ID   PROF4_RAT               Reviewed;         129 AA.
AC   Q5IRJ7;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Profilin-4;
DE   AltName: Full=Profilin IV;
GN   Name=Pfn4;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=Wistar; TISSUE=Testis;
RX   PubMed=15591451; DOI=10.1093/molehr/gah132;
RA   Obermann H., Raabe I., Balvers M., Brunswig B., Schulze W., Kirchhoff C.;
RT   "Novel testis-expressed profilin IV associated with acrosome biogenesis and
RT   spermatid elongation.";
RL   Mol. Hum. Reprod. 11:53-64(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX   PubMed=19419568; DOI=10.1186/1471-2121-10-34;
RA   Behnen M., Murk K., Kursula P., Cappallo-Obermann H., Rothkegel M.,
RA   Kierszenbaum A.L., Kirchhoff C.;
RT   "Testis-expressed profilins 3 and 4 show distinct functional
RT   characteristics and localize in the acroplaxome-manchette complex in
RT   spermatids.";
RL   BMC Cell Biol. 10:34-34(2009).
CC   -!- FUNCTION: Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P),
CC       phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2),
CC       phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidic acid
CC       (PA). Does not bind to actin, contrary to other family members (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC       Note=Localizes first in the acroplaxome and later in the manchette
CC       during spermatid head development. Localized mainly in the developing
CC       acrosome of haploid germ cells. {ECO:0000269|PubMed:15591451,
CC       ECO:0000269|PubMed:19419568}.
CC   -!- TISSUE SPECIFICITY: Detected in seminiferous tubules (at protein
CC       level). Detected in spermatocytes and spermatids. Not detected in
CC       spermatogonium. {ECO:0000269|PubMed:15591451}.
CC   -!- DEVELOPMENTAL STAGE: Expression coincides with the onset of meiosis.
CC       Detected in nearly all stages of spermiogenesis (at protein level).
CC       {ECO:0000269|PubMed:15591451, ECO:0000269|PubMed:19419568}.
CC   -!- SIMILARITY: Belongs to the profilin family. {ECO:0000305}.
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DR   EMBL; AY682392; AAV85168.1; -; mRNA.
DR   EMBL; AABR06042656; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH473947; EDM03047.1; -; Genomic_DNA.
DR   EMBL; BC107465; AAI07466.1; -; mRNA.
DR   RefSeq; NP_001009503.1; NM_001009503.1.
DR   AlphaFoldDB; Q5IRJ7; -.
DR   SMR; Q5IRJ7; -.
DR   STRING; 10116.ENSRNOP00000064666; -.
DR   PaxDb; Q5IRJ7; -.
DR   PRIDE; Q5IRJ7; -.
DR   Ensembl; ENSRNOT00000074570; ENSRNOP00000064666; ENSRNOG00000050386.
DR   GeneID; 494222; -.
DR   KEGG; rno:494222; -.
DR   CTD; 375189; -.
DR   RGD; 1359707; Pfn4.
DR   eggNOG; KOG1755; Eukaryota.
DR   GeneTree; ENSGT00390000017067; -.
DR   HOGENOM; CLU_120772_2_0_1; -.
DR   InParanoid; Q5IRJ7; -.
DR   OMA; VMPSDVQ; -.
DR   OrthoDB; 1428600at2759; -.
DR   PhylomeDB; Q5IRJ7; -.
DR   PRO; PR:Q5IRJ7; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Proteomes; UP000234681; Chromosome 6.
DR   Bgee; ENSRNOG00000050386; Expressed in testis.
DR   GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0003785; F:actin monomer binding; IBA:GO_Central.
DR   GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR   GO; GO:0042989; P:sequestering of actin monomers; IBA:GO_Central.
DR   CDD; cd00148; PROF; 1.
DR   InterPro; IPR005455; PFN.
DR   InterPro; IPR036140; PFN_sf.
DR   PANTHER; PTHR11604; PTHR11604; 1.
DR   Pfam; PF00235; Profilin; 1.
DR   SMART; SM00392; PROF; 1.
DR   SUPFAM; SSF55770; SSF55770; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoskeleton; Lipid-binding; Reference proteome.
FT   CHAIN           1..129
FT                   /note="Profilin-4"
FT                   /id="PRO_0000423634"
SQ   SEQUENCE   129 AA;  14421 MW;  44F9C0BC2D2D8E0A CRC64;
     MSHLQNLLLD TLLGTKHVDG AALIKLQEKT LCVTSPGFSV MPCDVRTLLN GFAKNPLLTR
     REGLYFREKD YKCVRADDCS LYAKKENTGV VVVKTHMYLL VATYTAGMYP SVCVEATEKL
     GEYLRKKGN