ID   ATG21_YEAS7             Reviewed;         496 AA.
AC   A6ZWF0;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Autophagy-related protein 21;
GN   Name=ATG21; ORFNames=SCY_5627;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) vesicles
CC       formation and mitophagy. Involved in binding of
CC       phosphatidylethanolamine to ATG8 and in recruitment of ATG8 and ATG5 to
CC       the pre-autophagosomal structure. Protects ATG8 from ARG4-mediated
CC       cleavage. Essential for maturation of proaminopeptidase I (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Vacuole membrane
CC       {ECO:0000250}. Note=And perivacuolar punctate structures.
CC       {ECO:0000250}.
CC   -!- DOMAIN: Contains a beta-propeller domain involved in specific binding
CC       to phosphatidylinositol 3,5-bisphosphate (PIP2).
CC   -!- DOMAIN: The L/FRRG motif is essential for the cytoplasm to vacuole
CC       transport (Cvt) pathway and for the recruitment of ATG8 and ATG16 to
CC       the PAS in nutrient-rich medium and in both its recruitment to and
CC       dissociation from the PAS under starvation conditions.
CC       {ECO:0000250|UniProtKB:Q02887}.
CC   -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR   EMBL; AAFW02000135; EDN61042.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZWF0; -.
DR   SMR; A6ZWF0; -.
DR   EnsemblFungi; EDN61042; EDN61042; SCY_5627.
DR   HOGENOM; CLU_025895_5_2_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   3: Inferred from homology;
KW   Autophagy; Cytoplasm; Membrane; Phosphoprotein; Protein transport; Repeat;
KW   Transport; Vacuole; WD repeat.
FT   CHAIN           1..496
FT                   /note="Autophagy-related protein 21"
FT                   /id="PRO_0000318013"
FT   REPEAT          1..35
FT                   /note="WD 1"
FT   REPEAT          148..190
FT                   /note="WD 2"
FT   REPEAT          294..334
FT                   /note="WD 3"
FT   REPEAT          346..385
FT                   /note="WD 4"
FT   REPEAT          448..488
FT                   /note="WD 5"
FT   REGION          41..86
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           342..346
FT                   /note="L/FRRG motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q02887"
FT   COMPBIAS        43..70
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         213
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q02887"
FT   MOD_RES         237
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q02887"
SQ   SEQUENCE   496 AA;  55138 MW;  4EB31964F6437CEB CRC64;
     MKVLQFNQDA TCCVVAASSH QISIFNCDPF GKCFEIDTKN SKKKTSNNNG TASNSESRNN
     EESILITNGS RDRTDAEEEE DNEDNALVTG NILKEGEFVI EMLFSTSLIA IADRGQGLNK
     GKKLKIVNTK RKSTICEIVF PHEIVDVVMN RKRMCVLLES DQIFIYDISC MKPLETIDLW
     EDHYKRSQAN SFSNASNTGT LEGDSANLNR VATNLLANAT QKSVNGSNPS VRTRRNSLRS
     KIRPRMVLSN DDRSILCFTA YSSPKKNKPN SEALYDVVIY DTLNVTPVNY LNSVHKGNVA
     CLAVSHDGKL LATASDKGTI IRVFHTGVDS DYMSSRSLFK EFRRGTRLCN LYQLAFDKSM
     TMIGCVGDTD TIHLFKLDDA SNSLPGDNSS NGHWNEEEDI LASNSNPSMG TPKEIPLSKP
     RIANYFSKKI KSSIPNQNLS RNFAYITVNE SNRSCLGFPD EFPNQVYIAS DDGTFSIYSI
     PSKPGECVLT KNNKFT