ATG26_VANPO
ID ATG26_VANPO Reviewed; 1217 AA.
AC A7TF84;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Sterol 3-beta-glucosyltransferase {ECO:0000305};
DE EC=2.4.1.173 {ECO:0000305};
DE AltName: Full=Autophagy-related protein 26 {ECO:0000250|UniProtKB:Q06321};
GN Name=ATG26 {ECO:0000250|UniProtKB:Q06321}; ORFNames=Kpol_2000p77;
OS Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX NCBI_TaxID=436907;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC Y-8283 / UCD 57-17;
RX PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT species descended from a whole-genome duplication.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC -!- FUNCTION: Probable sterol 3-beta-glucosyltransferase that is not
CC involved in cytoplasm to vacuole transport (Cvt), pexophagy or
CC nonselective autophagy (By similarity). {ECO:0000250|UniProtKB:Q06321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside +
CC H(+) + UDP; Xref=Rhea:RHEA:22724, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15889, ChEBI:CHEBI:37424, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.173; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q06321}.
CC Membrane {ECO:0000250|UniProtKB:Q06321}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q06321}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC {ECO:0000305}.
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DR EMBL; DS480382; EDO19109.1; -; Genomic_DNA.
DR RefSeq; XP_001646967.1; XM_001646917.1.
DR AlphaFoldDB; A7TF84; -.
DR SMR; A7TF84; -.
DR STRING; 436907.A7TF84; -.
DR EnsemblFungi; EDO19109; EDO19109; Kpol_2000p77.
DR GeneID; 5547438; -.
DR KEGG; vpo:Kpol_2000p77; -.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_000537_6_0_1; -.
DR InParanoid; A7TF84; -.
DR OMA; WCNNITK; -.
DR OrthoDB; 1024049at2759; -.
DR PhylomeDB; A7TF84; -.
DR Proteomes; UP000000267; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0102203; F:brassicasterol glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102205; F:cholesterol alpha-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102202; F:soladodine glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016906; F:sterol 3-beta-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0030259; P:lipid glycosylation; IEA:InterPro.
DR GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR004276; GlycoTrans_28_N.
DR InterPro; IPR004182; GRAM.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR002213; UDP_glucos_trans.
DR Pfam; PF03033; Glyco_transf_28; 1.
DR Pfam; PF02893; GRAM; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00201; UDPGT; 1.
DR SMART; SM00568; GRAM; 2.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Glycosyltransferase; Lipid biosynthesis; Lipid metabolism;
KW Membrane; Reference proteome; Repeat; Steroid biosynthesis;
KW Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase.
FT CHAIN 1..1217
FT /note="Sterol 3-beta-glucosyltransferase"
FT /id="PRO_0000318049"
FT DOMAIN 195..232
FT /note="GRAM 1"
FT /evidence="ECO:0000255"
FT DOMAIN 246..343
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 590..656
FT /note="GRAM 2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1217 AA; 138412 MW; 57C553A3F01C3653 CRC64;
MIRNSTNTNN IMETEVALKD WILYYHKLCE VNSGEIFSGN EIITDFDDPV LSDPLLNLKP
LPKNEVTVNK DNVIKDNDYS SNGCDFAKSK FHIKGLVGLL TTASVYIGLD NSTNPSYDIS
QACEFDLEDN TVQQGTGRSA NMNPACKVAG FNKSSVRDTI FNIFKIPKKD EKISNDMEVN
NTLSRDLDYK KDLFEFVRKY FGISEEETLI GHYTGWLLQE VLIQGNLFIT NSSLVYLAHL
PKLTDAVVLC GKLKLRSRLK GNPRYWCVLK HSTLALYNDP SDIYFPILSI DLNHVEEISL
ENSLKDENTM TFVLEASSKN YKFIAGSSHS AKTWVKCLKK QLFSIKNQSK DTIGIKFPIS
SIIDVECQSY MNQGQSIKVK CFDGNKNYAL KEYTFLFFDK DGDSFYNIIS NFIPRNMNPV
EASLQNSNPT IHLNSKSHFN DKIKILSAVN IDDIICDTDS FEMTSSDILS DIDENIQPLS
LKHVRDISNC KNYLKEDLKT SNKMTIQDCN KKVQTSEIQL ITYSKHDSRM ELNDDSKYYQ
GNIGNDNKGI LSFKNICSIW NTSPIHNKDS DNFFMTSDPF VTSINDTTLA KIKDWFNLHD
NEVLHALYYA YLIKGYPVYG KLYVTNRRLY FKSCIPGVNV KMVLPLEDIE GYNEILGTNY
GNFGILLTVQ NEKELQFGFN SCTNRSDFEN VLQRCLDICK YAIKTPELVT SRVIESESEH
SRLRFFEEKF STEGIDIPFL VEDNPYFKTK IMPTKSYNFG FLTIGSRGDV QPYIALAKGL
IQEGHSVTII THREFKSFVE CHGIDFKEIA GDPTKLMSLM VEHEAINVGM LMEASSKFRG
WIHDLLVTTW EACKNLKLDI LIESPSAMAG IHISEALQIP YFRAFTMPWT RTRAYPHAFI
VPDQKRGGSF NYLTHVIFEN VFWRGICSQV NKWRVQTLGL EKTNLAQLQQ NKIPFLYNIS
PVIFPPAIDF DEWIKVTGYW FLDESESFEP SQELETFISK ARKLGKKLVY IGFGSIVVNN
AKEMTRAVID SVLETDIFCI LNKGWSERLG KEELRYEEEP EYPETIFLCD SIPHDWLFPK
VDAAVHHGGS GTTGATLKAG TPVVIKPFFG DQFFFASRIE DIGAGIALKK LNVSSLSNAI
KKVLTDKSIK RKAVSLKKRV AKENGVTTAI NCIYSELEYA RSLVVKKNHK SSNIEFIQHP
NNVNDTTKTV IPLTSMV
