PROL4_HUMAN
ID PROL4_HUMAN Reviewed; 134 AA.
AC Q16378; A8KA69; F5H0D7; Q8NFB3;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 3.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Proline-rich protein 4;
DE AltName: Full=Lacrimal proline-rich protein;
DE AltName: Full=Nasopharyngeal carcinoma-associated proline-rich protein 4;
DE Flags: Precursor;
GN Name=PRR4; Synonyms=LPRP, PROL4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND VARIANTS
RP GLN-96 AND ARG-120.
RC TISSUE=Lacrimal gland;
RX PubMed=7544782;
RA Dickinson D.P., Thiesse M.;
RT "A major human lacrimal gland mRNA encodes a new proline-rich protein
RT family member.";
RL Invest. Ophthalmol. Vis. Sci. 36:2020-2031(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Zhang B.-C., Li G.-Y.;
RT "Isolation of differentially expressed genes in nasopharyngeal carcinoma.";
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANTS
RP GLN-96 AND ARG-120.
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS GLN-96 AND
RP ARG-120.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PROTEIN SEQUENCE OF 55-134, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Tear;
RX PubMed=25946035; DOI=10.1021/acs.jproteome.5b00179;
RA Azkargorta M., Soria J., Ojeda C., Guzman F., Acera A., Iloro I.,
RA Suarez T., Elortza F.;
RT "Human basal tear peptidome characterization by CID, HCD, and ETD followed
RT by in silico and in vitro analyses for antimicrobial peptide
RT identification.";
RL J. Proteome Res. 14:2649-2658(2015).
RN [7]
RP PROTEIN SEQUENCE OF 122-134.
RC TISSUE=Platelet;
RX PubMed=12665801; DOI=10.1038/nbt810;
RA Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R.,
RA Vandekerckhove J.;
RT "Exploring proteomes and analyzing protein processing by mass spectrometric
RT identification of sorted N-terminal peptides.";
RL Nat. Biotechnol. 21:566-569(2003).
CC -!- INTERACTION:
CC Q16378; O43681: GET3; NbExp=3; IntAct=EBI-738624, EBI-2515857;
CC Q16378; O43561-2: LAT; NbExp=3; IntAct=EBI-738624, EBI-8070286;
CC Q16378; Q9NQG6: MIEF1; NbExp=3; IntAct=EBI-738624, EBI-740987;
CC Q16378; Q96EQ0: SGTB; NbExp=3; IntAct=EBI-738624, EBI-744081;
CC Q16378; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-738624, EBI-741480;
CC Q16378; Q9UHD9: UBQLN2; NbExp=6; IntAct=EBI-738624, EBI-947187;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q16378-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q16378-2; Sequence=VSP_044635;
CC -!- TISSUE SPECIFICITY: Abundantly expressed in lacrimal gland where it is
CC found in the acinar cells but not in the intralobular ducts. Also found
CC in the submandibular gland, the parotid and sublingual glands.
CC {ECO:0000269|PubMed:7544782}.
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DR EMBL; S79048; AAB35174.1; -; mRNA.
DR EMBL; AF530472; AAM94338.1; -; mRNA.
DR EMBL; AK292934; BAF85623.1; -; mRNA.
DR EMBL; AK292991; BAF85680.1; -; mRNA.
DR EMBL; DB198298; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC006518; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC018630; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC134349; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC217021; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC242308; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC058035; AAH58035.1; -; mRNA.
DR CCDS; CCDS41756.1; -. [Q16378-1]
DR CCDS; CCDS55804.1; -. [Q16378-2]
DR PIR; I54810; I54810.
DR RefSeq; NP_001092008.2; NM_001098538.2. [Q16378-2]
DR RefSeq; NP_009175.2; NM_007244.2. [Q16378-1]
DR AlphaFoldDB; Q16378; -.
DR BioGRID; 116428; 114.
DR IntAct; Q16378; 14.
DR MINT; Q16378; -.
DR STRING; 9606.ENSP00000228811; -.
DR iPTMnet; Q16378; -.
DR PhosphoSitePlus; Q16378; -.
DR BioMuta; PRR4; -.
DR DMDM; 317373525; -.
DR EPD; Q16378; -.
DR jPOST; Q16378; -.
DR MassIVE; Q16378; -.
DR MaxQB; Q16378; -.
DR PaxDb; Q16378; -.
DR PeptideAtlas; Q16378; -.
DR PRIDE; Q16378; -.
