PROT1_ORYSJ
ID PROT1_ORYSJ Reviewed; 473 AA.
AC Q60DN5; A0A0P0W0M9; Q9LUH7;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Proline transporter 1;
DE AltName: Full=OsPROT;
GN Name=PROT1; Synonyms=PROT; OrderedLocusNames=Os03g0644400, LOC_Os03g44230;
GN ORFNames=OSJNBa0038E17.23;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Akibare;
RX PubMed=10945345; DOI=10.1093/pcp/41.6.750;
RA Igarashi Y., Yoshiba Y., Takeshita T., Nomura S., Otomo J.,
RA Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Molecular cloning and characterization of a cDNA encoding proline
RT transporter in rice.";
RL Plant Cell Physiol. 41:750-756(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Proline transporter that mediates proline transport across
CC the plasma membrane when expressed in a heterologous system (Xenopus
CC oocytes). {ECO:0000269|PubMed:10945345}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaf blades and sheaths, stems
CC and young panicle. {ECO:0000269|PubMed:10945345}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.3) subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-43 is the initiator.
CC {ECO:0000305}.
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DR EMBL; AB022783; BAA93437.1; -; mRNA.
DR EMBL; AC145383; AAU90281.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF97856.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12673.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS85459.1; -; Genomic_DNA.
DR EMBL; AK067118; BAG90278.1; -; mRNA.
DR PIR; T50690; T50690.
DR RefSeq; XP_015630391.1; XM_015774905.1.
DR AlphaFoldDB; Q60DN5; -.
DR STRING; 4530.OS03T0644400-01; -.
DR PaxDb; Q60DN5; -.
DR PRIDE; Q60DN5; -.
DR EnsemblPlants; Os03t0644400-01; Os03t0644400-01; Os03g0644400.
DR GeneID; 4333560; -.
DR Gramene; Os03t0644400-01; Os03t0644400-01; Os03g0644400.
DR KEGG; osa:4333560; -.
DR eggNOG; KOG1303; Eukaryota.
DR InParanoid; Q60DN5; -.
DR OMA; HMYIIAM; -.
DR OrthoDB; 570025at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q60DN5; baseline and differential.
DR Genevisible; Q60DN5; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..473
FT /note="Proline transporter 1"
FT /id="PRO_0000418996"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 473 AA; 52054 MW; BB50DA8C106F64FA CRC64;
MDQHQLDEEN QRAALFHSSA PSSSLGADGE EERETVPLLS CKMADDKSDT VQVSEDTAHQ
ISIDPWYQVG FILTTGVNSA YVLGYSASIM VPLGWIGGTC GLILAAAISM YANALLAHLH
EVGGKRHIRY RDLAGHIYGR KMYSLTWALQ YVNLFMINTG LIILAGQALK AIYVLFRDDG
VLKLPYCIAL SGFVCALFAF GIPYLSALRI WLGLSTVFSL IYIMIAFVMS LRDGITTPAK
DYTIPGSHSD RIFTTIGAVA NLVFAYNTGM LPEIQATIRP PVVKNMEKAL WFQFTVGSLP
LYAVTFMGYW AYGSSTSSYL LNSVKGPIWI KTVANLSAFL QTVIALHIFA SPMYEFLDTR
FGSGHGGPFA IHNIMFRVGV RGGYLTVNTL VAAMLPFLGD FMSLTGALST FPLTFVLANH
MYLTVKQNKM SIFRKCWHWL NVVGFSCLSV AAAVAAVRLI TVDYSTYHLF ADM
