PROP1_MOUSE
ID PROP1_MOUSE Reviewed; 223 AA.
AC P97458; Q5NCG9;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Homeobox protein prophet of Pit-1;
DE Short=PROP-1;
DE AltName: Full=Pituitary-specific homeodomain factor;
GN Name=Prop1; Synonyms=Prop-1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8934515; DOI=10.1038/384327a0;
RA Sornson M.W., Wu W., Dasen J.S., Flynn S.E., Norman D.J., O'Connell S.M.,
RA Gukovsky I., Carriere C., Ryan A.K., Miller A.P., Zuo L., Gleiberman A.S.,
RA Andersen B., Beamer W.G., Rosenfeld M.G.;
RT "Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor
RT defective in Ames dwarfism.";
RL Nature 384:327-333(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP MUTAGENESIS OF SER-83 AND PHE-85.
RX PubMed=10946881; DOI=10.1210/jcem.85.8.6744;
RA Osorio M.G., Kopp P., Marui S., Latronico A.C., Mendonca B.B.,
RA Arnhold I.J.;
RT "Combined pituitary hormone deficiency caused by a novel mutation of a
RT highly conserved residue (F88S) in the homeodomain of PROP-1.";
RL J. Clin. Endocrinol. Metab. 85:2779-2785(2000).
CC -!- FUNCTION: Possibly involved in the ontogenesis of pituitary
CC gonadotropes, as well as somatotropes, lactotropes and caudomedial
CC thyrotropes.
CC -!- INTERACTION:
CC P97458; Q02248: Ctnnb1; NbExp=2; IntAct=EBI-937831, EBI-397872;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; U77946; AAB38884.1; -; mRNA.
DR EMBL; AL627215; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS24643.1; -.
DR RefSeq; NP_032962.1; NM_008936.1.
DR AlphaFoldDB; P97458; -.
DR SMR; P97458; -.
DR BioGRID; 202394; 4.
DR IntAct; P97458; 1.
DR STRING; 10090.ENSMUSP00000057231; -.
DR PhosphoSitePlus; P97458; -.
DR jPOST; P97458; -.
DR PaxDb; P97458; -.
DR PRIDE; P97458; -.
DR Antibodypedia; 29400; 113 antibodies from 24 providers.
DR DNASU; 19127; -.
DR Ensembl; ENSMUST00000051159; ENSMUSP00000057231; ENSMUSG00000044542.
DR GeneID; 19127; -.
DR KEGG; mmu:19127; -.
DR UCSC; uc007itg.1; mouse.
DR CTD; 5626; -.
DR MGI; MGI:109330; Prop1.
DR VEuPathDB; HostDB:ENSMUSG00000044542; -.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000162292; -.
DR HOGENOM; CLU_044912_1_0_1; -.
DR InParanoid; P97458; -.
DR OMA; EPPKSWN; -.
DR OrthoDB; 1351285at2759; -.
DR PhylomeDB; P97458; -.
DR TreeFam; TF315976; -.
DR BioGRID-ORCS; 19127; 2 hits in 72 CRISPR screens.
DR PRO; PR:P97458; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P97458; protein.
DR Bgee; ENSMUSG00000044542; Expressed in adenohypophysis and 10 other tissues.
DR ExpressionAtlas; P97458; baseline and differential.
DR Genevisible; P97458; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0008013; F:beta-catenin binding; IPI:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0008022; F:protein C-terminus binding; IPI:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0021984; P:adenohypophysis development; IMP:MGI.
DR GO; GO:0009887; P:animal organ morphogenesis; IMP:MGI.
DR GO; GO:0006915; P:apoptotic process; IMP:MGI.
DR GO; GO:0001568; P:blood vessel development; IMP:MGI.
DR GO; GO:0016477; P:cell migration; IGI:MGI.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI.
DR GO; GO:0048732; P:gland development; IMP:MGI.
DR GO; GO:0048850; P:hypophysis morphogenesis; IMP:MGI.
DR GO; GO:0021979; P:hypothalamus cell differentiation; IMP:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0021983; P:pituitary gland development; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060126; P:somatotropin secreting cell differentiation; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR042412; PROP1.
DR PANTHER; PTHR47409; PTHR47409; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..223
FT /note="Homeobox protein prophet of Pit-1"
FT /id="PRO_0000049272"
FT DNA_BIND 66..125
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 199..215
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 83
FT /note="S->P: Shows very weak DNA binding to a Prop1
FT response element in gel shift assays; transcriptional
FT activation of a luciferase reporter gene is reduced to 43%
FT compared with that of wild-type Prop1 in transiently
FT transfected human embryonic kidney cells."
FT /evidence="ECO:0000269|PubMed:10946881"
FT MUTAGEN 85
FT /note="F->S: Shows no significant DNA binding to a Prop1
FT response element in gel shift assays; transcriptional
FT activation of a luciferase reporter gene is reduced to
FT approximately 34% compared with that of wild-type Prop1 in
FT transiently transfected human embryonic kidney cells."
FT /evidence="ECO:0000269|PubMed:10946881"
SQ SEQUENCE 223 AA; 25025 MW; CD0AC4DFDC383679 CRC64;
MEAQRRSHQE KQTKGHACGR SLPEPRVASG TLISTVDRSS EAYRRLSGTE LGRPKLCPQR
GRPHSRRRHR TTFNPAQLEQ LESAFGRNQY PDIWAREGLA QDTGLSEARI QVWFQNRRAK
QRKQERSLLQ PIAHLSTATF SGFLPESSAY PYTYGTPPPP APCFPHPYSH SLPSQPSTAA
SLALPPQPED WYPTLHPAPT GHLPCPPPPP MFPLSLETPK SWN
