PROF4_HUMAN
ID PROF4_HUMAN Reviewed; 129 AA.
AC Q8NHR9; Q53TL9;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Profilin-4;
DE AltName: Full=Profilin IV;
GN Name=PFN4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=15591451; DOI=10.1093/molehr/gah132;
RA Obermann H., Raabe I., Balvers M., Brunswig B., Schulze W., Kirchhoff C.;
RT "Novel testis-expressed profilin IV associated with acrosome biogenesis and
RT spermatid elongation.";
RL Mol. Hum. Reprod. 11:53-64(2005).
RN [6]
RP FUNCTION.
RX PubMed=19419568; DOI=10.1186/1471-2121-10-34;
RA Behnen M., Murk K., Kursula P., Cappallo-Obermann H., Rothkegel M.,
RA Kierszenbaum A.L., Kirchhoff C.;
RT "Testis-expressed profilins 3 and 4 show distinct functional
RT characteristics and localize in the acroplaxome-manchette complex in
RT spermatids.";
RL BMC Cell Biol. 10:34-34(2009).
CC -!- FUNCTION: Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P),
CC phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2),
CC phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidic acid
CC (PA). Does not bind to actin (PubMed:19419568), contrary to other
CC family members. {ECO:0000269|PubMed:19419568}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Detected in testis (at protein level).
CC {ECO:0000269|PubMed:15591451}.
CC -!- INDUCTION: Reduced levels in Sertoli cell-only syndrome (SCOS)
CC patients. {ECO:0000269|PubMed:15591451}.
CC -!- SIMILARITY: Belongs to the profilin family. {ECO:0000305}.
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DR EMBL; AK292601; BAF85290.1; -; mRNA.
DR EMBL; AC008073; AAY14666.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00760.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00761.1; -; Genomic_DNA.
DR EMBL; BC029523; AAH29523.1; -; mRNA.
DR CCDS; CCDS1709.1; -.
DR RefSeq; NP_955378.1; NM_199346.2.
DR RefSeq; XP_005264349.1; XM_005264292.2.
DR RefSeq; XP_006712070.1; XM_006712007.3.
DR RefSeq; XP_011531125.1; XM_011532823.2.
DR AlphaFoldDB; Q8NHR9; -.
DR SMR; Q8NHR9; -.
DR STRING; 9606.ENSP00000322170; -.
DR iPTMnet; Q8NHR9; -.
DR PhosphoSitePlus; Q8NHR9; -.
DR BioMuta; PFN4; -.
DR DMDM; 48474487; -.
DR MassIVE; Q8NHR9; -.
DR PaxDb; Q8NHR9; -.
DR PeptideAtlas; Q8NHR9; -.
DR PRIDE; Q8NHR9; -.
DR ProteomicsDB; 73743; -.
DR Antibodypedia; 27446; 48 antibodies from 15 providers.
DR DNASU; 375189; -.
DR Ensembl; ENST00000313213.5; ENSP00000322170.4; ENSG00000176732.7.
DR GeneID; 375189; -.
DR KEGG; hsa:375189; -.
DR MANE-Select; ENST00000313213.5; ENSP00000322170.4; NM_199346.3; NP_955378.1.
DR UCSC; uc002rfa.2; human.
DR CTD; 375189; -.
DR GeneCards; PFN4; -.
DR HGNC; HGNC:31103; PFN4.
DR HPA; ENSG00000176732; Tissue enriched (testis).
DR neXtProt; NX_Q8NHR9; -.
DR OpenTargets; ENSG00000176732; -.
DR PharmGKB; PA134919643; -.
DR VEuPathDB; HostDB:ENSG00000176732; -.
DR eggNOG; KOG1755; Eukaryota.
DR GeneTree; ENSGT00390000017067; -.
DR HOGENOM; CLU_120772_2_0_1; -.
DR InParanoid; Q8NHR9; -.
DR OMA; VMPSDVQ; -.
DR PhylomeDB; Q8NHR9; -.
DR TreeFam; TF101075; -.
DR PathwayCommons; Q8NHR9; -.
DR BioGRID-ORCS; 375189; 7 hits in 1066 CRISPR screens.
DR GenomeRNAi; 375189; -.
DR Pharos; Q8NHR9; Tdark.
DR PRO; PR:Q8NHR9; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8NHR9; protein.
DR Bgee; ENSG00000176732; Expressed in left testis and 99 other tissues.
DR ExpressionAtlas; Q8NHR9; baseline and differential.
DR Genevisible; Q8NHR9; HS.
DR GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0003785; F:actin monomer binding; IBA:GO_Central.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0042989; P:sequestering of actin monomers; IBA:GO_Central.
DR CDD; cd00148; PROF; 1.
DR InterPro; IPR005455; PFN.
DR InterPro; IPR036140; PFN_sf.
DR PANTHER; PTHR11604; PTHR11604; 1.
DR Pfam; PF00235; Profilin; 1.
DR PRINTS; PR00392; PROFILIN.
DR SMART; SM00392; PROF; 1.
DR SUPFAM; SSF55770; SSF55770; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Lipid-binding; Reference proteome.
FT CHAIN 1..129
FT /note="Profilin-4"
FT /id="PRO_0000199581"
SQ SEQUENCE 129 AA; 14319 MW; 2BB7C78E66C0EAEF CRC64;
MSHLQSLLLD TLLGTKHVDS AALIKIQERS LCVASPGFNV TPSDVRTLVN GFAKNPLQAR
REGLYFKGKD YRCVRADEYS LYAKNENTGV VVVKTHLYLL VATYTEGMYP SICVEATESL
GDYLRKKGS
