ID   ATG25_PICAN             Reviewed;         384 AA.
AC   Q6JUT9;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Autophagy-related protein 25;
DE   AltName: Full=Peroxisome degradation deficient protein 4;
GN   Name=ATG25; Synonyms=PDD4;
OS   Pichia angusta (Yeast) (Hansenula polymorpha).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Pichiaceae; Ogataea.
OX   NCBI_TaxID=870730;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC   Y-5445;
RA   Monastyrska I., Kiel J.A.K.W., Veenhuis M.;
RT   "The H. polymorpha PDD4 gene product is required for selective peroxisome
RT   degradation.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RX   PubMed=11810237; DOI=10.1007/s004380100584;
RA   van Dijk R., Faber K.N., Hammond A.T., Glick B.S., Veenhuis M.,
RA   Kiel J.A.K.W.;
RT   "Tagging Hansenula polymorpha genes by random integration of linear DNA
RT   fragments (RALF).";
RL   Mol. Genet. Genomics 266:646-656(2001).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=14536056; DOI=10.1016/s1534-5807(03)00296-x;
RA   Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y.,
RA   Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.;
RT   "A unified nomenclature for yeast autophagy-related genes.";
RL   Dev. Cell 5:539-545(2003).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=16874036; DOI=10.4161/auto.1.2.1832;
RA   Monastyrska I., Kiel J.A., Krikken A.M., Komduur J.A., Veenhuis M.,
RA   van der Klei I.J.;
RT   "The Hansenula polymorpha ATG25 gene encodes a novel coiled-coil protein
RT   that is required for macropexophagy.";
RL   Autophagy 1:92-100(2005).
CC   -!- FUNCTION: Specifically required for selective degradation of
CC       peroxisomes via macropexophagy. {ECO:0000269|PubMed:11810237,
CC       ECO:0000269|PubMed:16874036}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000269|PubMed:16874036}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:16874036}; Cytoplasmic side
CC       {ECO:0000269|PubMed:16874036}.
CC   -!- SIMILARITY: Belongs to the ADIP family. {ECO:0000305}.
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DR   EMBL; AY304543; AAQ74772.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6JUT9; -.
DR   SMR; Q6JUT9; -.
DR   PRIDE; Q6JUT9; -.
DR   PhylomeDB; Q6JUT9; -.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR021622; Afadin/alpha-actinin-bd.
DR   Pfam; PF11559; ADIP; 1.
PE   3: Inferred from homology;
KW   Autophagy; Coiled coil; Membrane; Protein transport; Transport.
FT   CHAIN           1..384
FT                   /note="Autophagy-related protein 25"
FT                   /id="PRO_0000064728"
FT   REGION          224..247
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          132..236
FT                   /evidence="ECO:0000255"
FT   COILED          342..379
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   384 AA;  45252 MW;  E294618BBCE6AC12 CRC64;
     MFFFGDASTR PQIKFLCLFI YFIYMEEIFE KSQLENEQPT SKEPEPALKP VDVMSIKNAA
     QYVNQKLSAK GYFSNKPNKL RQLLLLSLDS SQLIPENAEN FEISEKVYEN DRNVMNIIYS
     LLNSAEISKQ FKETALKKMA DKDAEIELLK NEVEKLNKKV DEKERNIQSL QLDKIKSDLQ
     AKHYQAMLNN VNAKNKEQER TFRLYAEEVK RELRRNEMEV DRLESRLSNM NKRRASPRDD
     AEAEPKRQKF DNDRFLSLVK ENSHLQHDLN KLVGVLLRLQ KFLKSFGQLN SNSKVPSTFI
     PTDSELLAID HHNFDLQKYD LILFDLLKPL NGDSIDDTFK FKKNNQTDKD QEIASLRNRL
     SEMEQNYERV LATMEQWKTY RKQK