ID   PROF1_CITSI             Reviewed;         131 AA.
AC   P84177; Q5TIM0;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 2.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Profilin {ECO:0000303|PubMed:16197476};
DE   AltName: Allergen=Cit s 2 {ECO:0000303|PubMed:16197476};
OS   Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=2711;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-11, MASS SPECTROMETRY,
RP   ALLERGEN, AND VARIANTS PHE-60; THR-93 AND ARG-114.
RC   STRAIN=cv. Navel late {ECO:0000269|PubMed:16197476};
RC   TISSUE=Fruit flesh {ECO:0000269|PubMed:16197476}, and
RC   Peelings {ECO:0000269|PubMed:16197476};
RX   PubMed=16197476; DOI=10.1111/j.1398-9995.2005.00903.x;
RA   Lopez-Torrejon G., Ibanez M.D., Ahrazem O., Sanchez-Monge R., Sastre J.,
RA   Lombardero M., Barber D., Salcedo G.;
RT   "Isolation, cloning and allergenic reactivity of natural profilin Cit s 2,
RT   a major orange allergen.";
RL   Allergy 60:1424-1429(2005).
RN   [2]
RP   ALLERGEN.
RX   PubMed=16521162; DOI=10.1002/mnfr.200500200;
RA   Crespo J.F., Retzek M., Foetisch K., Sierra-Maestro E., Cid-Sanchez A.B.,
RA   Pascual C.Y., Conti A., Feliu A., Rodriguez J., Vieths S., Scheurer S.;
RT   "Germin-like protein Cit s 1 and profilin Cit s 2 are major allergens in
RT   orange (Citrus sinensis) fruits.";
RL   Mol. Nutr. Food Res. 50:282-290(2006).
CC   -!- FUNCTION: Binds to actin and affects the structure of the cytoskeleton.
CC       At high concentrations, profilin prevents the polymerization of actin,
CC       whereas it enhances it at low concentrations. By binding to PIP2, it
CC       inhibits the formation of IP3 and DG (By similarity).
CC       {ECO:0000250|UniProtKB:P49233}.
CC   -!- SUBUNIT: Occurs in many kinds of cells as a complex with monomeric
CC       actin in a 1:1 ratio. {ECO:0000250|UniProtKB:P49233}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:P49233}.
CC   -!- MASS SPECTROMETRY: Mass=14137; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16197476};
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE. Induces
CC       histamine release from basophils of profilin-allergic patients.
CC       {ECO:0000269|PubMed:16197476, ECO:0000269|PubMed:16521162}.
CC   -!- SIMILARITY: Belongs to the profilin family. {ECO:0000305}.
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DR   EMBL; AJ865015; CAI23765.1; -; mRNA.
DR   AlphaFoldDB; P84177; -.
DR   SMR; P84177; -.
DR   Allergome; 1173; Cit s 2.
DR   Allergome; 3200; Cit s 2.0101.
DR   PRIDE; P84177; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   CDD; cd00148; PROF; 1.
DR   InterPro; IPR005455; PFN.
DR   InterPro; IPR036140; PFN_sf.
DR   InterPro; IPR027310; Profilin_CS.
DR   PANTHER; PTHR11604; PTHR11604; 1.
DR   Pfam; PF00235; Profilin; 1.
DR   PRINTS; PR00392; PROFILIN.
DR   PRINTS; PR01640; PROFILINPLNT.
DR   SMART; SM00392; PROF; 1.
DR   SUPFAM; SSF55770; SSF55770; 1.
DR   PROSITE; PS00414; PROFILIN; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Allergen; Cytoplasm; Cytoskeleton;
KW   Direct protein sequencing.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:16197476"
FT   CHAIN           2..131
FT                   /note="Profilin"
FT                   /evidence="ECO:0000269|PubMed:16197476"
FT                   /id="PRO_0000199626"
FT   VARIANT         60
FT                   /note="L -> F"
FT                   /evidence="ECO:0000269|PubMed:16197476"
FT   VARIANT         93
FT                   /note="I -> T"
FT                   /evidence="ECO:0000269|PubMed:16197476"
FT   VARIANT         114
FT                   /note="Q -> R"
FT                   /evidence="ECO:0000269|PubMed:16197476"
SQ   SEQUENCE   131 AA;  14060 MW;  163FC690C9444866 CRC64;
     MSWQAYVDDH LMCDIDGNRL TAAAILGQDG SVWSQSATFP AFRLEEIAAI LKDFDQPGTL
     APTGLFLGGT KYMVIQGEAG AVIRGKKGSG GIIVKKTNQA LIIGIYDEPL TPGQCNMIVE
     RLGDYLIEQG L