ID   PROH2_APIME             Reviewed;         351 AA.
AC   P85799;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Prohormone-2;
DE   Contains:
DE     RecName: Full=Brain peptide NVPIYQEPRF;
DE   Contains:
DE     RecName: Full=Brain peptide VPIYQEPRF;
DE   Contains:
DE     RecName: Full=Brain peptide SQAYDPYSNAAQFQLSSQSRGYPYQHRLVY;
DE   Contains:
DE     RecName: Full=Brain peptide GYPYQHRLVY;
DE   Flags: Precursor;
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DH4;
RG   Honey bee genome project;
RA   Zhang L., Deng J., Wu Y.-Q., Kovar C., Aqrawi P., Bandaranaike D.,
RA   Blankenburg K., Chen D., Denson S., Dinh H., Firestine M., Gross S.,
RA   Han Y., Hernandez B., Holder M., Jackson L., Javaid M., Jing C., Jones J.,
RA   Joshi V., Kamau G., Korchina V., Lee S., Lorensuhewa L., Mata R.,
RA   Mathew T., Mims S., Ngo R., Nguyen L., Okwuonu G., Ongeri F., Osuji N.,
RA   Pham C., Puazo M., Qu C., Quiroz J., Raj R., Rio Deiros D., Santibanez J.,
RA   Scheel M., Scherer S., Vee V., Wang M., Xin Y., Richards S., Reid J.G.,
RA   Newsham I., Worley K.C., Muzny D.M., Gibbs R.;
RL   Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 180-189 AND 322-351, AND MASS SPECTROMETRY.
RC   TISSUE=Brain {ECO:0000269|PubMed:17068263};
RX   PubMed=17068263; DOI=10.1126/science.1124128;
RA   Hummon A.B., Richmond T.A., Verleyen P., Baggerman G., Huybrechts J.,
RA   Ewing M.A., Vierstraete E., Rodriguez-Zas S.L., Schoofs L., Robinson G.E.,
RA   Sweedler J.V.;
RT   "From the genome to the proteome: uncovering peptides in the Apis brain.";
RL   Science 314:647-649(2006).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: [Brain peptide NVPIYQEPRF]: Mass=1261.65;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC   -!- MASS SPECTROMETRY: [Brain peptide VPIYQEPRF]: Mass=1147.6;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC   -!- MASS SPECTROMETRY: [Brain peptide SQAYDPYSNAAQFQLSSQSRGYPYQHRLVY]:
CC       Mass=3523.56; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:17068263};
CC   -!- MASS SPECTROMETRY: [Brain peptide GYPYQHRLVY]: Mass=1294.65;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
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DR   EMBL; AADG06001790; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001161308.1; NM_001167836.1.
DR   AlphaFoldDB; P85799; -.
DR   STRING; 7460.GB44988-PA; -.
DR   PaxDb; P85799; -.
DR   EnsemblMetazoa; NM_001167836; NP_001161308; LOC409314.
DR   GeneID; 409314; -.
DR   KEGG; ame:409314; -.
DR   eggNOG; ENOG502SEH3; Eukaryota.
DR   HOGENOM; CLU_067916_0_0_1; -.
DR   InParanoid; P85799; -.
DR   OMA; YQEPRYK; -.
DR   OrthoDB; 1355683at2759; -.
DR   PhylomeDB; P85799; -.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..177
FT                   /evidence="ECO:0000269|PubMed:17068263"
FT                   /id="PRO_0000341470"
FT   PEPTIDE         180..189
FT                   /note="Brain peptide NVPIYQEPRF"
FT                   /id="PRO_0000341471"
FT   PEPTIDE         181..189
FT                   /note="Brain peptide VPIYQEPRF"
FT                   /id="PRO_0000341472"
FT   PROPEP          192..319
FT                   /evidence="ECO:0000269|PubMed:17068263"
FT                   /id="PRO_0000341473"
FT   PEPTIDE         322..351
FT                   /note="Brain peptide SQAYDPYSNAAQFQLSSQSRGYPYQHRLVY"
FT                   /id="PRO_0000341474"
FT   PEPTIDE         342..351
FT                   /note="Brain peptide GYPYQHRLVY"
FT                   /id="PRO_0000341475"
FT   REGION          51..71
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          136..176
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        147..176
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   351 AA;  40985 MW;  4FBE69C1306E7A25 CRC64;
     MMCDWVWLLL TLCSLLMIVQ SLPTNLAEDT KKTEQTMRPK SKRAQEMLMF GNQQNHQPEN
     NPSSSYSSTA EKRTLAASGL GGLKAALIEE EKPSRSNTLN NAFYDRKNYD YGAVNELGYE
     IPQVWDNSPY SRYYTNEDRR KRSEKSAVAS GSSTTIKPST TSFQSPTSTQ QSVQTQVKRN
     VPIYQEPRFK RELDIDPEDV LTLLSLWENE RRKRNWHKYM NEEYENVDDE DNLLEEEDSR
     NIIPWMDSSV YPPRHYSLDS LSPSDIGIIR THPSSYYEQY ENQYGQQYDT SQYGSPQYGL
     VYPQQTYYSA PEKRFMISRK RSQAYDPYSN AAQFQLSSQS RGYPYQHRLV Y