PROP_ECO57
ID PROP_ECO57 Reviewed; 500 AA.
AC P0C0L8; P30848;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Proline/betaine transporter;
DE AltName: Full=Proline porter II;
DE Short=PPII;
GN Name=proP; OrderedLocusNames=Z5713, ECs5093;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Proton symporter that senses osmotic shifts and responds by
CC importing osmolytes such as proline, glycine betaine, stachydrine,
CC pipecolic acid, ectoine and taurine. It is both an osmosensor and an
CC osmoregulator which is available to participate early in the bacterial
CC osmoregulatory response (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
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DR EMBL; AE005174; AAG59310.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB38516.1; -; Genomic_DNA.
DR PIR; B86106; B86106.
DR PIR; E91265; E91265.
DR RefSeq; NP_313120.1; NC_002695.1.
DR RefSeq; WP_001298520.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0C0L8; -.
DR BMRB; P0C0L8; -.
DR SMR; P0C0L8; -.
DR STRING; 155864.EDL933_5456; -.
DR PRIDE; P0C0L8; -.
DR EnsemblBacteria; AAG59310; AAG59310; Z5713.
DR EnsemblBacteria; BAB38516; BAB38516; ECs_5093.
DR GeneID; 914238; -.
DR KEGG; ece:Z5713; -.
DR KEGG; ecs:ECs_5093; -.
DR PATRIC; fig|386585.9.peg.5323; -.
DR eggNOG; COG0477; Bacteria.
DR HOGENOM; CLU_001265_39_0_6; -.
DR OMA; FLVEMFP; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004736; MHS_symport.
DR InterPro; IPR015041; Osmo_CC.
DR InterPro; IPR036292; ProP_C.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF08946; Osmo_CC; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR SUPFAM; SSF103661; SSF103661; 1.
DR TIGRFAMs; TIGR00883; 2A0106; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Coiled coil; Membrane;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Proline/betaine transporter"
FT /id="PRO_0000050325"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..65
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..97
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..121
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..169
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 191..194
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 282..297
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..325
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..350
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 412..416
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..500
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT COILED 453..498
FT /evidence="ECO:0000255"
SQ SEQUENCE 500 AA; 54846 MW; 60EDDBF61190DDFC CRC64;
MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD
PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD
TIGIWAPILL LICKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA
GVVVLISTIV GEANFLDWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE
GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI
IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV IGLIFAGLLM
LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP
AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE
IADLQAKRTR LVQQHPRIDE
