ID   PROP_STAAR              Reviewed;         466 AA.
AC   Q6GJ96;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Putative proline/betaine transporter;
GN   Name=proP; OrderedLocusNames=SAR0577;
OS   Staphylococcus aureus (strain MRSA252).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282458;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MRSA252;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: May be a proton symporter involved in the uptake of osmolytes
CC       such as proline and glycine betaine. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC       Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
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DR   EMBL; BX571856; CAG39598.1; -; Genomic_DNA.
DR   RefSeq; WP_000347064.1; NC_002952.2.
DR   AlphaFoldDB; Q6GJ96; -.
DR   SMR; Q6GJ96; -.
DR   KEGG; sar:SAR0577; -.
DR   HOGENOM; CLU_001265_39_0_9; -.
DR   OMA; FLVEMFP; -.
DR   OrthoDB; 961781at2; -.
DR   Proteomes; UP000000596; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Symport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..466
FT                   /note="Putative proline/betaine transporter"
FT                   /id="PRO_0000050330"
FT   TRANSMEM        20..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        247..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        313..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        337..356
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        405..425
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   466 AA;  51588 MW;  E13946A235F34C4A CRC64;
     MDFNKENINM VDAKKAKKTV VATGIGNAME WFDFGVYAYT TAYIGANFFS PVESADIRQM
     LTFAALAIAF LLRPIGGVVF GIIGDKYGRK VVLTSTIILM AFSTLTIGLL PSYDQIGLWA
     PILLLLARVL QGFSTGGEYA GAMTYVAESS PDKRRNSLGS GLEIGTLSGY IAASIMIALL
     TFFLTDEQMA SFGWRIPFLL GLFLGLFGLY LRRKLEESPV FENDVATQPE RDNINFLQII
     RFYYKDIFVC FVAVVFFNVT NYMVTAYLPT YLEQVIKLDA TTTSVLITCV MAIMIPLALM
     FGKLADKIGE KKVFLIGTGG LTLFSIIAFM LLHSQSFVVI VIGIFGLGFF LSTYEATMPG
     SLPTMFYSHI RYRTLSVTFN ISVSIFGGTT PLVATWLVAK TGDPLAPAYY LTAISVIGFL
     VITFLHLSTA GKSLKGSYPN VDNEQDRAYY VEHPKEALWW VKERKD