ID   PROP_STAA1              Reviewed;         466 AA.
AC   Q9KWK6; A7WZ11;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 3.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=Putative proline/betaine transporter;
GN   Name=proP; OrderedLocusNames=SAHV_0571;
OS   Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=418127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu3 / ATCC 700698;
RX   PubMed=17954695; DOI=10.1128/aac.00534-07;
RA   Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT   "Mutated response regulator graR is responsible for phenotypic conversion
RT   of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT   resistance to vancomycin-intermediate resistance.";
RL   Antimicrob. Agents Chemother. 52:45-53(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 214-253.
RX   PubMed=10708580; DOI=10.1006/bbrc.2000.2277;
RA   Kuroda M., Kuwahara-Arai K., Hiramatsu K.;
RT   "Identification of the up- and down-regulated genes in vancomycin-resistant
RT   Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential
RT   hybridization method.";
RL   Biochem. Biophys. Res. Commun. 269:485-490(2000).
CC   -!- FUNCTION: May be a proton symporter involved in the uptake of osmolytes
CC       such as proline and glycine betaine. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC       Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
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DR   EMBL; AP009324; BAF77454.1; -; Genomic_DNA.
DR   EMBL; AB035449; BAB03326.2; -; Genomic_DNA.
DR   RefSeq; WP_000347061.1; NZ_CTYB01000020.1.
DR   AlphaFoldDB; Q9KWK6; -.
DR   SMR; Q9KWK6; -.
DR   KEGG; saw:SAHV_0571; -.
DR   HOGENOM; CLU_001265_39_5_9; -.
DR   OMA; FLVEMFP; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Symport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..466
FT                   /note="Putative proline/betaine transporter"
FT                   /id="PRO_0000050332"
FT   TRANSMEM        20..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        247..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        313..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        337..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        377..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   466 AA;  51658 MW;  29180E521AC8603D CRC64;
     MDFNKENINM VDAKKAKKTV VATGIGNAME WFDFGVYAYT TAYIGANFFS PVENADIRQM
     LTFAALAIAF LLRPIGGVVF GIIGDKYGRK VVLTSTIILM AFSTLTIGLL PSYDQIGLWA
     PILLLLARVL QGFSTGGEYA GAMTYVAESS PDKRRNSLGS GLEIGTLSGY IAASIMIAVL
     TFFLTDEQMA SFGWRIPFLL GLFLGLFGLY LRRKLEESPV FENDVATQPE RDNINFLQII
     RFYYKDIFVC FVAVVFFNVT NYMVTAYLPT YLEQVIKLDA TTTSVLITCV MAIMIPLALM
     FGKLADKIGE KKVFLIGTGG LTLFSIIAFM LLHSQSFVVI VIGIFILGFF LSTYEATMPG
     SLPTMFYSHI RYRTLSVTFN ISVSIFGGTT PLVATWLVTK TGDPLAPAYY LTAISVIGFL
     VITFLHLSTA GKSLKGSYPN VDNEQDRAYY AEHPKEALWW VKERKN