ID   PROP1_GALME             Reviewed;          37 AA.
AC   P85214;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Proline-rich antimicrobial peptide 1;
OS   Galleria mellonella (Greater wax moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC   Pyralidae; Galleriinae; Galleria.
OX   NCBI_TaxID=7137;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Larval hemolymph {ECO:0000269|PubMed:11995991};
RX   PubMed=11995991;
RA   Mak P., Chmiel D., Gacek G.J.;
RT   "Antibacterial peptides of the moth Galleria mellonella.";
RL   Acta Biochim. Pol. 48:1191-1195(2001).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   INDUCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Larval hemolymph {ECO:0000269|PubMed:17194500};
RX   PubMed=17194500; DOI=10.1016/j.peptides.2006.11.010;
RA   Cytrynska M., Mak P., Zdybicka-Barabas A., Suder P., Jakubowicz T.;
RT   "Purification and characterization of eight peptides from Galleria
RT   mellonella immune hemolymph.";
RL   Peptides 28:533-546(2007).
CC   -!- FUNCTION: Antimicrobial protein. Has antibacterial activity against the
CC       Gram-positive bacterium M.luteus (MIC=55.0 uM). Lacks antibacterial
CC       activity against the Gram-positive bacteria B.circulans, and
CC       L.monocytogenes, and the Gram-negative bacteria E.coli D31 and E.coli
CC       ATCC 25922. Has antifungal activity against P.pastoris (MIC=16.5 uM),
CC       Z.marxianus (MIC=16.5 uM), S.pombe (MIC=11.0 uM), and C.wickerhami
CC       (MIC=16.5 uM), but lacks antifungal activity against A.niger and
CC       C.albidus. {ECO:0000269|PubMed:17194500}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11995991,
CC       ECO:0000269|PubMed:17194500}.
CC   -!- TISSUE SPECIFICITY: Hemolymph. {ECO:0000269|PubMed:11995991,
CC       ECO:0000269|PubMed:17194500}.
CC   -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:11995991,
CC       ECO:0000269|PubMed:17194500}.
CC   -!- MASS SPECTROMETRY: Mass=4322; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:11995991, ECO:0000269|PubMed:17194500};
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DR   AlphaFoldDB; P85214; -.
DR   Proteomes; UP000504614; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide; Immunity;
KW   Innate immunity; Reference proteome; Secreted.
FT   PEPTIDE         1..37
FT                   /note="Proline-rich antimicrobial peptide 1"
FT                   /id="PRO_0000298766"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        20..37
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   37 AA;  4323 MW;  0A2A23BBA316A714 CRC64;
     DIQIPGIKKP THRDIIIPNW NPNVRTQPWQ RFGGNKS