PROP_SALTY
ID PROP_SALTY Reviewed; 500 AA.
AC P40862;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 13-DEC-2001, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Proline/betaine transporter;
DE AltName: Full=Proline porter II;
DE Short=PPII;
GN Name=proP; OrderedLocusNames=STM4290;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 484-500.
RC STRAIN=LT2;
RX PubMed=8391535; DOI=10.1128/jb.175.13.4154-4164.1993;
RA Roland K.L., Martin L.E., Esther C.R., Spitznagel J.K.;
RT "Spontaneous pmrA mutants of Salmonella typhimurium LT2 define a new two-
RT component regulatory system with a possible role in virulence.";
RL J. Bacteriol. 175:4154-4164(1993).
CC -!- FUNCTION: Proton symporter that senses osmotic shifts and responds by
CC importing osmolytes such as proline, glycine betaine, stachydrine,
CC pipecolic acid, ectoine and taurine. It is both an osmosensor and an
CC osmoregulator which is available to participate early in the bacterial
CC osmoregulatory response (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE006468; AAL23114.1; -; Genomic_DNA.
DR EMBL; L13395; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_463155.1; NC_003197.2.
DR RefSeq; WP_000919710.1; NC_003197.2.
DR AlphaFoldDB; P40862; -.
DR SMR; P40862; -.
DR STRING; 99287.STM4290; -.
DR PaxDb; P40862; -.
DR EnsemblBacteria; AAL23114; AAL23114; STM4290.
DR GeneID; 1255816; -.
DR KEGG; stm:STM4290; -.
DR PATRIC; fig|99287.12.peg.4512; -.
DR HOGENOM; CLU_001265_39_0_6; -.
DR OMA; FLVEMFP; -.
DR PhylomeDB; P40862; -.
DR BioCyc; SENT99287:STM4290-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004736; MHS_symport.
DR InterPro; IPR015041; Osmo_CC.
DR InterPro; IPR036292; ProP_C.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF08946; Osmo_CC; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR SUPFAM; SSF103661; SSF103661; 1.
DR TIGRFAMs; TIGR00883; 2A0106; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Coiled coil; Membrane;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Proline/betaine transporter"
FT /id="PRO_0000050326"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..65
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..97
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..121
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..169
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 191..194
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 282..297
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..325
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..350
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 412..416
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..500
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT COILED 453..498
FT /evidence="ECO:0000255"
SQ SEQUENCE 500 AA; 54787 MW; 84D04CD0DD6461A5 CRC64;
MLKRKKIKPI TLGDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD
PSVQMIAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYA
TIGIWAPILL LLCKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA
GVVVLISTIV GEENFLEWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE
GLQDGPKVSF KEIATKHWRS LLSCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI
IIAIMIGMLF VQPVMGLLSD RFGRRPFVIM GSIALFALAI PAFILINSNV IGLIFAGLLM
LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLIA GLTPTLAAWL VESSQDLMMP
AYYLMVIAVI GLITGISMKE TANRPLKGAT PAASDIQEAK EILGEHYDNI EQKIDDIDQE
IAELQVKRSR LVQQHPRIDE
