ID   PRONF_PROFF             Reviewed;         255 AA.
AC   Q6E3K9;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Propionicin-F;
DE   Flags: Precursor;
GN   Name=pcfA {ECO:0000312|EMBL:AAT47515.1};
OS   Propionibacterium freudenreichii subsp. freudenreichii.
OC   Bacteria; Actinobacteria; Propionibacteriales; Propionibacteriaceae;
OC   Propionibacterium.
OX   NCBI_TaxID=66712;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAT47515.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 102-124, FUNCTION,
RP   AND MASS SPECTROMETRY.
RC   STRAIN=LMGT 2946 {ECO:0000312|EMBL:AAT47515.1};
RX   PubMed=15574930; DOI=10.1128/aem.70.12.7303-7310.2004;
RA   Brede D.A., Faye T., Johnsborg O., Oedegaard I., Nes I.F., Holo H.;
RT   "Molecular and genetic characterization of propionicin F, a bacteriocin
RT   from Propionibacterium freudenreichii.";
RL   Appl. Environ. Microbiol. 70:7303-7310(2004).
CC   -!- FUNCTION: Bacteriocin with specific antibacterial activity against
CC       strains of P.freudenreichii. No antibacterial activity was detected
CC       against P.acidipropionici, P.jensenii and P.thoenii.
CC       {ECO:0000269|PubMed:15574930}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: Mass=4397.3; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15574930};
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DR   EMBL; AY587566; AAT47515.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6E3K9; -.
DR   TCDB; 9.B.86.1.1; the propionicin (propionicin) family.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Secreted.
FT   PROPEP          1..101
FT                   /evidence="ECO:0000269|PubMed:15574930"
FT                   /id="PRO_0000229753"
FT   PEPTIDE         102..144
FT                   /note="Propionicin-F"
FT                   /id="PRO_0000229754"
FT   PROPEP          145..255
FT                   /evidence="ECO:0000269|PubMed:15574930"
FT                   /id="PRO_0000229755"
SQ   SEQUENCE   255 AA;  28310 MW;  65482F0161DAD12A CRC64;
     MNTKAVNLKS ENTTKLVSYL TENQLDEFIR RIRIDGALVE EVSQNAKQAL DNTGLNGWIN
     TDCDEGLLSD FISKIASARW IPLAESIRPA VTDRDKYRVS CWFYQGMNIA IYANIGGVAN
     IIGYTEAAVA TLLGAVVAVA PVVPGTPTPP KDKSSQYKEV PLAVRLSETY HEEGVRGLFD
     ELNYSESRMI STLRRASTDG VLINSWNDGQ DTILLKKYNF QDLQLTVRSR IVGNQTIIEE
     CKITDGRKTL SDETV