ID   ATG27_VANPO             Reviewed;         262 AA.
AC   A7TFH9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Autophagy-related protein 27;
DE   Flags: Precursor;
GN   Name=ATG27; ORFNames=Kpol_2002p64;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS   2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC   Y-8283 / UCD 57-17;
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT   species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: Regulates the cytoplasm to vacuole transport (Cvt) vesicle
CC       formation. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Golgi apparatus
CC       membrane {ECO:0000250}; Single-pass type I membrane protein
CC       {ECO:0000250}. Mitochondrion membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}. Note=Cycles among the pre-
CC       autophagosomal structure (PAS), mitochondria and Golgi. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG27 family. {ECO:0000305}.
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DR   EMBL; DS480383; EDO18993.1; -; Genomic_DNA.
DR   RefSeq; XP_001646851.1; XM_001646801.1.
DR   AlphaFoldDB; A7TFH9; -.
DR   STRING; 436907.A7TFH9; -.
DR   EnsemblFungi; EDO18993; EDO18993; Kpol_2002p64.
DR   GeneID; 5547319; -.
DR   KEGG; vpo:Kpol_2002p64; -.
DR   eggNOG; ENOG502QVJJ; Eukaryota.
DR   HOGENOM; CLU_089705_0_0_1; -.
DR   InParanoid; A7TFH9; -.
DR   OMA; NKGNAID; -.
DR   OrthoDB; 1240091at2759; -.
DR   PhylomeDB; A7TFH9; -.
DR   Proteomes; UP000000267; Unassembled WGS sequence.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR018939; Autophagy-rel_prot_27.
DR   InterPro; IPR044865; MRH_dom.
DR   Pfam; PF09451; ATG27; 1.
DR   PROSITE; PS51914; MRH; 1.
PE   3: Inferred from homology;
KW   Autophagy; Cytoplasmic vesicle; Disulfide bond; Golgi apparatus; Membrane;
KW   Mitochondrion; Protein transport; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..262
FT                   /note="Autophagy-related protein 27"
FT                   /id="PRO_0000318055"
FT   TOPO_DOM        21..185
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        207..262
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   DOMAIN          21..170
FT                   /note="MRH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
FT   DISULFID        23..61
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
FT   DISULFID        73..80
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
FT   DISULFID        139..168
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01262"
SQ   SEQUENCE   262 AA;  29436 MW;  A7C72573A1D844DD CRC64;
     MLKMRLLLTW VLLVLPLVNA LKCANNRVLR KYHIDKHSAK KSVARDTPPS KTTEKWYVNP
     CEEHPADDIY DGCDKSDDIC GIVMVDLPVF NKDPFVIKRI EATDMASFTA TEESNALVLR
     YTGISWGQNM VAANIRYFCD KNSNEDEITS SIWANNDISI IIKGPSGCKK DSSELEDGDV
     EESSGLSWFT WLFIYAIFFT VVYLVVTSYT QTRGGSIDDF RHDFVERAKQ FFTSLPAFVR
     EVVSKVLGSA PNAAERGGYS AV