol. Cell 20:4730-4738(2009).
RN [14]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361 AND SER-363, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [15]
RP FUNCTION.
RX PubMed=20861302; DOI=10.1091/mbc.e10-05-0457;
RA Ohashi Y., Munro S.;
RT "Membrane delivery to the yeast autophagosome from the Golgi-endosomal
RT system.";
RL Mol. Biol. Cell 21:3998-4008(2010).
RN [16]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt), pexophagy
CC and mitophagy. Also involved in endoplasmic reticulum-specific
CC autophagic process and is essential for the survival of cells subjected
CC to severe ER stress. Functions in protein retrieval from the endocytic
CC pathway. Required for proper sorting of the v-SNARE protein SNC1.
CC {ECO:0000269|PubMed:12048214, ECO:0000269|PubMed:12554655,
CC ECO:0000269|PubMed:15901835, ECO:0000269|PubMed:16138904,
CC ECO:0000269|PubMed:17132049, ECO:0000269|PubMed:19793921,
CC ECO:0000269|PubMed:20861302}.
CC -!- SUBUNIT: Forms a complex with SNX4 and ATG17.
CC -!- INTERACTION:
CC Q07528; P47057: SNX4; NbExp=7; IntAct=EBI-36894, EBI-17610;
CC Q07528; P53039: YIP1; NbExp=2; IntAct=EBI-36894, EBI-25295;
CC -!- SUBCELLULAR LOCATION: Endosome membrane; Peripheral membrane protein.
CC Preautophagosomal structure membrane; Peripheral membrane protein.
CC -!- DOMAIN: The PX domain binds phosphatidylinositol 3-phosphate which is
CC necessary for peripheral membrane localization of ATG20 to the
CC perivacuolar punctate structures.
CC -!- MISCELLANEOUS: Present with 358 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the sorting nexin family. {ECO:0000305}.
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DR EMBL; Z74161; CAA98681.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11747.1; -; Genomic_DNA.
DR PIR; S67656; S67656.
DR RefSeq; NP_010170.1; NM_001180172.1.
DR AlphaFoldDB; Q07528; -.
DR SMR; Q07528; -.
DR BioGRID; 31949; 112.
DR ComplexPortal; CPX-1377; SNX4-ATG20 sorting nexin complex.
DR DIP; DIP-1593N; -.
DR IntAct; Q07528; 13.
DR MINT; Q07528; -.
DR STRING; 4932.YDL113C; -.
DR iPTMnet; Q07528; -.
DR MaxQB; Q07528; -.
DR PaxDb; Q07528; -.
DR PRIDE; Q07528; -.
DR EnsemblFungi; YDL113C_mRNA; YDL113C; YDL113C.
DR GeneID; 851445; -.
DR KEGG; sce:YDL113C; -.
DR SGD; S000002271; ATG20.
DR VEuPathDB; FungiDB:YDL113C; -.
DR eggNOG; KOG2273; Eukaryota.
DR HOGENOM; CLU_014456_2_1_1; -.
DR InParanoid; Q07528; -.
DR OMA; ICNTDIT; -.
DR BioCyc; YEAST:G3O-29513-MON; -.
DR PRO; PR:Q07528; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q07528; protein.
DR GO; GO:0010009; C:cytoplasmic side of endosome membrane; IDA:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0019898; C:extrinsic component of membrane; IDA:SGD.
DR GO; GO:0000407; C:phagophore assembly site; IDA:SGD.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
DR GO; GO:0000422; P:autophagy of mitochondrion; IMP:SGD.
DR GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IMP:SGD.
DR GO; GO:0034498; P:early endosome to Golgi transport; IMP:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IDA:ComplexPortal.
DR GO; GO:0016236; P:macroautophagy; IMP:SGD.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IDA:ComplexPortal.
DR CDD; cd06867; PX_SNX41_42; 1.
DR DisProt; DP01744; -.
DR Gene3D; 1.20.1270.60; -; 2.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR044106; PX_Snx41/Atg20.
DR Pfam; PF00787; PX; 1.
DR SMART; SM00312; PX; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50195; PX; 1.
PE 1: Evidence at protein level;
KW Acetylation; Autophagy; Coiled coil; Endosome; Lipid-binding; Membrane;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..640
FT /note="Autophagy-related protein 20"
FT /id="PRO_0000213824"
FT DOMAIN 140..301
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 126..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 475..512
FT /evidence="ECO:0000255"
FT COILED 562..593
FT /evidence="ECO:0000255"
FT COMPBIAS 1..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..152
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 192
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 194
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 218
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 267
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 361
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 363
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MUTAGEN 193
FT /note="Y->A: Abolishes the intracellular punctate
FT localization and decreases the cytoplasm to vacuole
FT transport."
FT /evidence="ECO:0000269|PubMed:12048214"
SQ SEQUENCE 640 AA; 72546 MW; 160977A8BECB6D93 CRC64;
MSDLNDVQEN AKLNSETRNT GKAEPPHGTT EYVAEAEISK NGVGSPKKSP KKGKVGKGDN
NKVETELVHT ALLEKDNPFM EEGPTGFTKS ALLEIPGMRS HNLKNPNEDY EDDSEGLLPL
NQESNAETCR TSLSGSINSM NGETSASEEP SVSNRKKSAR IHILEAKRVS EGQGRAYIAY
VIQFENSTVQ RRYSDFESLR SILIRLFPMT LIPPIPEKQS IKNYGKSITG SSSKYLLPSE
GSGSVDLSLS VIHASVNNSD EKLIRHRIRM LTEFLNKLLT NEEITKTSII TDFLDPNNHN
WHEFVNSSST FSSLPKSILQ CNPLDPTNTT RIHAMLPIPG SSSQLLLNKE SNDKKMDKER
SKSFTNIEQD YKQYENLLDN GIYKYNRRTT KTYHDLKSDY NEIGEVFAQF AHEQAQVGEL
AEQLSYLSNA FSGSSISLEK LVGRLYYNIN EPLNESVHMA TSARELIKYR KLKYLQNEMI
KKSLNSKRAQ LEKLEAQNNE YKDVDKIIDN EMSKSHTINL ERPNNNTGSG GKSYGGKLFN
GFNKLASMVK DSVKYQETDP HTASINLKKE IEQLSESLEV TENDLEVISK VIKNDQLPKF
SKEREVDLSE ILKHYSRYMR NYARQNLEIW KEVKRHQDFA