ID   ATG28_GIBZE             Reviewed;         646 AA.
AC   A0A098DT87; A0A0E0SF68;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   07-JAN-2015, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Autophagy-related protein 28 {ECO:0000303|PubMed:28894236};
GN   Name=ATG28 {ECO:0000303|PubMed:28894236};
GN   ORFNames=FG07526, FGRAMPH1_01T25019;
OS   Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS   / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=229533;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=17823352; DOI=10.1126/science.1143708;
RA   Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA   Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA   Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA   Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA   Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA   Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA   Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA   Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT   "The Fusarium graminearum genome reveals a link between localized
RT   polymorphism and pathogen specialization.";
RL   Science 317:1400-1402(2007).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX   PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA   King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA   Hammond-Kosack K.E.;
RT   "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT   graminearum.";
RL   BMC Genomics 16:544-544(2015).
RN   [4]
RP   IDENTIFICATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=28894236; DOI=10.1038/s41598-017-11640-z;
RA   Lv W., Wang C., Yang N., Que Y., Talbot N.J., Wang Z.;
RT   "Genome-wide functional analysis reveals that autophagy is necessary for
RT   growth, sporulation, deoxynivalenol production and virulence in Fusarium
RT   graminearum.";
RL   Sci. Rep. 7:11062-11062(2017).
CC   -!- FUNCTION: Required for the autophagic degradation of peroxisomes called
CC       pexophagy, but not essential for general autophagy (By similarity).
CC       Involved in resistance to elevated pH (By similarity).
CC       {ECO:0000250|UniProtKB:C4R159}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:C4R159}. Vacuole
CC       membrane {ECO:0000250|UniProtKB:C4R159}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:C4R159}. Cytoplasmic vesicle membrane
CC       {ECO:0000250|UniProtKB:C4R159}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:C4R159}. Note=Concentration increases at the
CC       vacuolar and cytoplasmic vesicular membranes during the course of
CC       pexophagy (By similarity). {ECO:0000250|UniProtKB:C4R159}.
CC   -!- DISRUPTION PHENOTYPE: Does not significantly decrease the growth rate
CC       under nutrient-rich conditions (PubMed:28894236).
CC       {ECO:0000269|PubMed:28894236}.
CC   -!- SIMILARITY: Belongs to the ATG28 family. {ECO:0000305}.
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DR   EMBL; HG970335; CEF85081.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A098DT87; -.
DR   SMR; A0A098DT87; -.
DR   STRING; 229533.A0A098DT87; -.
DR   VEuPathDB; FungiDB:FGRAMPH1_01G25019; -.
DR   eggNOG; ENOG502SANW; Eukaryota.
DR   Proteomes; UP000070720; Chromosome 4.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Autophagy; Coiled coil; Cytoplasm; Cytoplasmic vesicle; Membrane;
KW   Reference proteome; Vacuole.
FT   CHAIN           1..646
FT                   /note="Autophagy-related protein 28"
FT                   /id="PRO_0000443862"
FT   REGION          1..148
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          221..245
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          475..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          546..612
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          283..350
FT                   /evidence="ECO:0000255"
FT   COILED          485..514
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        28..50
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        59..90
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        106..123
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        479..494
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        555..598
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   646 AA;  72220 MW;  754DC7D4977A6463 CRC64;
     MSSPSMFDRI SSPRQRLSNP LRSPPIREVS EYDLDELEPR SDDVLFDQEP PLPRLKKNMQ
     HVSATSTRRS SSPASWDSKH RYSNSPPHTR SKPIFAGPPP PIASSMMMNQ HPSRQSTVSS
     HGDNGRGYGL ISASRFGSNS PSQKHVDNRP RLDSIWRSLQ RREKALERDI QQLLDLQASG
     LIAGSGEGSE SNFGSDTTTG ESTFYSTATS KSRMMNSLHM PTRSTPDGNV IPVRQPVSNK
     PRGLKSTRVG LQRSMAALSD LKAEEDLHLS TALEQRKDAL AYLDKMSKRR DDIYSELHAL
     EDDEEEPLGQ ELRSLEAERQ ELDHDIQRLE EKLAGAKKRR RWVREKMEDV KGRREAGLSG
     YRAAGRDVDI EVRTLMQTPP IAPLDIDALG YGENTRPKEN MDILRGTEFL QLRPERRTVE
     MARTWWQGEI AALERRKAQI SQDRQALIEG SEVWSDVTGL VAQFEAKLRE LVKSSQAGDA
     DEEPQQAAMQ SQLSEMDDVV QELQKRLQLA ESKHWNLLIC AIGAELEAFV EAKALLSDTL
     GLPEPAAAVD SPELSDSTDK AEGTSQEERA DSHDESDNEV PADLLVSRME DHDHDPPDSP
     QQQSVVLRRG SAGNNEVPVE FLADLHGPNK IEQDTEIAAV TRLMSK