DR ProteomicsDB; 60865; -. [Q16378-1]
DR Antibodypedia; 50960; 21 antibodies from 15 providers.
DR DNASU; 11272; -.
DR Ensembl; ENST00000228811.8; ENSP00000228811.4; ENSG00000111215.12. [Q16378-1]
DR Ensembl; ENST00000544994.5; ENSP00000438046.1; ENSG00000111215.12. [Q16378-2]
DR Ensembl; ENST00000574424.5; ENSP00000459468.1; ENSG00000263247.6. [Q16378-1]
DR Ensembl; ENST00000576999.5; ENSP00000461695.1; ENSG00000263247.6. [Q16378-2]
DR Ensembl; ENST00000614435.2; ENSP00000477689.1; ENSG00000282269.1. [Q16378-1]
DR Ensembl; ENST00000632373.1; ENSP00000487830.1; ENSG00000282269.1. [Q16378-2]
DR GeneID; 11272; -.
DR KEGG; hsa:11272; -.
DR MANE-Select; ENST00000228811.8; ENSP00000228811.4; NM_007244.3; NP_009175.2.
DR UCSC; uc001qyz.5; human. [Q16378-1]
DR CTD; 11272; -.
DR DisGeNET; 11272; -.
DR GeneCards; PRR4; -.
DR HGNC; HGNC:18020; PRR4.
DR HPA; ENSG00000111215; Tissue enriched (salivary).
DR MalaCards; PRR4; -.
DR MIM; 605359; gene.
DR neXtProt; NX_Q16378; -.
DR OpenTargets; ENSG00000111215; -.
DR PharmGKB; PA33806; -.
DR VEuPathDB; HostDB:ENSG00000111215; -.
DR eggNOG; ENOG502TED7; Eukaryota.
DR GeneTree; ENSGT00730000112173; -.
DR HOGENOM; CLU_2849034_0_0_1; -.
DR InParanoid; Q16378; -.
DR OMA; HHHYPLP; -.
DR OrthoDB; 1638319at2759; -.
DR PhylomeDB; Q16378; -.
DR PathwayCommons; Q16378; -.
DR SignaLink; Q16378; -.
DR BioGRID-ORCS; 11272; 8 hits in 1072 CRISPR screens.
DR GeneWiki; PRR4; -.
DR GenomeRNAi; 11272; -.
DR Pharos; Q16378; Tbio.
DR PRO; PR:Q16378; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q16378; protein.
DR Bgee; ENSG00000111215; Expressed in olfactory segment of nasal mucosa and 95 other tissues.
DR ExpressionAtlas; Q16378; baseline and differential.
DR Genevisible; Q16378; HS.
DR GO; GO:0005615; C:extracellular space; HDA:UniProtKB.
DR GO; GO:0001895; P:retina homeostasis; HEP:UniProtKB.
DR GO; GO:0007601; P:visual perception; TAS:ProtInc.
DR InterPro; IPR026086; Pro-rich.
DR PANTHER; PTHR23203; PTHR23203; 1.
DR Pfam; PF15240; Pro-rich; 1.
DR SMART; SM01412; Pro-rich; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..134
FT /note="Proline-rich protein 4"
FT /id="PRO_0000022115"
FT REGION 28..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 34..134
FT /note="DVEDSSQRPDQGPQRPPPEGLLPRPPGDSGNQDDGPQQRPPKPGGHHRHPPP
FT PPFQNQQRPPRRGHRQLSLPRFPSVSLQEASSFFQRDRPARHPQEQPLW -> ACRKHH
FT HSSRGTDQQDIPRSNHSGNLEFSGRK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_044635"
FT VARIANT 96
FT /note="R -> Q (in dbSNP:rs1063193)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:7544782"
FT /id="VAR_027926"
FT VARIANT 120
FT /note="Q -> R (in dbSNP:rs1047699)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:7544782"
FT /id="VAR_027927"
FT CONFLICT Q16378-2:43
FT /note="R -> G (in Ref. 3; DB198298)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 134 AA; 15097 MW; 57BB0DCA10938CC8 CRC64;
MLLVLLSVVL LALSSAQSTD NDVNYEDFTF TIPDVEDSSQ RPDQGPQRPP PEGLLPRPPG
DSGNQDDGPQ QRPPKPGGHH RHPPPPPFQN QQRPPRRGHR QLSLPRFPSV SLQEASSFFQ
RDRPARHPQE QPLW
